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MiR-195 participates in the placental disorder of preeclampsia via targeting activin receptor type-2B in trophoblastic cells.
J Hypertens 2016; 34(7):1371-9JH

Abstract

OBJECTIVE

Preeclampsia is a pregnancy-related syndrome. Shallow invasion of uterine wall by trophoblast cells has been generally accepted as the major pathological change of this disorder. We previously found downregulation of miR-195 in preeclamptic placentas. Bioinformatic analysis predicted a type II activin receptor, activin receptor type-2B (ActRIIB), as one of the potential targets of miR-195. Considering the key function of activin A on trophoblast cell behaviors and placenta development, we proposed miR-195 may affect trophoblast cell invasion by repressing the expression of ActRIIB.

METHODS

The colocalization of ActRIIB and miR-195 in human placenta was measured by in-situ hybridization and immunohistochemistry. Western blotting, real-time PCR and dual luciferase assay were performed in human trophoblast cell line, HTR8/SVneo cells, to validate the targeting of ActRIIB by miR-195. Cell invasiveness was analyzed using transwell insert invasion assay in HTR8/SVneo cells.

RESULTS

In human placenta, ActRIIB and miR-195 exhibited similar localization in various subtypes of trophoblast cells, including villous and extravillous trophoblasts. The protein expressions of ActRIIB in preeclamptic placenta were significantly higher as compared with the normal controls, which was opposite to the changing pattern of miR-195. In HTR8/SVneo cells, miR-195 could directly target and suppress the expression of ActRIIB. Meanwhile, the invasion-promoting effect of miR-195 on trophoblast cells could be largely impeded by ActRIIB overexpression.

CONCLUSION

In human trophoblast cells, miR-195 could promote cell invasion via directly targeting ActRIIB. The impaired miR-195 expression may contribute to the occurrence or development of preeclampsia through interfering with activin/nodal signaling in the placenta.

Authors+Show Affiliations

aDepartment of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou bDepartment of Obstetric and Gynecology, Aviation General Hospital of China Medical University and Beijing Institute of Translational Medicine cState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences dUniversity of Chinese Academy of Sciences, Beijing, China eDepartment of Biology, York University, Toronto, Ontario, Canada *HWu and HWang contributed equally to the writing of this article.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27176145

Citation

Wu, Hairong, et al. "MiR-195 Participates in the Placental Disorder of Preeclampsia Via Targeting Activin Receptor type-2B in Trophoblastic Cells." Journal of Hypertension, vol. 34, no. 7, 2016, pp. 1371-9.
Wu H, Wang H, Liu M, et al. MiR-195 participates in the placental disorder of preeclampsia via targeting activin receptor type-2B in trophoblastic cells. J Hypertens. 2016;34(7):1371-9.
Wu, H., Wang, H., Liu, M., Bai, Y., Li, Y. X., Ji, L., ... Wang, Y. L. (2016). MiR-195 participates in the placental disorder of preeclampsia via targeting activin receptor type-2B in trophoblastic cells. Journal of Hypertension, 34(7), pp. 1371-9. doi:10.1097/HJH.0000000000000948.
Wu H, et al. MiR-195 Participates in the Placental Disorder of Preeclampsia Via Targeting Activin Receptor type-2B in Trophoblastic Cells. J Hypertens. 2016;34(7):1371-9. PubMed PMID: 27176145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MiR-195 participates in the placental disorder of preeclampsia via targeting activin receptor type-2B in trophoblastic cells. AU - Wu,Hairong, AU - Wang,Hao, AU - Liu,Ming, AU - Bai,Yang, AU - Li,Yu-Xia, AU - Ji,Lei, AU - Peng,Chun, AU - Yu,Yanhong, AU - Wang,Yan-Ling, PY - 2016/5/14/entrez PY - 2016/5/14/pubmed PY - 2017/8/9/medline SP - 1371 EP - 9 JF - Journal of hypertension JO - J. Hypertens. VL - 34 IS - 7 N2 - OBJECTIVE: Preeclampsia is a pregnancy-related syndrome. Shallow invasion of uterine wall by trophoblast cells has been generally accepted as the major pathological change of this disorder. We previously found downregulation of miR-195 in preeclamptic placentas. Bioinformatic analysis predicted a type II activin receptor, activin receptor type-2B (ActRIIB), as one of the potential targets of miR-195. Considering the key function of activin A on trophoblast cell behaviors and placenta development, we proposed miR-195 may affect trophoblast cell invasion by repressing the expression of ActRIIB. METHODS: The colocalization of ActRIIB and miR-195 in human placenta was measured by in-situ hybridization and immunohistochemistry. Western blotting, real-time PCR and dual luciferase assay were performed in human trophoblast cell line, HTR8/SVneo cells, to validate the targeting of ActRIIB by miR-195. Cell invasiveness was analyzed using transwell insert invasion assay in HTR8/SVneo cells. RESULTS: In human placenta, ActRIIB and miR-195 exhibited similar localization in various subtypes of trophoblast cells, including villous and extravillous trophoblasts. The protein expressions of ActRIIB in preeclamptic placenta were significantly higher as compared with the normal controls, which was opposite to the changing pattern of miR-195. In HTR8/SVneo cells, miR-195 could directly target and suppress the expression of ActRIIB. Meanwhile, the invasion-promoting effect of miR-195 on trophoblast cells could be largely impeded by ActRIIB overexpression. CONCLUSION: In human trophoblast cells, miR-195 could promote cell invasion via directly targeting ActRIIB. The impaired miR-195 expression may contribute to the occurrence or development of preeclampsia through interfering with activin/nodal signaling in the placenta. SN - 1473-5598 UR - https://www.unboundmedicine.com/medline/citation/27176145/MiR_195_participates_in_the_placental_disorder_of_preeclampsia_via_targeting_activin_receptor_type_2B_in_trophoblastic_cells_ L2 - http://dx.doi.org/10.1097/HJH.0000000000000948 DB - PRIME DP - Unbound Medicine ER -