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Application of a quantitative (1)H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan.
J Nat Med. 2016 Oct; 70(4):797-802.JN

Abstract

Quantitative (1)H-NMR ((1)H-qNMR) was applied to the determination of paeonol concentration in Moutan cortex, Hachimijiogan, and Keishibukuryogan. Paeonol is a major component of Moutan cortex, and its purity was calculated from the ratio of the intensity of the paeonol H-3' signal at δ 6.41 ppm in methanol-d 4 or 6.40 ppm in methanol-d 4 + TFA-d to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material grade. As a result, the paeonol content in two lots of Moutan cortex as determined by (1)H-qNMR was found to be 1.59 % and 1.62 %, respectively, while the paeonol content in Hachimijiogan and Keishibukuryogan was 0.15 % and 0.22 %, respectively. The present study demonstrated that the (1)H-NMR method is useful for the quantitative analysis of crude drugs and Kampo formulas.

Authors+Show Affiliations

Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan. National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan. anagatsu@kinjo-u.ac.jp.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

27164909

Citation

Tanaka, Rie, et al. "Application of a Quantitative (1)H-NMR Method for the Determination of Paeonol in Moutan Cortex, Hachimijiogan and Keishibukuryogan." Journal of Natural Medicines, vol. 70, no. 4, 2016, pp. 797-802.
Tanaka R, Shibata H, Sugimoto N, et al. Application of a quantitative (1)H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan. J Nat Med. 2016;70(4):797-802.
Tanaka, R., Shibata, H., Sugimoto, N., Akiyama, H., & Nagatsu, A. (2016). Application of a quantitative (1)H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan. Journal of Natural Medicines, 70(4), 797-802. https://doi.org/10.1007/s11418-016-1003-3
Tanaka R, et al. Application of a Quantitative (1)H-NMR Method for the Determination of Paeonol in Moutan Cortex, Hachimijiogan and Keishibukuryogan. J Nat Med. 2016;70(4):797-802. PubMed PMID: 27164909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of a quantitative (1)H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan. AU - Tanaka,Rie, AU - Shibata,Hikari, AU - Sugimoto,Naoki, AU - Akiyama,Hiroshi, AU - Nagatsu,Akito, Y1 - 2016/05/10/ PY - 2016/02/24/received PY - 2016/04/19/accepted PY - 2016/5/12/entrez PY - 2016/5/12/pubmed PY - 2017/3/23/medline KW - Hachimijiogan KW - Keishibukuryogan KW - Moutan cortex KW - Paeonol KW - Quantitative 1H-NMR KW - Reagent purity SP - 797 EP - 802 JF - Journal of natural medicines JO - J Nat Med VL - 70 IS - 4 N2 - Quantitative (1)H-NMR ((1)H-qNMR) was applied to the determination of paeonol concentration in Moutan cortex, Hachimijiogan, and Keishibukuryogan. Paeonol is a major component of Moutan cortex, and its purity was calculated from the ratio of the intensity of the paeonol H-3' signal at δ 6.41 ppm in methanol-d 4 or 6.40 ppm in methanol-d 4 + TFA-d to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material grade. As a result, the paeonol content in two lots of Moutan cortex as determined by (1)H-qNMR was found to be 1.59 % and 1.62 %, respectively, while the paeonol content in Hachimijiogan and Keishibukuryogan was 0.15 % and 0.22 %, respectively. The present study demonstrated that the (1)H-NMR method is useful for the quantitative analysis of crude drugs and Kampo formulas. SN - 1861-0293 UR - https://www.unboundmedicine.com/medline/citation/27164909/Application_of_a_quantitative__1_H_NMR_method_for_the_determination_of_paeonol_in_Moutan_cortex_Hachimijiogan_and_Keishibukuryogan_ L2 - https://dx.doi.org/10.1007/s11418-016-1003-3 DB - PRIME DP - Unbound Medicine ER -