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Divergent Development of Hexaploid Triticale by a Wheat - Rye -Psathyrostachys huashanica Trigeneric Hybrid Method.
PLoS One. 2016; 11(5):e0155667.Plos

Abstract

Hexaploid triticale is an important forage crop and a promising energy plant. Some forms were previously reported for developing the hexaploid triticale, such as crossing tetraploid wheat or hexaploid wheat with rye, crossing hexaploid triticale and/or hexaploid wheat with octoploid triticale, and spontaneously appearing in the selfed progenies of octoploid triticale. In the present study, we developed an effective method for production of diverse types of hexaploid triticale via wheat-rye-Psathyrostachys huashanica trigeneric hybrid. Genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) karyotyping revealed that D genome chromosomes were completely eliminated and the whole A, B, and R genome chromosomes were retained in three lines. More interestingly, the composite genome of the line K14-489-2 consisted of complete A and B genomes and chromosomes 1D, 2R, 3R, 4R, 5R, 6R, and 7R, that of line K14-491-2 was 12 A-genome (1A-6A), 14 B-genome (1B-7B), 12 R-genome (1R-3R, 5R-7R), and chromosomes 1D and 3D, and that of the line K14-547-1 had 26A/B and 14R chromosomes, plus one pair of centric 6BL/2DS translocations. This finding implies that some of D genome chromosomes can be spontaneously and stably incorporated into the hexaploid triticale. Additionally, a variety of high-molecular-weight glutenin subunits (HMW-GS) compositions were detected in the six hexaploid triticale lines, respectively. Besides, compared with its recurrent triticale parent Zhongsi828, these lines showed high level of resistance to stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens prevalent in China, including V26/Gui 22. These new hexaploid triticales not only enhanced diversification of triticale but also could be utilized as valuable germplasm for wheat improvement.

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Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Dazhou Institute of Agricultural Science, 188 Jianmin Road, Dazhou, 635000, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

College of Resources, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

MeSH

Chromosomes, PlantGenome, PlantHybridization, GeneticIn Situ Hybridization, FluorescenceMiosisPoaceaePolyploidySecaleTriticaleTriticum

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27182983

Citation

Kang, Houyang, et al. "Divergent Development of Hexaploid Triticale By a Wheat - Rye -Psathyrostachys Huashanica Trigeneric Hybrid Method." PloS One, vol. 11, no. 5, 2016, pp. e0155667.

Kang H, Wang H, Huang J, et al. Divergent Development of Hexaploid Triticale by a Wheat - Rye -Psathyrostachys huashanica Trigeneric Hybrid Method. PLoS One. 2016;11(5):e0155667.

Kang, H., Wang, H., Huang, J., Wang, Y., Li, D., Diao, C., Zhu, W., Tang, Y., Wang, Y., Fan, X., Zeng, J., Xu, L., Sha, L., Zhang, H., & Zhou, Y. (2016). Divergent Development of Hexaploid Triticale by a Wheat - Rye -Psathyrostachys huashanica Trigeneric Hybrid Method. PloS One, 11(5), e0155667. https://doi.org/10.1371/journal.pone.0155667

Kang H, et al. Divergent Development of Hexaploid Triticale By a Wheat - Rye -Psathyrostachys Huashanica Trigeneric Hybrid Method. PLoS One. 2016;11(5):e0155667. PubMed PMID: 27182983.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Divergent Development of Hexaploid Triticale by a Wheat - Rye -Psathyrostachys huashanica Trigeneric Hybrid Method. AU - Kang,Houyang, AU - Wang,Hao, AU - Huang,Juan, AU - Wang,Yujie, AU - Li,Daiyan, AU - Diao,Chengdou, AU - Zhu,Wei, AU - Tang,Yao, AU - Wang,Yi, AU - Fan,Xing, AU - Zeng,Jian, AU - Xu,Lili, AU - Sha,Lina, AU - Zhang,Haiqin, AU - Zhou,Yonghong, Y1 - 2016/05/16/ PY - 2016/01/14/received PY - 2016/05/01/accepted PY - 2016/5/17/entrez PY - 2016/5/18/pubmed PY - 2017/7/8/medline SP - e0155667 EP - e0155667 JF - PloS one JO - PLoS One VL - 11 IS - 5 N2 - Hexaploid triticale is an important forage crop and a promising energy plant. Some forms were previously reported for developing the hexaploid triticale, such as crossing tetraploid wheat or hexaploid wheat with rye, crossing hexaploid triticale and/or hexaploid wheat with octoploid triticale, and spontaneously appearing in the selfed progenies of octoploid triticale. In the present study, we developed an effective method for production of diverse types of hexaploid triticale via wheat-rye-Psathyrostachys huashanica trigeneric hybrid. Genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) karyotyping revealed that D genome chromosomes were completely eliminated and the whole A, B, and R genome chromosomes were retained in three lines. More interestingly, the composite genome of the line K14-489-2 consisted of complete A and B genomes and chromosomes 1D, 2R, 3R, 4R, 5R, 6R, and 7R, that of line K14-491-2 was 12 A-genome (1A-6A), 14 B-genome (1B-7B), 12 R-genome (1R-3R, 5R-7R), and chromosomes 1D and 3D, and that of the line K14-547-1 had 26A/B and 14R chromosomes, plus one pair of centric 6BL/2DS translocations. This finding implies that some of D genome chromosomes can be spontaneously and stably incorporated into the hexaploid triticale. Additionally, a variety of high-molecular-weight glutenin subunits (HMW-GS) compositions were detected in the six hexaploid triticale lines, respectively. Besides, compared with its recurrent triticale parent Zhongsi828, these lines showed high level of resistance to stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens prevalent in China, including V26/Gui 22. These new hexaploid triticales not only enhanced diversification of triticale but also could be utilized as valuable germplasm for wheat improvement. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27182983/Divergent_Development_of_Hexaploid_Triticale_by_a_Wheat___Rye__Psathyrostachys_huashanica_Trigeneric_Hybrid_Method_ DB - PRIME DP - Unbound Medicine ER -