Tags

Type your tag names separated by a space and hit enter

Emulsified isoflurane treatment inhibits the cell cycle and respiration of human bronchial epithelial 16HBE cells in a p53-independent manner.
Mol Med Rep. 2016 Jul; 14(1):349-54.MM

Abstract

Emulsified isoflurane (EIso), as a result of its rapid anesthetic induction, recovery and convenience, is widely used as a novel intravenous general anesthetic. Treatment with EIso can reduce injuries caused by ischemia/reperfusion (I/R) to organs, including the heart, lung and liver, without knowing understanding the molecular mechanism. The present study hypothesized that treatment with EIso can affect the physiological processes of human lung bronchial epithelial cells (16HBE) prior to I/R. To test this hypothesis, the present study first constructed stable p53 knockdown and synthesis of cytochrome c oxidase (SCO)2 knockdown 16HBE cells. The above cells were subsequently treated with EIso at a concentration of 0.1 and 0.2% for 24 h. The relevant concentration of fat emulsion was used as a negative control. The expression levels of p53, p21, SCO1, SCO2 and Tp53‑induced glycolysis and apoptosis regulator (TIGAR) were detected by reverse transcription‑quantitative polymerase chain reaction and western blotting. Subsequently, the cell proliferation, respiration and glycolysis were investigated. The results revealed that EIso treatment significantly decreased the transcription of TIGAR, SCO1 and SCO2, and increased the transcription of p21, which are all p53 target genes, in a p53-independent manner. The cell cycle was inhibited by arresting cells at the G0/G1 phase. Respiration was reduced, which caused a decrease in oxygen consumption and the accumulation of lactate and reactive oxygen species. Taken together, EIso treatment inhibited the proliferation and respiration, and promoted glycolysis in 16HBE cells. This regulatory pathway may represent a protective mechanism of EIso treatment by inhibiting cell growth and decreasing the oxygen consumption from I/R.

Authors+Show Affiliations

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.Department of Anesthesiology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27176636

Citation

Yang, Hui, et al. "Emulsified Isoflurane Treatment Inhibits the Cell Cycle and Respiration of Human Bronchial Epithelial 16HBE Cells in a P53-independent Manner." Molecular Medicine Reports, vol. 14, no. 1, 2016, pp. 349-54.
Yang H, Deng J, Jiang Y, et al. Emulsified isoflurane treatment inhibits the cell cycle and respiration of human bronchial epithelial 16HBE cells in a p53-independent manner. Mol Med Rep. 2016;14(1):349-54.
Yang, H., Deng, J., Jiang, Y., Chen, J., Zeng, X., He, Z., Jiang, X., Li, Z., & Jiang, C. (2016). Emulsified isoflurane treatment inhibits the cell cycle and respiration of human bronchial epithelial 16HBE cells in a p53-independent manner. Molecular Medicine Reports, 14(1), 349-54. https://doi.org/10.3892/mmr.2016.5257
Yang H, et al. Emulsified Isoflurane Treatment Inhibits the Cell Cycle and Respiration of Human Bronchial Epithelial 16HBE Cells in a P53-independent Manner. Mol Med Rep. 2016;14(1):349-54. PubMed PMID: 27176636.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Emulsified isoflurane treatment inhibits the cell cycle and respiration of human bronchial epithelial 16HBE cells in a p53-independent manner. AU - Yang,Hui, AU - Deng,Jia, AU - Jiang,Yingying, AU - Chen,Jiao, AU - Zeng,Xianzheng, AU - He,Zhiyang, AU - Jiang,Xiaojuan, AU - Li,Zhuoning, AU - Jiang,Chunling, Y1 - 2016/05/12/ PY - 2015/06/02/received PY - 2016/04/14/accepted PY - 2016/5/14/entrez PY - 2016/5/14/pubmed PY - 2017/4/7/medline SP - 349 EP - 54 JF - Molecular medicine reports JO - Mol Med Rep VL - 14 IS - 1 N2 - Emulsified isoflurane (EIso), as a result of its rapid anesthetic induction, recovery and convenience, is widely used as a novel intravenous general anesthetic. Treatment with EIso can reduce injuries caused by ischemia/reperfusion (I/R) to organs, including the heart, lung and liver, without knowing understanding the molecular mechanism. The present study hypothesized that treatment with EIso can affect the physiological processes of human lung bronchial epithelial cells (16HBE) prior to I/R. To test this hypothesis, the present study first constructed stable p53 knockdown and synthesis of cytochrome c oxidase (SCO)2 knockdown 16HBE cells. The above cells were subsequently treated with EIso at a concentration of 0.1 and 0.2% for 24 h. The relevant concentration of fat emulsion was used as a negative control. The expression levels of p53, p21, SCO1, SCO2 and Tp53‑induced glycolysis and apoptosis regulator (TIGAR) were detected by reverse transcription‑quantitative polymerase chain reaction and western blotting. Subsequently, the cell proliferation, respiration and glycolysis were investigated. The results revealed that EIso treatment significantly decreased the transcription of TIGAR, SCO1 and SCO2, and increased the transcription of p21, which are all p53 target genes, in a p53-independent manner. The cell cycle was inhibited by arresting cells at the G0/G1 phase. Respiration was reduced, which caused a decrease in oxygen consumption and the accumulation of lactate and reactive oxygen species. Taken together, EIso treatment inhibited the proliferation and respiration, and promoted glycolysis in 16HBE cells. This regulatory pathway may represent a protective mechanism of EIso treatment by inhibiting cell growth and decreasing the oxygen consumption from I/R. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/27176636/Emulsified_isoflurane_treatment_inhibits_the_cell_cycle_and_respiration_of_human_bronchial_epithelial_16HBE_cells_in_a_p53_independent_manner_ L2 - http://www.spandidos-publications.com/mmr/14/1/349 DB - PRIME DP - Unbound Medicine ER -