Tags

Type your tag names separated by a space and hit enter

Germline Defects Caused by Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms.
PLoS Genet 2016; 12(5):e1006030PG

Abstract

Few animals are known to lay eggs in the absence of ovulation or copulation, as it is presumably energetically wasteful and subjected to negative selection. Characterization of Smed-boule, a member of the DAZ family of germline RNA-binding proteins, revealed that egg capsule (or capsule) production and deposition occurs independently of the presence of gametes in the planarian flatworm Schmidtea mediterranea. Reduction of Smed-boule expression by RNA-interference (RNAi) causes ablation of spermatogonial stem cells and the inability of ovarian germline stem cells to undergo oogenesis. Although animals subjected to Smed-boule RNAi lose their gametes and become sterile, they continue to lay egg capsules. Production of sterile capsules is even observed in virgin Smed-boule(RNAi) and control planarians maintained in complete isolation, demonstrating that egg production in S. mediterranea occurs independently of ovulation, fertilization, or mating. Evidence suggests that this is a conserved feature amongst Platyhelminthes, and therefore relevant to the pathology and dissemination of parasitic flatworms. These findings demonstrate that Smed-boule functions at different stages during male and female germline stem cell development, and also demonstrate that egg capsule production by planarian flatworms occurs independently of signals produced by mating or ova.

Authors+Show Affiliations

Department of Biological Sciences, Wright State University, Dayton, Ohio, United States of America.Department of Biological Sciences, Wright State University, Dayton, Ohio, United States of America.Department of Biological Sciences, Wright State University, Dayton, Ohio, United States of America.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

27149082

Citation

Steiner, Jessica Kathryne, et al. "Germline Defects Caused By Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms." PLoS Genetics, vol. 12, no. 5, 2016, pp. e1006030.
Steiner JK, Tasaki J, Rouhana L. Germline Defects Caused by Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms. PLoS Genet. 2016;12(5):e1006030.
Steiner, J. K., Tasaki, J., & Rouhana, L. (2016). Germline Defects Caused by Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms. PLoS Genetics, 12(5), pp. e1006030. doi:10.1371/journal.pgen.1006030.
Steiner JK, Tasaki J, Rouhana L. Germline Defects Caused By Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms. PLoS Genet. 2016;12(5):e1006030. PubMed PMID: 27149082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Germline Defects Caused by Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms. AU - Steiner,Jessica Kathryne, AU - Tasaki,Junichi, AU - Rouhana,Labib, Y1 - 2016/05/05/ PY - 2015/12/15/received PY - 2016/04/15/accepted PY - 2016/5/6/entrez PY - 2016/5/7/pubmed PY - 2017/5/13/medline SP - e1006030 EP - e1006030 JF - PLoS genetics JO - PLoS Genet. VL - 12 IS - 5 N2 - Few animals are known to lay eggs in the absence of ovulation or copulation, as it is presumably energetically wasteful and subjected to negative selection. Characterization of Smed-boule, a member of the DAZ family of germline RNA-binding proteins, revealed that egg capsule (or capsule) production and deposition occurs independently of the presence of gametes in the planarian flatworm Schmidtea mediterranea. Reduction of Smed-boule expression by RNA-interference (RNAi) causes ablation of spermatogonial stem cells and the inability of ovarian germline stem cells to undergo oogenesis. Although animals subjected to Smed-boule RNAi lose their gametes and become sterile, they continue to lay egg capsules. Production of sterile capsules is even observed in virgin Smed-boule(RNAi) and control planarians maintained in complete isolation, demonstrating that egg production in S. mediterranea occurs independently of ovulation, fertilization, or mating. Evidence suggests that this is a conserved feature amongst Platyhelminthes, and therefore relevant to the pathology and dissemination of parasitic flatworms. These findings demonstrate that Smed-boule functions at different stages during male and female germline stem cell development, and also demonstrate that egg capsule production by planarian flatworms occurs independently of signals produced by mating or ova. SN - 1553-7404 UR - https://www.unboundmedicine.com/medline/citation/27149082/Germline_Defects_Caused_by_Smed_boule_RNA_Interference_Reveal_That_Egg_Capsule_Deposition_Occurs_Independently_of_Fertilization_Ovulation_Mating_or_the_Presence_of_Gametes_in_Planarian_Flatworms_ L2 - http://dx.plos.org/10.1371/journal.pgen.1006030 DB - PRIME DP - Unbound Medicine ER -