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Characterization of the genetic environment of the blaKPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital.
Braz J Infect Dis. 2016 Jul-Aug; 20(4):384-8.BJ

Abstract

Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of blaKPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shanghai, China. Forty-two clinical isolates were included in this study by blaKPC-2 screening. After multilocus sequence typing and plasmid analyses of PCR-based replicon typing (PBRT), junction PCR, mapping PCR and crossing PCR assays, primer walking, and amplicon sequencing were used to analyze the genetic environment of the blaKPC-2 gene. ST423, ST65, ST977, and ST11 were all detected in KPC-2-producing K. pneumoniae. Two types of blaKPC-2-bearing genetic structure were found: Tn1721-blaKPC-2-Tn3 and Tn1721-blaKPC-2-ΔTn3-IS26; and were carried in IncX and IncFII plasmids, respectively. In conclusion, the genetic environment of the blaKPC-2 gene was diverse and Tn1721-blaKPC-2-ΔTn3-IS26 was dominant in clinical K. pneumoniae isolates in Huashan Hospital. This study sheds some light on the genetic environment and should foster further studies about the mechanism of the blaKPC-2 dissemination.

Authors+Show Affiliations

Fudan University, Shanghai Medical College, Huashan Hospital, Department of Laboratory Medicine, Shanghai, China.Fudan University, Shanghai Medical College, Huashan Hospital, Department of Laboratory Medicine, Shanghai, China; Hospital of Fudan University, Department of Laboratory Medicine, Obstetrics and Gynecology, Shanghai, China.Fudan University, Shanghai Medical College, Huashan Hospital, Department of Laboratory Medicine, Shanghai, China; Fudan University, Shanghai Medical College, Jinshan Hospital, Department of Laboratory Medicine, Shanghai, China.Fudan University, Shanghai Medical College, Huashan Hospital, Department of Laboratory Medicine, Shanghai, China. Electronic address: jiangxi2154@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27183358

Citation

Shen, Pinghua, et al. "Characterization of the Genetic Environment of the blaKPC-2 Gene Among Klebsiella Pneumoniae Isolates From a Chinese Hospital." The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases, vol. 20, no. 4, 2016, pp. 384-8.
Shen P, Zhang Y, Li G, et al. Characterization of the genetic environment of the blaKPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital. Braz J Infect Dis. 2016;20(4):384-8.
Shen, P., Zhang, Y., Li, G., & Jiang, X. (2016). Characterization of the genetic environment of the blaKPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital. The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases, 20(4), 384-8. https://doi.org/10.1016/j.bjid.2016.04.003
Shen P, et al. Characterization of the Genetic Environment of the blaKPC-2 Gene Among Klebsiella Pneumoniae Isolates From a Chinese Hospital. Braz J Infect Dis. 2016 Jul-Aug;20(4):384-8. PubMed PMID: 27183358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the genetic environment of the blaKPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital. AU - Shen,Pinghua, AU - Zhang,Ying, AU - Li,Gang, AU - Jiang,Xiaofei, Y1 - 2016/05/13/ PY - 2015/12/14/received PY - 2016/04/19/revised PY - 2016/04/19/accepted PY - 2016/5/17/entrez PY - 2016/5/18/pubmed PY - 2017/3/3/medline KW - Genetic environment KW - Klebsiella pneumoniae KW - bla(KPC) SP - 384 EP - 8 JF - The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases JO - Braz J Infect Dis VL - 20 IS - 4 N2 - Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of blaKPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shanghai, China. Forty-two clinical isolates were included in this study by blaKPC-2 screening. After multilocus sequence typing and plasmid analyses of PCR-based replicon typing (PBRT), junction PCR, mapping PCR and crossing PCR assays, primer walking, and amplicon sequencing were used to analyze the genetic environment of the blaKPC-2 gene. ST423, ST65, ST977, and ST11 were all detected in KPC-2-producing K. pneumoniae. Two types of blaKPC-2-bearing genetic structure were found: Tn1721-blaKPC-2-Tn3 and Tn1721-blaKPC-2-ΔTn3-IS26; and were carried in IncX and IncFII plasmids, respectively. In conclusion, the genetic environment of the blaKPC-2 gene was diverse and Tn1721-blaKPC-2-ΔTn3-IS26 was dominant in clinical K. pneumoniae isolates in Huashan Hospital. This study sheds some light on the genetic environment and should foster further studies about the mechanism of the blaKPC-2 dissemination. SN - 1678-4391 UR - https://www.unboundmedicine.com/medline/citation/27183358/Characterization_of_the_genetic_environment_of_the_blaKPC_2_gene_among_Klebsiella_pneumoniae_isolates_from_a_Chinese_Hospital_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1413-8670(16)30073-3 DB - PRIME DP - Unbound Medicine ER -