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Pheretima aspergillum decoction suppresses inflammation and relieves asthma in a mouse model of bronchial asthma by NF-κB inhibition.
J Ethnopharmacol 2016; 189:22-30JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE AND AIM OF THE STUDY

Guang-Pheretima, the live form of the earthworm Pheretima aspergillum, is a traditional Chinese medicine commonly used for the treatment of asthma, cough, stroke, epilepsy and other diseases due to its anti-inflammatory, anti-asthmatic, anti-seizure, thrombolytic and diuretic properties. Although Guang-Pheretima is effective in the relief of asthma, its pharmacological activity and the underlying molecular mechanisms are not fully understood. Hence, we investigated the effects of a Pheretima aspergillum decoction (PAD) against inflammation in a model of ovalbumin (OVA)-induced asthma in BALB/c mice, as well as the nuclear factor-κB (NF-κB) pathway involved in this process.

MATERIALS AND METHODS

OVA was used to sensitize and challenge the airway of the mice, and PAD was administrated by gavage. We measured airway hyperresponsiveness (AHR) in the mice 24h following a final methacholine challenge with whole-body plethysmography. The bronchoalveolar lavage fluid (BALF), serum and pulmonary tissues were collected 48h after the last challenge. The levels of inflammatory factors and the related mRNAs were determined by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR), respectively. The number of differential inflammatory cells in the BALF was counted. Serum total and OVA-specific IgE levels were measured with ELISA. The activation of NF-κB signaling in the lung was detected by western blotting. In addition, the lung tissues were stained with hematoxylin and eosin or periodic acid Schiff stain for histopathological examination.

RESULTS

PAD treatment significantly alleviated AHR in the asthmatic mice, decreased the mRNA and protein levels of IL-4, IL-5 and IL-13 and downregulated IgE. In addition, PAD treatment attenuated mucus secretion and infiltration of inflammatory cells in the lung while inhibiting the activation of NF-κB signaling.

CONCLUSIONS

PAD effectively inhibited the activation of NF-κB signaling in the lungs of mice with OVA-induced asthma, and mitigated AHR and Th2 type inflammatory reactions. Therefore, PAD may serve as a drug candidate for asthma treatment.

Authors+Show Affiliations

School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. Electronic address: liwei-li@163.com.School of Pharmacy, Guangzhou Medical University, Guangzhou 511436, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27184188

Citation

Huang, Chuan-Qi, et al. "Pheretima Aspergillum Decoction Suppresses Inflammation and Relieves Asthma in a Mouse Model of Bronchial Asthma By NF-κB Inhibition." Journal of Ethnopharmacology, vol. 189, 2016, pp. 22-30.
Huang CQ, Li W, Wu B, et al. Pheretima aspergillum decoction suppresses inflammation and relieves asthma in a mouse model of bronchial asthma by NF-κB inhibition. J Ethnopharmacol. 2016;189:22-30.
Huang, C. Q., Li, W., Wu, B., Chen, W. M., Chen, L. H., Mo, G. W., ... Zeng, Q. Z. (2016). Pheretima aspergillum decoction suppresses inflammation and relieves asthma in a mouse model of bronchial asthma by NF-κB inhibition. Journal of Ethnopharmacology, 189, pp. 22-30. doi:10.1016/j.jep.2016.05.028.
Huang CQ, et al. Pheretima Aspergillum Decoction Suppresses Inflammation and Relieves Asthma in a Mouse Model of Bronchial Asthma By NF-κB Inhibition. J Ethnopharmacol. 2016 Aug 2;189:22-30. PubMed PMID: 27184188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pheretima aspergillum decoction suppresses inflammation and relieves asthma in a mouse model of bronchial asthma by NF-κB inhibition. AU - Huang,Chuan-Qi, AU - Li,Wei, AU - Wu,Bo, AU - Chen,Wei-Ming, AU - Chen,Li-Hong, AU - Mo,Guan-Wen, AU - Zhang,Qiu-Feng, AU - Gong,Ling, AU - Li,Jing, AU - Zhang,Hong-Chao, AU - Zhu,Hai-Mei, AU - Zeng,Qing-Zhong, Y1 - 2016/05/13/ PY - 2015/08/07/received PY - 2016/02/19/revised PY - 2016/05/11/accepted PY - 2016/5/18/entrez PY - 2016/5/18/pubmed PY - 2017/4/21/medline KW - Airway KW - Asthma KW - Choline (PubChem CID: 305) KW - Earthworm KW - Hypoxanthine (PubChem CID: 790) KW - OVA KW - Traditional Chinese medicine KW - l-alanine (PubChem CID: 5950) KW - l-leucine (PubChem CID: 6106) KW - l-lysine (PubChem CID: 5962) KW - l-valine (PubChem CID: 6287) SP - 22 EP - 30 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 189 N2 - ETHNOPHARMACOLOGICAL RELEVANCE AND AIM OF THE STUDY: Guang-Pheretima, the live form of the earthworm Pheretima aspergillum, is a traditional Chinese medicine commonly used for the treatment of asthma, cough, stroke, epilepsy and other diseases due to its anti-inflammatory, anti-asthmatic, anti-seizure, thrombolytic and diuretic properties. Although Guang-Pheretima is effective in the relief of asthma, its pharmacological activity and the underlying molecular mechanisms are not fully understood. Hence, we investigated the effects of a Pheretima aspergillum decoction (PAD) against inflammation in a model of ovalbumin (OVA)-induced asthma in BALB/c mice, as well as the nuclear factor-κB (NF-κB) pathway involved in this process. MATERIALS AND METHODS: OVA was used to sensitize and challenge the airway of the mice, and PAD was administrated by gavage. We measured airway hyperresponsiveness (AHR) in the mice 24h following a final methacholine challenge with whole-body plethysmography. The bronchoalveolar lavage fluid (BALF), serum and pulmonary tissues were collected 48h after the last challenge. The levels of inflammatory factors and the related mRNAs were determined by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR), respectively. The number of differential inflammatory cells in the BALF was counted. Serum total and OVA-specific IgE levels were measured with ELISA. The activation of NF-κB signaling in the lung was detected by western blotting. In addition, the lung tissues were stained with hematoxylin and eosin or periodic acid Schiff stain for histopathological examination. RESULTS: PAD treatment significantly alleviated AHR in the asthmatic mice, decreased the mRNA and protein levels of IL-4, IL-5 and IL-13 and downregulated IgE. In addition, PAD treatment attenuated mucus secretion and infiltration of inflammatory cells in the lung while inhibiting the activation of NF-κB signaling. CONCLUSIONS: PAD effectively inhibited the activation of NF-κB signaling in the lungs of mice with OVA-induced asthma, and mitigated AHR and Th2 type inflammatory reactions. Therefore, PAD may serve as a drug candidate for asthma treatment. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27184188/Pheretima_aspergillum_decoction_suppresses_inflammation_and_relieves_asthma_in_a_mouse_model_of_bronchial_asthma_by_NF_κB_inhibition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30300-2 DB - PRIME DP - Unbound Medicine ER -