Tags

Type your tag names separated by a space and hit enter

A resistance locus in the American heirloom rice variety Carolina Gold Select is triggered by TAL effectors with diverse predicted targets and is effective against African strains of Xanthomonas oryzae pv. oryzicola.
Plant J. 2016 09; 87(5):472-83.PJ

Abstract

The rice pathogens Xanthomonas oryzae pathovar (pv.) oryzae and pv. oryzicola produce numerous transcription activator-like (TAL) effectors that increase bacterial virulence by activating expression of host susceptibility genes. Rice resistance mechanisms against TAL effectors include polymorphisms that prevent effector binding to susceptibility gene promoters, or that allow effector activation of resistance genes. This study identifies, in the heirloom variety Carolina Gold Select, a third mechanism of rice resistance involving TAL effectors. This resistance manifests through strong suppression of disease development in response to diverse TAL effectors from both X. oryzae pathovars. The resistance can be triggered by an effector with only 3.5 central repeats, is independent of the composition of the repeat variable di-residues that determine TAL effector binding specificity, and is independent of the transcriptional activation domain. We determined that the resistance is conferred by a single dominant locus, designated Xo1, that maps to a 1.09 Mbp fragment on chromosome 4. The Xo1 interval also confers complete resistance to the strains in the African clade of X. oryzae pv. oryzicola, representing the first dominant resistance locus against bacterial leaf streak in rice. The strong phenotypic similarity between the TAL effector-triggered resistance conferred by Xo1 and that conferred by the tomato resistance gene Bs4 suggests that monocots and dicots share an ancient or convergently evolved mechanism to recognize analogous TAL effector epitopes.

Authors+Show Affiliations

Department of Plant Pathology and Ecology, The Connecticut Agricultural Experiment Station, New Haven, CT, 06511, USA. lindsay.triplett@CT.gov. Department of Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA. lindsay.triplett@CT.gov.Department of Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA.Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, 06520, USA.Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, 50011, USA.Department of Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA.Université des Sciences Techniques et Technologiques, Faculté des Sciences et Techniques, LBMA, Bamako, Mali.IRD, Cirad, Univ. Montpellier, Interactions Plantes Microorganismes Environnement (IPME), Montpellier, 34394, France.Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, 50011, USA. Plant Pathology and Plant Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.Department of Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27197779

Citation

Triplett, Lindsay R., et al. "A Resistance Locus in the American Heirloom Rice Variety Carolina Gold Select Is Triggered By TAL Effectors With Diverse Predicted Targets and Is Effective Against African Strains of Xanthomonas Oryzae Pv. Oryzicola." The Plant Journal : for Cell and Molecular Biology, vol. 87, no. 5, 2016, pp. 472-83.
Triplett LR, Cohen SP, Heffelfinger C, et al. A resistance locus in the American heirloom rice variety Carolina Gold Select is triggered by TAL effectors with diverse predicted targets and is effective against African strains of Xanthomonas oryzae pv. oryzicola. Plant J. 2016;87(5):472-83.
Triplett, L. R., Cohen, S. P., Heffelfinger, C., Schmidt, C. L., Huerta, A. I., Tekete, C., Verdier, V., Bogdanove, A. J., & Leach, J. E. (2016). A resistance locus in the American heirloom rice variety Carolina Gold Select is triggered by TAL effectors with diverse predicted targets and is effective against African strains of Xanthomonas oryzae pv. oryzicola. The Plant Journal : for Cell and Molecular Biology, 87(5), 472-83. https://doi.org/10.1111/tpj.13212
Triplett LR, et al. A Resistance Locus in the American Heirloom Rice Variety Carolina Gold Select Is Triggered By TAL Effectors With Diverse Predicted Targets and Is Effective Against African Strains of Xanthomonas Oryzae Pv. Oryzicola. Plant J. 2016;87(5):472-83. PubMed PMID: 27197779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A resistance locus in the American heirloom rice variety Carolina Gold Select is triggered by TAL effectors with diverse predicted targets and is effective against African strains of Xanthomonas oryzae pv. oryzicola. AU - Triplett,Lindsay R, AU - Cohen,Stephen P, AU - Heffelfinger,Christopher, AU - Schmidt,Clarice L, AU - Huerta,Alejandra I, AU - Tekete,Cheick, AU - Verdier,Valerie, AU - Bogdanove,Adam J, AU - Leach,Jan E, Y1 - 2016/08/02/ PY - 2015/09/05/received PY - 2016/05/11/revised PY - 2016/05/17/accepted PY - 2016/5/21/entrez PY - 2016/5/21/pubmed PY - 2017/11/8/medline KW - Bs4 KW - Carolina Gold Select KW - Oryza sativa KW - TAL effector KW - Xanthomonas oryzae KW - bacterial leaf blight KW - bacterial leaf streak SP - 472 EP - 83 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 87 IS - 5 N2 - The rice pathogens Xanthomonas oryzae pathovar (pv.) oryzae and pv. oryzicola produce numerous transcription activator-like (TAL) effectors that increase bacterial virulence by activating expression of host susceptibility genes. Rice resistance mechanisms against TAL effectors include polymorphisms that prevent effector binding to susceptibility gene promoters, or that allow effector activation of resistance genes. This study identifies, in the heirloom variety Carolina Gold Select, a third mechanism of rice resistance involving TAL effectors. This resistance manifests through strong suppression of disease development in response to diverse TAL effectors from both X. oryzae pathovars. The resistance can be triggered by an effector with only 3.5 central repeats, is independent of the composition of the repeat variable di-residues that determine TAL effector binding specificity, and is independent of the transcriptional activation domain. We determined that the resistance is conferred by a single dominant locus, designated Xo1, that maps to a 1.09 Mbp fragment on chromosome 4. The Xo1 interval also confers complete resistance to the strains in the African clade of X. oryzae pv. oryzicola, representing the first dominant resistance locus against bacterial leaf streak in rice. The strong phenotypic similarity between the TAL effector-triggered resistance conferred by Xo1 and that conferred by the tomato resistance gene Bs4 suggests that monocots and dicots share an ancient or convergently evolved mechanism to recognize analogous TAL effector epitopes. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/27197779/A_resistance_locus_in_the_American_heirloom_rice_variety_Carolina_Gold_Select_is_triggered_by_TAL_effectors_with_diverse_predicted_targets_and_is_effective_against_African_strains_of_Xanthomonas_oryzae_pv__oryzicola_ L2 - https://doi.org/10.1111/tpj.13212 DB - PRIME DP - Unbound Medicine ER -