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Dissecting stylar responses to self-pollination in wild tomato self-compatible and self-incompatible species using comparative proteomics.
Plant Physiol Biochem 2016; 106:177-86PP

Abstract

Self-incompatibility (SI), a phenomenon that is widespread among flowering plants (angiosperms), promotes outbreeding, resulting in increased genetic diversity and species survival. SI is also important in establishing intra- or interspecies reproductive barriers, such as those that are evident in the tomato clade, Solanum section Lycopersicon, where they limit the use of wild species inbreeding programs to improve cultivated tomato. However, the molecular mechanisms underlying SI are poorly understood in the tomato clade. In this study, an SI (Solanum chilense, LA0130) and a self-compatible (SC, Solanum pimpinellifolium, LA1585) tomato species were chosen to dissect the mechanism of SI formation using a comparative proteomics approach. A total of 635 and 627 protein spots were detected in two-dimensional electrophoresis (2-DE) maps of proteins from the SI and SC species, respectively. In the SC species, 22 differently expressed proteins (DEPs) were detected in SCP versus SCUP (self-pollination versus non-pollination in SC species). Of these, 3 and 18 showed an up-or down-regulated expression in the SCP protein sample, respectively, while only one DEP (MSRA, Solyc03g111720) was exclusively expressed in the SCP sample. In the SI species, 14 DEPs were found between SIP/SIUP, and 5 of these showed higher expression in SIP, whereas two DEPs (MLP-like protein 423-like, gene ID, 460386008 and (ATP synthase subunit alpha, gene ID, Solyc00g042130) were exclusively expressed in SIP or SIUP, respectively. Finally, two S-RNases (gene IDs, 313247946 and 157377662) were exclusively expressed in the SI species. Sequence homology analysis and a gene ontology tool were used to assign the DEPs to the 'metabolism', 'energy', 'cytoskeleton dynamics', 'protein degradation', 'signal transduction', 'defence/stress responses', 'self-incompatibility' and 'unknown' protein categories. We discuss the putative functions of the DEPs in different biological processes and how these might be associated with the regulation of SI formation in the tomato clade.

Authors+Show Affiliations

Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.Department of Environment Resource, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: lxzhao@sjtu.edu.cn.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27163628

Citation

Zhao, Panfeng, et al. "Dissecting Stylar Responses to Self-pollination in Wild Tomato Self-compatible and Self-incompatible Species Using Comparative Proteomics." Plant Physiology and Biochemistry : PPB, vol. 106, 2016, pp. 177-86.
Zhao P, Wang M, Zhao L. Dissecting stylar responses to self-pollination in wild tomato self-compatible and self-incompatible species using comparative proteomics. Plant Physiol Biochem. 2016;106:177-86.
Zhao, P., Wang, M., & Zhao, L. (2016). Dissecting stylar responses to self-pollination in wild tomato self-compatible and self-incompatible species using comparative proteomics. Plant Physiology and Biochemistry : PPB, 106, pp. 177-86. doi:10.1016/j.plaphy.2016.05.001.
Zhao P, Wang M, Zhao L. Dissecting Stylar Responses to Self-pollination in Wild Tomato Self-compatible and Self-incompatible Species Using Comparative Proteomics. Plant Physiol Biochem. 2016;106:177-86. PubMed PMID: 27163628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissecting stylar responses to self-pollination in wild tomato self-compatible and self-incompatible species using comparative proteomics. AU - Zhao,Panfeng, AU - Wang,Meng, AU - Zhao,Lingxia, Y1 - 2016/05/02/ PY - 2015/10/14/received PY - 2016/04/30/revised PY - 2016/05/01/accepted PY - 2016/5/11/entrez PY - 2016/5/11/pubmed PY - 2017/3/28/medline KW - Proteomics KW - Self-compatibility KW - Self-incompatibility KW - Style KW - Tomato SP - 177 EP - 86 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol. Biochem. VL - 106 N2 - Self-incompatibility (SI), a phenomenon that is widespread among flowering plants (angiosperms), promotes outbreeding, resulting in increased genetic diversity and species survival. SI is also important in establishing intra- or interspecies reproductive barriers, such as those that are evident in the tomato clade, Solanum section Lycopersicon, where they limit the use of wild species inbreeding programs to improve cultivated tomato. However, the molecular mechanisms underlying SI are poorly understood in the tomato clade. In this study, an SI (Solanum chilense, LA0130) and a self-compatible (SC, Solanum pimpinellifolium, LA1585) tomato species were chosen to dissect the mechanism of SI formation using a comparative proteomics approach. A total of 635 and 627 protein spots were detected in two-dimensional electrophoresis (2-DE) maps of proteins from the SI and SC species, respectively. In the SC species, 22 differently expressed proteins (DEPs) were detected in SCP versus SCUP (self-pollination versus non-pollination in SC species). Of these, 3 and 18 showed an up-or down-regulated expression in the SCP protein sample, respectively, while only one DEP (MSRA, Solyc03g111720) was exclusively expressed in the SCP sample. In the SI species, 14 DEPs were found between SIP/SIUP, and 5 of these showed higher expression in SIP, whereas two DEPs (MLP-like protein 423-like, gene ID, 460386008 and (ATP synthase subunit alpha, gene ID, Solyc00g042130) were exclusively expressed in SIP or SIUP, respectively. Finally, two S-RNases (gene IDs, 313247946 and 157377662) were exclusively expressed in the SI species. Sequence homology analysis and a gene ontology tool were used to assign the DEPs to the 'metabolism', 'energy', 'cytoskeleton dynamics', 'protein degradation', 'signal transduction', 'defence/stress responses', 'self-incompatibility' and 'unknown' protein categories. We discuss the putative functions of the DEPs in different biological processes and how these might be associated with the regulation of SI formation in the tomato clade. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/27163628/Dissecting_stylar_responses_to_self_pollination_in_wild_tomato_self_compatible_and_self_incompatible_species_using_comparative_proteomics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30171-1 DB - PRIME DP - Unbound Medicine ER -