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Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images?
Plant Physiol. 2016 06; 171(2):1230-41.PP

Abstract

The biosynthesis of the main auxin in plants (indole-3-acetic acid [IAA]) has been elucidated recently and is thought to involve the sequential conversion of Trp to indole-3-pyruvic acid to IAA However, the pathway leading to a less well studied auxin, phenylacetic acid (PAA), remains unclear. Here, we present evidence from metabolism experiments that PAA is synthesized from the amino acid Phe, via phenylpyruvate. In pea (Pisum sativum), the reverse reaction, phenylpyruvate to Phe, is also demonstrated. However, despite similarities between the pathways leading to IAA and PAA, evidence from mutants in pea and maize (Zea mays) indicate that IAA biosynthetic enzymes are not the main enzymes for PAA biosynthesis. Instead, we identified a putative aromatic aminotransferase (PsArAT) from pea that may function in the PAA synthesis pathway.

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Authors+Show Affiliations

Cook SD
School of Biological Sciences (S.D.C., E.L.M., L.Q., J.J.R.), Central Science Laboratory (D.S.N.), School of Chemistry (J.S.), University of Tasmania, Sandy Bay, Tasmania, Australia, 7005; and U.S. Department of Agriculture, Agricultural Research Service, Gainesville, Florida 32608 (P.S.C.).
Nichols DS
School of Biological Sciences (S.D.C., E.L.M., L.Q., J.J.R.), Central Science Laboratory (D.S.N.), School of Chemistry (J.S.), University of Tasmania, Sandy Bay, Tasmania, Australia, 7005; and U.S. Department of Agriculture, Agricultural Research Service, Gainesville, Florida 32608 (P.S.C.).
Smith J
School of Biological Sciences (S.D.C., E.L.M., L.Q., J.J.R.), Central Science Laboratory (D.S.N.), School of Chemistry (J.S.), University of Tasmania, Sandy Bay, Tasmania, Australia, 7005; and U.S. Department of Agriculture, Agricultural Research Service, Gainesville, Florida 32608 (P.S.C.).
Chourey PS
School of Biological Sciences (S.D.C., E.L.M., L.Q., J.J.R.), Central Science Laboratory (D.S.N.), School of Chemistry (J.S.), University of Tasmania, Sandy Bay, Tasmania, Australia, 7005; and U.S. Department of Agriculture, Agricultural Research Service, Gainesville, Florida 32608 (P.S.C.).
McAdam EL
School of Biological Sciences (S.D.C., E.L.M., L.Q., J.J.R.), Central Science Laboratory (D.S.N.), School of Chemistry (J.S.), University of Tasmania, Sandy Bay, Tasmania, Australia, 7005; and U.S. Department of Agriculture, Agricultural Research Service, Gainesville, Florida 32608 (P.S.C.).
Quittenden L
School of Biological Sciences (S.D.C., E.L.M., L.Q., J.J.R.), Central Science Laboratory (D.S.N.), School of Chemistry (J.S.), University of Tasmania, Sandy Bay, Tasmania, Australia, 7005; and U.S. Department of Agriculture, Agricultural Research Service, Gainesville, Florida 32608 (P.S.C.).
Ross JJ
School of Biological Sciences (S.D.C., E.L.M., L.Q., J.J.R.), Central Science Laboratory (D.S.N.), School of Chemistry (J.S.), University of Tasmania, Sandy Bay, Tasmania, Australia, 7005; and U.S. Department of Agriculture, Agricultural Research Service, Gainesville, Florida 32608 (P.S.C.) john.ross@utas.edu.au.

MeSH

Biosynthetic PathwaysChromatography, High Pressure LiquidEnzyme AssaysGenes, PlantIndoleacetic AcidsIndolesMass SpectrometryMutationPeasPhenylacetatesPhenylalaninePlant ProteinsTryptophanZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27208245

Citation

Cook, Sam D., et al. "Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images?" Plant Physiology, vol. 171, no. 2, 2016, pp. 1230-41.
Cook SD, Nichols DS, Smith J, et al. Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images? Plant Physiol. 2016;171(2):1230-41.
Cook, S. D., Nichols, D. S., Smith, J., Chourey, P. S., McAdam, E. L., Quittenden, L., & Ross, J. J. (2016). Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images? Plant Physiology, 171(2), 1230-41. https://doi.org/10.1104/pp.16.00454
Cook SD, et al. Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images. Plant Physiol. 2016;171(2):1230-41. PubMed PMID: 27208245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images? AU - Cook,Sam D, AU - Nichols,David S, AU - Smith,Jason, AU - Chourey,Prem S, AU - McAdam,Erin L, AU - Quittenden,Laura, AU - Ross,John J, Y1 - 2016/04/26/ PY - 2016/04/05/received PY - 2016/04/21/accepted PY - 2016/5/22/entrez PY - 2016/5/22/pubmed PY - 2017/9/20/medline SP - 1230 EP - 41 JF - Plant physiology JO - Plant Physiol VL - 171 IS - 2 N2 - The biosynthesis of the main auxin in plants (indole-3-acetic acid [IAA]) has been elucidated recently and is thought to involve the sequential conversion of Trp to indole-3-pyruvic acid to IAA However, the pathway leading to a less well studied auxin, phenylacetic acid (PAA), remains unclear. Here, we present evidence from metabolism experiments that PAA is synthesized from the amino acid Phe, via phenylpyruvate. In pea (Pisum sativum), the reverse reaction, phenylpyruvate to Phe, is also demonstrated. However, despite similarities between the pathways leading to IAA and PAA, evidence from mutants in pea and maize (Zea mays) indicate that IAA biosynthetic enzymes are not the main enzymes for PAA biosynthesis. Instead, we identified a putative aromatic aminotransferase (PsArAT) from pea that may function in the PAA synthesis pathway. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/27208245/Auxin_Biosynthesis:_Are_the_Indole_3_Acetic_Acid_and_Phenylacetic_Acid_Biosynthesis_Pathways_Mirror_Images DB - PRIME DP - Unbound Medicine ER -