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Synthesis of silver nanoparticles from two acidophilic strains of Pilimelia columellifera subsp. pallida and their antibacterial activities.
J Basic Microbiol. 2016 May; 56(5):541-56.JB

Abstract

Biosynthesis of silver nanoparticles (AgNPs) is an eco-friendly approach by using different biological sources; for example, plants and microorganisms such as bacteria, fungi, and actinobacteria. In this report, we present the biological synthesis of silver nanoparticles (AgNPs) by acidophilic actinomycetes SL19 and SL24 strains isolated from pine forest soil (pH < 4.0). The isolates based on 16S rRNA gene sequence were identified as Pilimelia columellifera subsp. pallida. The synthesized AgNPs were characterized by visual observations of colour change from light-yellow to dark-brown. The UV-vis spectra of AgNPs were recorded at 425 and 430 nm. The AgNPs were further characterized by Nanoparticle tracking analysis (NTA), Zeta potential, Fourier transform infrared spectroscopy (FTIR) and Transmission electron microscopy (TEM). FTIR analysis revealed the presence of proteins as a capping agent. TEM analysis confirmed the formation of spherical and polydispersed NPs of 12.7 and 15.9 nm sizes. The in vitro antibacterial activity of AgNPs alone and in combination with antibiotics was evaluated against clinical bacteria viz., Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and uropathogens such as Enterobacter, S. aureus, P. aeruginosa, K. pneumoniae, and E. coli. The lowest MIC (40 μg ml(-1)) was demonstrated by AgNPs synthesized from SL24 against E. coli. However, the AgNPs of SL19 showed lowest MIC (70 μg ml(-1)) against S. aureus. The activity of antibiotic was enhanced, when tested in combination with silver nanoparticles synthesized from both actinobacterial strains.

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Authors+Show Affiliations

Golińska P
Department of Microbiology, Nicolaus Copernicus University, Torun, Poland.
Wypij M
Department of Microbiology, Nicolaus Copernicus University, Torun, Poland.
Rathod D
Department of Microbiology, Nicolaus Copernicus University, Torun, Poland. Department of Biotechnology, Nanobiotechnology Laboratory, SGB Amravati University, Amravati, Maharashtra, India.
Tikar S
Department of Biotechnology, Nanobiotechnology Laboratory, SGB Amravati University, Amravati, Maharashtra, India.
Dahm H
Department of Microbiology, Nicolaus Copernicus University, Torun, Poland.
Rai M
Department of Biotechnology, Nanobiotechnology Laboratory, SGB Amravati University, Amravati, Maharashtra, India.

MeSH

Anti-Bacterial AgentsBacillus subtilisBacteriaEscherichia coliKlebsiella pneumoniaeMetal NanoparticlesMicrobial Sensitivity TestsMicromonosporaceaeMicroscopy, Electron, TransmissionPseudomonas aeruginosaRNA, Ribosomal, 16SSilverSoil MicrobiologyStaphylococcus aureus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27151174

Citation

Golińska, Patrycja, et al. "Synthesis of Silver Nanoparticles From Two Acidophilic Strains of Pilimelia Columellifera Subsp. Pallida and Their Antibacterial Activities." Journal of Basic Microbiology, vol. 56, no. 5, 2016, pp. 541-56.
Golińska P, Wypij M, Rathod D, et al. Synthesis of silver nanoparticles from two acidophilic strains of Pilimelia columellifera subsp. pallida and their antibacterial activities. J Basic Microbiol. 2016;56(5):541-56.
Golińska, P., Wypij, M., Rathod, D., Tikar, S., Dahm, H., & Rai, M. (2016). Synthesis of silver nanoparticles from two acidophilic strains of Pilimelia columellifera subsp. pallida and their antibacterial activities. Journal of Basic Microbiology, 56(5), 541-56. https://doi.org/10.1002/jobm.201500516
Golińska P, et al. Synthesis of Silver Nanoparticles From Two Acidophilic Strains of Pilimelia Columellifera Subsp. Pallida and Their Antibacterial Activities. J Basic Microbiol. 2016;56(5):541-56. PubMed PMID: 27151174.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis of silver nanoparticles from two acidophilic strains of Pilimelia columellifera subsp. pallida and their antibacterial activities. AU - Golińska,Patrycja, AU - Wypij,Magdalena, AU - Rathod,Dnyaneshwar, AU - Tikar,Sagar, AU - Dahm,Hanna, AU - Rai,Mahendra, Y1 - 2015/12/21/ PY - 2015/08/17/received PY - 2015/12/02/accepted PY - 2016/5/7/entrez PY - 2016/5/7/pubmed PY - 2017/2/18/medline KW - Acidophilic actinobacteria KW - Biosynthesis KW - Clinical pathogens KW - Silver nanoparticles KW - Uropathogens SP - 541 EP - 56 JF - Journal of basic microbiology JO - J Basic Microbiol VL - 56 IS - 5 N2 - Biosynthesis of silver nanoparticles (AgNPs) is an eco-friendly approach by using different biological sources; for example, plants and microorganisms such as bacteria, fungi, and actinobacteria. In this report, we present the biological synthesis of silver nanoparticles (AgNPs) by acidophilic actinomycetes SL19 and SL24 strains isolated from pine forest soil (pH < 4.0). The isolates based on 16S rRNA gene sequence were identified as Pilimelia columellifera subsp. pallida. The synthesized AgNPs were characterized by visual observations of colour change from light-yellow to dark-brown. The UV-vis spectra of AgNPs were recorded at 425 and 430 nm. The AgNPs were further characterized by Nanoparticle tracking analysis (NTA), Zeta potential, Fourier transform infrared spectroscopy (FTIR) and Transmission electron microscopy (TEM). FTIR analysis revealed the presence of proteins as a capping agent. TEM analysis confirmed the formation of spherical and polydispersed NPs of 12.7 and 15.9 nm sizes. The in vitro antibacterial activity of AgNPs alone and in combination with antibiotics was evaluated against clinical bacteria viz., Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and uropathogens such as Enterobacter, S. aureus, P. aeruginosa, K. pneumoniae, and E. coli. The lowest MIC (40 μg ml(-1)) was demonstrated by AgNPs synthesized from SL24 against E. coli. However, the AgNPs of SL19 showed lowest MIC (70 μg ml(-1)) against S. aureus. The activity of antibiotic was enhanced, when tested in combination with silver nanoparticles synthesized from both actinobacterial strains. SN - 1521-4028 UR - https://www.unboundmedicine.com/medline/citation/27151174/Synthesis_of_silver_nanoparticles_from_two_acidophilic_strains_of_Pilimelia_columellifera_subsp__pallida_and_their_antibacterial_activities_ DB - PRIME DP - Unbound Medicine ER -
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