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Magnetic immunoassay for cancer biomarker detection based on surface-enhanced resonance Raman scattering from coupled plasmonic nanostructures.
Biosens Bioelectron. 2016 Oct 15; 84:15-21.BB

Abstract

A surface-enhanced resonance Raman scattering (SERRS) sensor was developed for the ultrasensitive detection of cancer biomarkers. Capture antibody-coated silver shell magnetic nanoparticles (Fe3O4@Ag MNPs) were utilized as the CEA enrichment platform and the SERRS signal amplification substrate. Gold nanorods (AuNRs) were coated with a thin silver shell to be in resonance with the resonant Raman dye diethylthiatricarbocyanine iodide (DTTC) and the excitation wavelength at 785nm. The silver-coated AuNRs (Au@Ag NRs) were then modified with detection antibody as the SERRS tags. Sandwich immune complexes formed in the presence of the target biomarker carcinoembryonic antigen (CEA), and this formation induced the plasmonic coupling between the Au@Ag NRs and Fe3O4@Ag MNPs. The SERRS signal of DTTC molecules located in the coupled plasmonic nanostructures was significantly enhanced. As a result, the proposed SERRS sensor was able to detect CEA with a low limit of detection of 4.75fg/mL and a wide dynamic linear range from 10fg/mL to 100ng/mL. The sensor provides a novel SERRS strategy for trace analyte detection and has a potential for clinical applications.

Authors+Show Affiliations

Beijing Institute of Radiation Medicine, Beijing 100850, PR China.Beijing Institute of Radiation Medicine, Beijing 100850, PR China; College of Life Sciences & Bio-Engineering, Beijing University of Technology, Beijing 100124, PR China.College of Mechatronics and Automation, National University of Defense Technology, Changsha 410073, PR China.Institute of Transfusion Medicine, Beijing 100850, PR China. Electronic address: David_king@139.com.Beijing Institute of Radiation Medicine, Beijing 100850, PR China. Electronic address: ruixiao203@sina.com.Beijing Institute of Radiation Medicine, Beijing 100850, PR China. Electronic address: sqwang@bmi.ac.cn.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

27149164

Citation

Rong, Zhen, et al. "Magnetic Immunoassay for Cancer Biomarker Detection Based On Surface-enhanced Resonance Raman Scattering From Coupled Plasmonic Nanostructures." Biosensors & Bioelectronics, vol. 84, 2016, pp. 15-21.
Rong Z, Wang C, Wang J, et al. Magnetic immunoassay for cancer biomarker detection based on surface-enhanced resonance Raman scattering from coupled plasmonic nanostructures. Biosens Bioelectron. 2016;84:15-21.
Rong, Z., Wang, C., Wang, J., Wang, D., Xiao, R., & Wang, S. (2016). Magnetic immunoassay for cancer biomarker detection based on surface-enhanced resonance Raman scattering from coupled plasmonic nanostructures. Biosensors & Bioelectronics, 84, 15-21. https://doi.org/10.1016/j.bios.2016.04.006
Rong Z, et al. Magnetic Immunoassay for Cancer Biomarker Detection Based On Surface-enhanced Resonance Raman Scattering From Coupled Plasmonic Nanostructures. Biosens Bioelectron. 2016 Oct 15;84:15-21. PubMed PMID: 27149164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Magnetic immunoassay for cancer biomarker detection based on surface-enhanced resonance Raman scattering from coupled plasmonic nanostructures. AU - Rong,Zhen, AU - Wang,Chongwen, AU - Wang,Junfeng, AU - Wang,Donggen, AU - Xiao,Rui, AU - Wang,Shengqi, Y1 - 2016/04/05/ PY - 2016/02/04/received PY - 2016/03/23/revised PY - 2016/04/04/accepted PY - 2016/5/6/entrez PY - 2016/5/6/pubmed PY - 2017/2/1/medline KW - Au@Ag nanorod KW - Carcinoembryonic antigen KW - Coupled plasmonic nanostructure KW - Fe(3)O(4)@Ag magnetic nanoparticle KW - Surface-enhanced resonance Raman scattering SP - 15 EP - 21 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 84 N2 - A surface-enhanced resonance Raman scattering (SERRS) sensor was developed for the ultrasensitive detection of cancer biomarkers. Capture antibody-coated silver shell magnetic nanoparticles (Fe3O4@Ag MNPs) were utilized as the CEA enrichment platform and the SERRS signal amplification substrate. Gold nanorods (AuNRs) were coated with a thin silver shell to be in resonance with the resonant Raman dye diethylthiatricarbocyanine iodide (DTTC) and the excitation wavelength at 785nm. The silver-coated AuNRs (Au@Ag NRs) were then modified with detection antibody as the SERRS tags. Sandwich immune complexes formed in the presence of the target biomarker carcinoembryonic antigen (CEA), and this formation induced the plasmonic coupling between the Au@Ag NRs and Fe3O4@Ag MNPs. The SERRS signal of DTTC molecules located in the coupled plasmonic nanostructures was significantly enhanced. As a result, the proposed SERRS sensor was able to detect CEA with a low limit of detection of 4.75fg/mL and a wide dynamic linear range from 10fg/mL to 100ng/mL. The sensor provides a novel SERRS strategy for trace analyte detection and has a potential for clinical applications. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/27149164/Magnetic_immunoassay_for_cancer_biomarker_detection_based_on_surface_enhanced_resonance_Raman_scattering_from_coupled_plasmonic_nanostructures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(16)30282-2 DB - PRIME DP - Unbound Medicine ER -