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The WRKY Transcription Factor Family in Citrus: Valuable and Useful Candidate Genes for Citrus Breeding.
Appl Biochem Biotechnol. 2016 Oct; 180(3):516-543.AB

Abstract

WRKY transcription factors belong to a large family of plant transcriptional regulators whose members have been reported to be involved in a wide range of biological roles including plant development, adaptation to environmental constraints and response to several diseases. However, little or poor information is available about WRKY's in Citrus. The recent release of completely assembled genomes sequences of Citrus sinensis and Citrus clementina and the availability of ESTs sequences from other citrus species allowed us to perform a genome survey for Citrus WRKY proteins. In the present study, we identified 100 WRKY members from C. sinensis (51), C. clementina (48) and Citrus unshiu (1), and analyzed their chromosomal distribution, gene structure, gene duplication, syntenic relation and phylogenetic analysis. A phylogenetic tree of 100 Citrus WRKY sequences with their orthologs from Arabidopsis has distinguished seven groups. The CsWRKY genes were distributed across all ten sweet orange chromosomes. A comprehensive approach and an integrative analysis of Citrus WRKY gene expression revealed variable profiles of expression within tissues and stress conditions indicating functional diversification. Thus, candidate Citrus WRKY genes have been proposed as potentially involved in fruit acidification, essential oil biosynthesis and abiotic/biotic stress tolerance. Our results provided essential prerequisites for further WRKY genes cloning and functional analysis with an aim of citrus crop improvement.

Authors+Show Affiliations

Laboratory of Extremophile Plants. Center of Biotechnology of Borj-Cédria (CBBC), BP 901, Hammam-lif, 2050, Tunisia. mariem.ayedi@gmail.com. Laboratory of Molecular and Cellular Screening Processes, Center of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road, P.O. Box 1177, 3018, Sfax, Tunisia. mariem.ayedi@gmail.com.Laboratory of Extremophile Plants. Center of Biotechnology of Borj-Cédria (CBBC), BP 901, Hammam-lif, 2050, Tunisia.Laboratory of Molecular and Cellular Screening Processes, Center of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road, P.O. Box 1177, 3018, Sfax, Tunisia.Laboratory of Extremophile Plants. Center of Biotechnology of Borj-Cédria (CBBC), BP 901, Hammam-lif, 2050, Tunisia.Laboratory of Molecular and Cellular Screening Processes, Center of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road, P.O. Box 1177, 3018, Sfax, Tunisia.Unit 'Ecophysiology and Grape Functional Genomics' Institute of Vine and Wine Sciences, INRA Bordeaux-Aquitaine, 210 Chemin de Leysotte - CS 50008, 33882, Villenave d'Ornon Cedex, France.Laboratory of Molecular and Cellular Screening Processes, Center of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road, P.O. Box 1177, 3018, Sfax, Tunisia.Laboratory of Extremophile Plants. Center of Biotechnology of Borj-Cédria (CBBC), BP 901, Hammam-lif, 2050, Tunisia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27193354

Citation

Ayadi, M, et al. "The WRKY Transcription Factor Family in Citrus: Valuable and Useful Candidate Genes for Citrus Breeding." Applied Biochemistry and Biotechnology, vol. 180, no. 3, 2016, pp. 516-543.
Ayadi M, Hanana M, Kharrat N, et al. The WRKY Transcription Factor Family in Citrus: Valuable and Useful Candidate Genes for Citrus Breeding. Appl Biochem Biotechnol. 2016;180(3):516-543.
Ayadi, M., Hanana, M., Kharrat, N., Merchaoui, H., Marzoug, R. B., Lauvergeat, V., Rebaï, A., & Mzid, R. (2016). The WRKY Transcription Factor Family in Citrus: Valuable and Useful Candidate Genes for Citrus Breeding. Applied Biochemistry and Biotechnology, 180(3), 516-543.
Ayadi M, et al. The WRKY Transcription Factor Family in Citrus: Valuable and Useful Candidate Genes for Citrus Breeding. Appl Biochem Biotechnol. 2016;180(3):516-543. PubMed PMID: 27193354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The WRKY Transcription Factor Family in Citrus: Valuable and Useful Candidate Genes for Citrus Breeding. AU - Ayadi,M, AU - Hanana,M, AU - Kharrat,N, AU - Merchaoui,H, AU - Marzoug,R Ben, AU - Lauvergeat,V, AU - Rebaï,A, AU - Mzid,R, Y1 - 2016/05/19/ PY - 2015/10/16/received PY - 2016/05/05/accepted PY - 2016/5/20/pubmed PY - 2017/1/25/medline PY - 2016/5/20/entrez KW - Citrus KW - Essential oil biosynthesis KW - Fruit acidification KW - Stress tolerance KW - WRKY KW - Zinc finger SP - 516 EP - 543 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 180 IS - 3 N2 - WRKY transcription factors belong to a large family of plant transcriptional regulators whose members have been reported to be involved in a wide range of biological roles including plant development, adaptation to environmental constraints and response to several diseases. However, little or poor information is available about WRKY's in Citrus. The recent release of completely assembled genomes sequences of Citrus sinensis and Citrus clementina and the availability of ESTs sequences from other citrus species allowed us to perform a genome survey for Citrus WRKY proteins. In the present study, we identified 100 WRKY members from C. sinensis (51), C. clementina (48) and Citrus unshiu (1), and analyzed their chromosomal distribution, gene structure, gene duplication, syntenic relation and phylogenetic analysis. A phylogenetic tree of 100 Citrus WRKY sequences with their orthologs from Arabidopsis has distinguished seven groups. The CsWRKY genes were distributed across all ten sweet orange chromosomes. A comprehensive approach and an integrative analysis of Citrus WRKY gene expression revealed variable profiles of expression within tissues and stress conditions indicating functional diversification. Thus, candidate Citrus WRKY genes have been proposed as potentially involved in fruit acidification, essential oil biosynthesis and abiotic/biotic stress tolerance. Our results provided essential prerequisites for further WRKY genes cloning and functional analysis with an aim of citrus crop improvement. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/27193354/The_WRKY_Transcription_Factor_Family_in_Citrus:_Valuable_and_Useful_Candidate_Genes_for_Citrus_Breeding_ DB - PRIME DP - Unbound Medicine ER -