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Dietary Oil Source and Selenium Supplementation Modulate Fads2 and Elovl5 Transcriptional Levels in Liver and Brain of Meagre (Argyrosomus regius).
Lipids. 2016 06; 51(6):729-41.L

Abstract

The meagre (Argyrosomus regius) is taking on increasing importance in the aquaculture industry. In view of the limited supply of fish oil (FO) as a feed ingredient, the study of the capacity to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from alternative dietary oil sources is important. We analyzed changes in fatty acid (FA) desaturase 2 (fads2) and FA elongase 5 (elovl5) mRNA levels in livers and brains in response to FO replacement with a blend of vegetable oils (VO) and selenium (Se) supplementation. Fish were fed for 60 days with either a diet containing FO or a diet including VO, each supplemented or not with organic Se. Results showed that fads2 and elovl5 transcription was higher in liver when fish were fed VO diets. The brain mRNA levels of both genes were not affected by the dietary replacement of FO by VO. FA composition in the liver and skeletal muscle was altered by FO replacement, particularly by decreasing eicosapentaenoic acid and docosahexaenoic acid contents. The α-linolenic, linoleic, and arachidonic acid contents increased in both liver and brain of fish fed VO diets. The effect of Se supplementation on lipid metabolism was evident only in fish fed FO, showing a decrease in the transcription of hepatic fads2. Results indicate that the total replacement of FO by VO in diets modulates the expression of genes involved in LC-PUFA biosynthesis in meagre, affecting the FA profile of the fish flesh.

Authors+Show Affiliations

CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal. ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal. lmagnoni@ciimar.up.pt. IIB-INTECH - Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (CONICET), Chascomús, Argentina. lmagnoni@ciimar.up.pt.UFPB - Universidade Federal da Paraíba, Paraíba, Brazil. CCTA - Centro de Ciencias e Tecnologia Agroalimentar, UFCG - Universidade Federal de Campina Grande, Paraíba, Brazil.CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal. ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal. FCUP - Faculty of Sciences, University of Porto, Porto, Portugal.CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal.CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal. ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal. ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal. ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27169705

Citation

Silva-Brito, Francisca, et al. "Dietary Oil Source and Selenium Supplementation Modulate Fads2 and Elovl5 Transcriptional Levels in Liver and Brain of Meagre (Argyrosomus Regius)." Lipids, vol. 51, no. 6, 2016, pp. 729-41.
Silva-Brito F, Magnoni LJ, Fonseca SB, et al. Dietary Oil Source and Selenium Supplementation Modulate Fads2 and Elovl5 Transcriptional Levels in Liver and Brain of Meagre (Argyrosomus regius). Lipids. 2016;51(6):729-41.
Silva-Brito, F., Magnoni, L. J., Fonseca, S. B., Peixoto, M. J., Castro, L. F., Cunha, I., de Almeida Ozório, R. O., Magalhães, F. A., & Gonçalves, J. F. (2016). Dietary Oil Source and Selenium Supplementation Modulate Fads2 and Elovl5 Transcriptional Levels in Liver and Brain of Meagre (Argyrosomus regius). Lipids, 51(6), 729-41. https://doi.org/10.1007/s11745-016-4157-6
Silva-Brito F, et al. Dietary Oil Source and Selenium Supplementation Modulate Fads2 and Elovl5 Transcriptional Levels in Liver and Brain of Meagre (Argyrosomus Regius). Lipids. 2016;51(6):729-41. PubMed PMID: 27169705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary Oil Source and Selenium Supplementation Modulate Fads2 and Elovl5 Transcriptional Levels in Liver and Brain of Meagre (Argyrosomus regius). AU - Silva-Brito,Francisca, AU - Magnoni,Leonardo J, AU - Fonseca,Sthelio Braga, AU - Peixoto,Maria João, AU - Castro,L Filipe C, AU - Cunha,Isabel, AU - de Almeida Ozório,Rodrigo Otávio, AU - Magalhães,Fernando Antunes, AU - Gonçalves,José Fernando Magalhães, Y1 - 2016/05/12/ PY - 2015/11/19/received PY - 2016/04/19/accepted PY - 2016/5/13/entrez PY - 2016/5/14/pubmed PY - 2017/7/20/medline KW - Fatty acid desaturases KW - Fatty acid elongase KW - Fish oil replacement KW - Meagre SP - 729 EP - 41 JF - Lipids JO - Lipids VL - 51 IS - 6 N2 - The meagre (Argyrosomus regius) is taking on increasing importance in the aquaculture industry. In view of the limited supply of fish oil (FO) as a feed ingredient, the study of the capacity to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from alternative dietary oil sources is important. We analyzed changes in fatty acid (FA) desaturase 2 (fads2) and FA elongase 5 (elovl5) mRNA levels in livers and brains in response to FO replacement with a blend of vegetable oils (VO) and selenium (Se) supplementation. Fish were fed for 60 days with either a diet containing FO or a diet including VO, each supplemented or not with organic Se. Results showed that fads2 and elovl5 transcription was higher in liver when fish were fed VO diets. The brain mRNA levels of both genes were not affected by the dietary replacement of FO by VO. FA composition in the liver and skeletal muscle was altered by FO replacement, particularly by decreasing eicosapentaenoic acid and docosahexaenoic acid contents. The α-linolenic, linoleic, and arachidonic acid contents increased in both liver and brain of fish fed VO diets. The effect of Se supplementation on lipid metabolism was evident only in fish fed FO, showing a decrease in the transcription of hepatic fads2. Results indicate that the total replacement of FO by VO in diets modulates the expression of genes involved in LC-PUFA biosynthesis in meagre, affecting the FA profile of the fish flesh. SN - 1558-9307 UR - https://www.unboundmedicine.com/medline/citation/27169705/Dietary_Oil_Source_and_Selenium_Supplementation_Modulate_Fads2_and_Elovl5_Transcriptional_Levels_in_Liver_and_Brain_of_Meagre__Argyrosomus_regius__ L2 - https://link.springer.com/article/10.1007/s11745-016-4157-6 DB - PRIME DP - Unbound Medicine ER -