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The Wound Healing Effects of Adiponectin Eye Drops after Corneal Alkali Burn.
Curr Eye Res. 2016 11; 41(11):1424-1432.CE

Abstract

PURPOSE

To investigate the wound healing effect of adiponectin eye drops following corneal alkali burn.

MATERIALS AND METHODS

A chemical burn was induced using 0.1 M NaOH in both adenovirus 12-SV40 hybrid-transformed human corneal epithelial (HCE-2) cells and C57BL/6 mice. The injured HCE-2 and mice were then treated using either 0.1% hyaluronic acid (HA) or adiponectin at 0.0001%, 0.001%, or 0.01% concentration. The viability of the HCE-2 cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The wound healing rate of the HCE-2 cells was evaluated using a migration assay 4, 8, 12, 24, and 48 h after chemical injury. In mice, corneal epithelial defects and degree of haze were analyzed 6 h and 1, 2, 3, 6, and 7 days after chemical injury. Seven days after injury, the concentrations of IL-1β and transforming growth factor-β (TGF-β) in the cornea were measured using enzyme-linked immunosorbent assay, and histological analysis was also performed.

RESULTS

The viability of HCE-2 cells was not affected by adiponectin at any of the concentrations used. In HCE-2 cells treated using either 0.001% or 0.01% adiponectin, the wound healing rate after 4 h was significantly faster than in the control and HA-treated groups. With regard to mice, the 0.001% and 0.01% adiponectin-treated groups showed a significant improvement in epithelial defect parameters and haze scores at 3, 5, and 7 days after chemical injury. A significant decrease in IL-1β and TGF-β levels was observed in the 0.001% and 0.01% adiponectin-treated groups compared to the other groups. Histologically, corneal thickness and the inflammatory cells were also decreased in the adiponectin-treated groups.

CONCLUSIONS

Topical adiponectin (both 0.001% and 0.01%) increased epithelial migration and improved clinical signs and inflammation on the ocular surface after alkali burn, suggesting that adiponectin can promote wound healing in the cornea.

Authors+Show Affiliations

a Xiamen Eye Center of Xiamen University , Xiamen , Fujian , China. b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.c Department of Ophthalmology , Ulsan University Hospital, University of Ulsan College of Medicine , Ulsan , South Korea.d Microbiology and Functionality Research Group, R & D Division , World Institute of Kimchi , Gwangju , South Korea.b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27159062

Citation

Li, Zhengri, et al. "The Wound Healing Effects of Adiponectin Eye Drops After Corneal Alkali Burn." Current Eye Research, vol. 41, no. 11, 2016, pp. 1424-1432.
Li Z, Cui L, Yang JM, et al. The Wound Healing Effects of Adiponectin Eye Drops after Corneal Alkali Burn. Curr Eye Res. 2016;41(11):1424-1432.
Li, Z., Cui, L., Yang, J. M., Lee, H. S., Choi, J. S., Woo, J. M., Lim, S. K., & Yoon, K. C. (2016). The Wound Healing Effects of Adiponectin Eye Drops after Corneal Alkali Burn. Current Eye Research, 41(11), 1424-1432.
Li Z, et al. The Wound Healing Effects of Adiponectin Eye Drops After Corneal Alkali Burn. Curr Eye Res. 2016;41(11):1424-1432. PubMed PMID: 27159062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Wound Healing Effects of Adiponectin Eye Drops after Corneal Alkali Burn. AU - Li,Zhengri, AU - Cui,Lian, AU - Yang,Jee Myung, AU - Lee,Hyo Seok, AU - Choi,Ji Suk, AU - Woo,Je Moon, AU - Lim,Seul Ki, AU - Yoon,Kyung Chul, Y1 - 2016/05/09/ PY - 2016/5/10/pubmed PY - 2017/10/19/medline PY - 2016/5/10/entrez KW - Adiponectin KW - corneal alkali burn KW - corneal epithelial cells KW - inflammation KW - wound healing SP - 1424 EP - 1432 JF - Current eye research JO - Curr Eye Res VL - 41 IS - 11 N2 - PURPOSE: To investigate the wound healing effect of adiponectin eye drops following corneal alkali burn. MATERIALS AND METHODS: A chemical burn was induced using 0.1 M NaOH in both adenovirus 12-SV40 hybrid-transformed human corneal epithelial (HCE-2) cells and C57BL/6 mice. The injured HCE-2 and mice were then treated using either 0.1% hyaluronic acid (HA) or adiponectin at 0.0001%, 0.001%, or 0.01% concentration. The viability of the HCE-2 cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The wound healing rate of the HCE-2 cells was evaluated using a migration assay 4, 8, 12, 24, and 48 h after chemical injury. In mice, corneal epithelial defects and degree of haze were analyzed 6 h and 1, 2, 3, 6, and 7 days after chemical injury. Seven days after injury, the concentrations of IL-1β and transforming growth factor-β (TGF-β) in the cornea were measured using enzyme-linked immunosorbent assay, and histological analysis was also performed. RESULTS: The viability of HCE-2 cells was not affected by adiponectin at any of the concentrations used. In HCE-2 cells treated using either 0.001% or 0.01% adiponectin, the wound healing rate after 4 h was significantly faster than in the control and HA-treated groups. With regard to mice, the 0.001% and 0.01% adiponectin-treated groups showed a significant improvement in epithelial defect parameters and haze scores at 3, 5, and 7 days after chemical injury. A significant decrease in IL-1β and TGF-β levels was observed in the 0.001% and 0.01% adiponectin-treated groups compared to the other groups. Histologically, corneal thickness and the inflammatory cells were also decreased in the adiponectin-treated groups. CONCLUSIONS: Topical adiponectin (both 0.001% and 0.01%) increased epithelial migration and improved clinical signs and inflammation on the ocular surface after alkali burn, suggesting that adiponectin can promote wound healing in the cornea. SN - 1460-2202 UR - https://www.unboundmedicine.com/medline/citation/27159062/The_Wound_Healing_Effects_of_Adiponectin_Eye_Drops_after_Corneal_Alkali_Burn_ DB - PRIME DP - Unbound Medicine ER -