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Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties.
Int J Nanomedicine. 2016; 11:1835-46.IJ

Abstract

A rapid phytosynthesis of silver nanoparticles (AgNPs) using an extract from the aerial parts of Artemisia marschalliana Sprengel was investigated in this study. The synthesized AgNPs using A. marschalliana extract was analyzed by UV-visible spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy and further characterized by transmission electron microscopy, scanning electron microscopy, zeta potential, and energy-dispersive spectroscopy. Characteristic absorption bands of AgNPs were found near 430 nm in the UV-vis spectrum. Energy-dispersive spectroscopy analysis of AgNPs in the energy range 2-4 keV confirmed the silver signal due to surface plasmon resonance. Scanning electron microscopy and transmission electron microscopy results revealed that the AgNPs were mostly spherical with an average size ranging from 5 nm to 50 nm. The zeta potential value of -31 mV confirmed the stability of the AgNPs. AgNPs produced using the aqueous A. marschalliana extract might serve as a potent in vitro antioxidant, as revealed by 2,2-diphenyl-1-picryl hydrazyl assay. The present study demonstrates the anticancer properties of phytosynthesized AgNPs against human gastric carcinoma AGS cells. AgNPs exerted a dose-dependent inhibitory effect on the viability of cells. Real-time polymerase chain reaction was used for the investigation of Bax and Bcl-2 gene expression in cancer and normal cell lines. Our findings show that the mRNA levels of pro-apoptotic Bax gene expression were significantly upregulated, while the expression of anti-apoptotic Bcl-2 was declined in cells treated with AgNPs compared to normal cells. In addition, flow cytometric analysis showed that the number of early and late apoptotic AGS cells was significantly enhanced following treatment with AgNPs as compared to untreated cells. In addition, the AgNPs showed strong antibacterial properties against tested pathogenic bacteria such as Staphylococcus aureus, Bacillus cereus, Acinetobacter baumannii, and Pseudomonas aeruginosa. Based on the obtained data, we suggest that phytosynthesized AgNPs are good alternatives in the treatment of diseases because of the presence of bioactive agents.

Authors+Show Affiliations

Department of Phytochemistry and Essential Oils Technology, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran (IAUPS), Iran.Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran.Department of Biology, Tehran North Branch, Iran.Department of Chemistry, Shahre-Rey Branch, Islamic Azad University, Tehran, Iran.Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran.Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27199558

Citation

Salehi, Soheil, et al. "Phytosynthesis of Silver Nanoparticles Using Artemisia Marschalliana Sprengel Aerial Part Extract and Assessment of Their Antioxidant, Anticancer, and Antibacterial Properties." International Journal of Nanomedicine, vol. 11, 2016, pp. 1835-46.
Salehi S, Shandiz SA, Ghanbar F, et al. Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. Int J Nanomedicine. 2016;11:1835-46.
Salehi, S., Shandiz, S. A., Ghanbar, F., Darvish, M. R., Ardestani, M. S., Mirzaie, A., & Jafari, M. (2016). Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. International Journal of Nanomedicine, 11, 1835-46. https://doi.org/10.2147/IJN.S99882
Salehi S, et al. Phytosynthesis of Silver Nanoparticles Using Artemisia Marschalliana Sprengel Aerial Part Extract and Assessment of Their Antioxidant, Anticancer, and Antibacterial Properties. Int J Nanomedicine. 2016;11:1835-46. PubMed PMID: 27199558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. AU - Salehi,Soheil, AU - Shandiz,Seyed Ataollah Sadat, AU - Ghanbar,Farinaz, AU - Darvish,Mohammad Raouf, AU - Ardestani,Mehdi Shafiee, AU - Mirzaie,Amir, AU - Jafari,Mohsen, Y1 - 2016/04/29/ PY - 2016/5/21/entrez PY - 2016/5/21/pubmed PY - 2017/2/28/medline KW - Artemisia marschalliana Sprengel KW - antibacterial KW - anticancer KW - silver nanoparticles SP - 1835 EP - 46 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 11 N2 - A rapid phytosynthesis of silver nanoparticles (AgNPs) using an extract from the aerial parts of Artemisia marschalliana Sprengel was investigated in this study. The synthesized AgNPs using A. marschalliana extract was analyzed by UV-visible spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy and further characterized by transmission electron microscopy, scanning electron microscopy, zeta potential, and energy-dispersive spectroscopy. Characteristic absorption bands of AgNPs were found near 430 nm in the UV-vis spectrum. Energy-dispersive spectroscopy analysis of AgNPs in the energy range 2-4 keV confirmed the silver signal due to surface plasmon resonance. Scanning electron microscopy and transmission electron microscopy results revealed that the AgNPs were mostly spherical with an average size ranging from 5 nm to 50 nm. The zeta potential value of -31 mV confirmed the stability of the AgNPs. AgNPs produced using the aqueous A. marschalliana extract might serve as a potent in vitro antioxidant, as revealed by 2,2-diphenyl-1-picryl hydrazyl assay. The present study demonstrates the anticancer properties of phytosynthesized AgNPs against human gastric carcinoma AGS cells. AgNPs exerted a dose-dependent inhibitory effect on the viability of cells. Real-time polymerase chain reaction was used for the investigation of Bax and Bcl-2 gene expression in cancer and normal cell lines. Our findings show that the mRNA levels of pro-apoptotic Bax gene expression were significantly upregulated, while the expression of anti-apoptotic Bcl-2 was declined in cells treated with AgNPs compared to normal cells. In addition, flow cytometric analysis showed that the number of early and late apoptotic AGS cells was significantly enhanced following treatment with AgNPs as compared to untreated cells. In addition, the AgNPs showed strong antibacterial properties against tested pathogenic bacteria such as Staphylococcus aureus, Bacillus cereus, Acinetobacter baumannii, and Pseudomonas aeruginosa. Based on the obtained data, we suggest that phytosynthesized AgNPs are good alternatives in the treatment of diseases because of the presence of bioactive agents. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/27199558/Phytosynthesis_of_silver_nanoparticles_using_Artemisia_marschalliana_Sprengel_aerial_part_extract_and_assessment_of_their_antioxidant_anticancer_and_antibacterial_properties_ L2 - https://dx.doi.org/10.2147/IJN.S99882 DB - PRIME DP - Unbound Medicine ER -