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Apoptosis-mediated testicular alteration in Japanese quail (Coturnix coturnix japonica) in response to temporal phase relation of serotonergic and dopaminergic oscillations.
J Exp Biol. 2016 05 15; 219(Pt 10):1476-87.JE

Abstract

Reproductive performance of many avian species, including Japanese quail, is reported to be modulated by specific temporal phase relation of serotonergic and dopaminergic oscillations. Accordingly, it has been shown that the serotonin precursor 5-HTP and the dopamine precursor l-DOPA given 8 h apart induce gonadal suppression and given 12 h apart lead to gonadal stimulation, while other temporal relationships were found to be ineffective. In the present study, we investigated the effects of 8- and 12-h phase relation of neural oscillations on testicular responses including expression of GnRH-I, GnIH, pro-apoptotic proteins (p53 and Bax), inactive and active executioner caspase-3, and the uncleaved DNA repair enzyme PARP-1. Testicular volume and mass decreased significantly in 8-h quail and increased in 12-h quail compared with controls. Expression of ir-GnIH, p53, Bax and active-caspase-3 increased and that of GnRH-I, pro-caspase-3 and uncleaved PARP-1 decreased in 8-h quail compared with controls. A TUNEL assay also confirmed testicular regression in these quail. Testes of 12-h quail exhibited significantly increased expression of GnRH-I, pro-caspase-3 and uncleaved PARP-1 compared with the control group. Our findings suggest that differential response of avian testes to 8- and 12-h phase relation of serotonergic and dopaminergic neural oscillations may be attributed to autocrine/paracrine action of GnIH expression, which is upregulated in regressed testes, leading to apoptotic changes, and downregulated in developed testes, causing apoptotic inhibition. It is concluded that specific phase relation of neural oscillations may modulate the local testicular GnRH-GnIH system and alter the apoptotic mechanism in quail testes. Moreover, these findings highlight the physiological effects of time-dependent drug delivery, including the specific time intervals between two drugs.

Authors+Show Affiliations

Department of Zoology, Banaras Hindu University, Varanasi 221005, India.Department of Biology, Waseda University, Tokyo 162-8480, Japan.Department of Zoology, Banaras Hindu University, Varanasi 221005, India cmchaturvedi@bhu.ac.in.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27207953

Citation

Banerjee, Somanshu, et al. "Apoptosis-mediated Testicular Alteration in Japanese Quail (Coturnix Coturnix Japonica) in Response to Temporal Phase Relation of Serotonergic and Dopaminergic Oscillations." The Journal of Experimental Biology, vol. 219, no. Pt 10, 2016, pp. 1476-87.
Banerjee S, Tsutsui K, Chaturvedi CM. Apoptosis-mediated testicular alteration in Japanese quail (Coturnix coturnix japonica) in response to temporal phase relation of serotonergic and dopaminergic oscillations. J Exp Biol. 2016;219(Pt 10):1476-87.
Banerjee, S., Tsutsui, K., & Chaturvedi, C. M. (2016). Apoptosis-mediated testicular alteration in Japanese quail (Coturnix coturnix japonica) in response to temporal phase relation of serotonergic and dopaminergic oscillations. The Journal of Experimental Biology, 219(Pt 10), 1476-87. https://doi.org/10.1242/jeb.129155
Banerjee S, Tsutsui K, Chaturvedi CM. Apoptosis-mediated Testicular Alteration in Japanese Quail (Coturnix Coturnix Japonica) in Response to Temporal Phase Relation of Serotonergic and Dopaminergic Oscillations. J Exp Biol. 2016 05 15;219(Pt 10):1476-87. PubMed PMID: 27207953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis-mediated testicular alteration in Japanese quail (Coturnix coturnix japonica) in response to temporal phase relation of serotonergic and dopaminergic oscillations. AU - Banerjee,Somanshu, AU - Tsutsui,Kazuyoshi, AU - Chaturvedi,Chandra Mohini, PY - 2015/07/30/received PY - 2016/02/03/accepted PY - 2016/5/22/entrez PY - 2016/5/22/pubmed PY - 2017/8/5/medline KW - 5-HTP KW - Apoptosis KW - Japanese quail KW - Neural oscillations KW - Testis KW - l-DOPA SP - 1476 EP - 87 JF - The Journal of experimental biology JO - J. Exp. Biol. VL - 219 IS - Pt 10 N2 - Reproductive performance of many avian species, including Japanese quail, is reported to be modulated by specific temporal phase relation of serotonergic and dopaminergic oscillations. Accordingly, it has been shown that the serotonin precursor 5-HTP and the dopamine precursor l-DOPA given 8 h apart induce gonadal suppression and given 12 h apart lead to gonadal stimulation, while other temporal relationships were found to be ineffective. In the present study, we investigated the effects of 8- and 12-h phase relation of neural oscillations on testicular responses including expression of GnRH-I, GnIH, pro-apoptotic proteins (p53 and Bax), inactive and active executioner caspase-3, and the uncleaved DNA repair enzyme PARP-1. Testicular volume and mass decreased significantly in 8-h quail and increased in 12-h quail compared with controls. Expression of ir-GnIH, p53, Bax and active-caspase-3 increased and that of GnRH-I, pro-caspase-3 and uncleaved PARP-1 decreased in 8-h quail compared with controls. A TUNEL assay also confirmed testicular regression in these quail. Testes of 12-h quail exhibited significantly increased expression of GnRH-I, pro-caspase-3 and uncleaved PARP-1 compared with the control group. Our findings suggest that differential response of avian testes to 8- and 12-h phase relation of serotonergic and dopaminergic neural oscillations may be attributed to autocrine/paracrine action of GnIH expression, which is upregulated in regressed testes, leading to apoptotic changes, and downregulated in developed testes, causing apoptotic inhibition. It is concluded that specific phase relation of neural oscillations may modulate the local testicular GnRH-GnIH system and alter the apoptotic mechanism in quail testes. Moreover, these findings highlight the physiological effects of time-dependent drug delivery, including the specific time intervals between two drugs. SN - 1477-9145 UR - https://www.unboundmedicine.com/medline/citation/27207953/Apoptosis_mediated_testicular_alteration_in_Japanese_quail__Coturnix_coturnix_japonica__in_response_to_temporal_phase_relation_of_serotonergic_and_dopaminergic_oscillations_ L2 - http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=27207953 DB - PRIME DP - Unbound Medicine ER -