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Chikusetsusaponin IVa Methyl Ester Isolated from the Roots of Achyranthes japonica Suppresses LPS-Induced iNOS, TNF-α, IL-6, and IL-1β Expression by NF-κB and AP-1 Inactivation.
Biol Pharm Bull. 2016; 39(5):657-64.BP

Abstract

We investigated the effect of chikusetsusaponin IVa (CS) and chikusetsusaponin IVa methyl ester (CS-ME) from the roots of Achyranthes japonica NAKAI on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW264.7 macrophages. CS-ME more potently inhibited LPS-induced NO and PGE2 production than CS. CS-ME concentration-dependently inhibited LPS-induced tumor necrosis factor (TNF)-α and interleukin (IL)-6 and IL-1β production in RAW264.7 macrophages and mouse peritoneal macrophages. Consistent with these findings, CS-ME suppressed LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 at protein level as well as iNOS, COX-2, TNF-α, IL-6, and IL-1β at mRNA level. In addition, CS-ME suppressed LPS-induced transcriptional activity of nuclear factor (NF)-κB and activator protein (AP)-1. The anti-inflammatory properties of CS-ME might result from suppression of iNOS, COX-2, TNF-α, IL-6, and IL-1β expression through downregulation of NF-κB and AP-1 in macrophages.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27150139

Citation

Lee, Hae-Jun, et al. "Chikusetsusaponin IVa Methyl Ester Isolated From the Roots of Achyranthes Japonica Suppresses LPS-Induced iNOS, TNF-α, IL-6, and IL-1β Expression By NF-κB and AP-1 Inactivation." Biological & Pharmaceutical Bulletin, vol. 39, no. 5, 2016, pp. 657-64.
Lee HJ, Shin JS, Lee WS, et al. Chikusetsusaponin IVa Methyl Ester Isolated from the Roots of Achyranthes japonica Suppresses LPS-Induced iNOS, TNF-α, IL-6, and IL-1β Expression by NF-κB and AP-1 Inactivation. Biol Pharm Bull. 2016;39(5):657-64.
Lee, H. J., Shin, J. S., Lee, W. S., Shim, H. Y., Park, J. M., Jang, D. S., & Lee, K. T. (2016). Chikusetsusaponin IVa Methyl Ester Isolated from the Roots of Achyranthes japonica Suppresses LPS-Induced iNOS, TNF-α, IL-6, and IL-1β Expression by NF-κB and AP-1 Inactivation. Biological & Pharmaceutical Bulletin, 39(5), 657-64. https://doi.org/10.1248/bpb.b15-00572
Lee HJ, et al. Chikusetsusaponin IVa Methyl Ester Isolated From the Roots of Achyranthes Japonica Suppresses LPS-Induced iNOS, TNF-α, IL-6, and IL-1β Expression By NF-κB and AP-1 Inactivation. Biol Pharm Bull. 2016;39(5):657-64. PubMed PMID: 27150139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chikusetsusaponin IVa Methyl Ester Isolated from the Roots of Achyranthes japonica Suppresses LPS-Induced iNOS, TNF-α, IL-6, and IL-1β Expression by NF-κB and AP-1 Inactivation. AU - Lee,Hae-Jun, AU - Shin,Ji-Sun, AU - Lee,Woo-Seok, AU - Shim,Heon-Yong, AU - Park,Ji-Min, AU - Jang,Dae-Sik, AU - Lee,Kyung-Tae, PY - 2016/5/7/entrez PY - 2016/5/7/pubmed PY - 2017/1/28/medline SP - 657 EP - 64 JF - Biological & pharmaceutical bulletin JO - Biol Pharm Bull VL - 39 IS - 5 N2 - We investigated the effect of chikusetsusaponin IVa (CS) and chikusetsusaponin IVa methyl ester (CS-ME) from the roots of Achyranthes japonica NAKAI on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW264.7 macrophages. CS-ME more potently inhibited LPS-induced NO and PGE2 production than CS. CS-ME concentration-dependently inhibited LPS-induced tumor necrosis factor (TNF)-α and interleukin (IL)-6 and IL-1β production in RAW264.7 macrophages and mouse peritoneal macrophages. Consistent with these findings, CS-ME suppressed LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 at protein level as well as iNOS, COX-2, TNF-α, IL-6, and IL-1β at mRNA level. In addition, CS-ME suppressed LPS-induced transcriptional activity of nuclear factor (NF)-κB and activator protein (AP)-1. The anti-inflammatory properties of CS-ME might result from suppression of iNOS, COX-2, TNF-α, IL-6, and IL-1β expression through downregulation of NF-κB and AP-1 in macrophages. SN - 1347-5215 UR - https://www.unboundmedicine.com/medline/citation/27150139/Chikusetsusaponin_IVa_Methyl_Ester_Isolated_from_the_Roots_of_Achyranthes_japonica_Suppresses_LPS_Induced_iNOS_TNF_α_IL_6_and_IL_1β_Expression_by_NF_κB_and_AP_1_Inactivation_ L2 - https://dx.doi.org/10.1248/bpb.b15-00572 DB - PRIME DP - Unbound Medicine ER -