Tags

Type your tag names separated by a space and hit enter

Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense.
Plant Physiol 2016; 171(2):1427-42PP

Abstract

The WRKY family of transcription factors (TFs) functions as transcriptional activators or repressors in various signaling pathways. In this study, we discovered that OsWRKY62 and OsWRKY76, two genes of the WRKY IIa subfamily, undergo constitutive and inducible alternative splicing. The full-length OsWRKY62.1 and OsWRKY76.1 proteins formed homocomplexes and heterocomplexes, and the heterocomplex dominates in the nuclei when analyzed in Nicotiana benthamiana leaves. Transgenic overexpression of OsWRKY62.1 and OsWRKY76.1 in rice (Oryza sativa) enhanced plant susceptibility to the blast fungus Magnaporthe oryzae and the leaf blight bacterium Xanthomonas oryzae pv oryzae, whereas RNA interference and loss-of-function knockout plants exhibited elevated resistance. The dsOW62/76 and knockout lines of OsWRKY62 and OsWRKY76 also showed greatly increased expression of defense-related genes and the accumulation of phytoalexins. The ratio of full-length versus truncated transcripts changed in dsOW62/76 plants as well as in response to pathogen infection. The short alternative OsWRKY62.2 and OsWRKY76.2 isoforms could interact with each other and with full-length proteins. OsWRKY62.2 showed a reduced repressor activity in planta, and two sequence determinants required for the repressor activity were identified in the amino terminus of OsWRKY62.1. The amino termini of OsWRKY62 and OsWRKY76 splice variants also showed reduced binding to the canonical W box motif. These results not only enhance our understanding of the DNA-binding property, the repressor sequence motifs, and the negative feedback regulation of the IIa subfamily of WRKYs but also provide evidence for alternative splicing of WRKY TFs during the plant defense response.

Authors+Show Affiliations

Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.).Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.).Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.).Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.).Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.).Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.).Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.).Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.) guozj@cau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27208272

Citation

Liu, Jiqin, et al. "Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense." Plant Physiology, vol. 171, no. 2, 2016, pp. 1427-42.
Liu J, Chen X, Liang X, et al. Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense. Plant Physiol. 2016;171(2):1427-42.
Liu, J., Chen, X., Liang, X., Zhou, X., Yang, F., Liu, J., ... Guo, Z. (2016). Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense. Plant Physiology, 171(2), pp. 1427-42. doi:10.1104/pp.15.01921.
Liu J, et al. Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense. Plant Physiol. 2016;171(2):1427-42. PubMed PMID: 27208272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense. AU - Liu,Jiqin, AU - Chen,Xujun, AU - Liang,Xiaoxing, AU - Zhou,Xiangui, AU - Yang,Fang, AU - Liu,Jia, AU - He,Sheng Yang, AU - Guo,Zejian, Y1 - 2016/04/18/ PY - 2015/12/08/received PY - 2016/04/13/accepted PY - 2016/5/22/entrez PY - 2016/5/22/pubmed PY - 2017/9/20/medline SP - 1427 EP - 42 JF - Plant physiology JO - Plant Physiol. VL - 171 IS - 2 N2 - The WRKY family of transcription factors (TFs) functions as transcriptional activators or repressors in various signaling pathways. In this study, we discovered that OsWRKY62 and OsWRKY76, two genes of the WRKY IIa subfamily, undergo constitutive and inducible alternative splicing. The full-length OsWRKY62.1 and OsWRKY76.1 proteins formed homocomplexes and heterocomplexes, and the heterocomplex dominates in the nuclei when analyzed in Nicotiana benthamiana leaves. Transgenic overexpression of OsWRKY62.1 and OsWRKY76.1 in rice (Oryza sativa) enhanced plant susceptibility to the blast fungus Magnaporthe oryzae and the leaf blight bacterium Xanthomonas oryzae pv oryzae, whereas RNA interference and loss-of-function knockout plants exhibited elevated resistance. The dsOW62/76 and knockout lines of OsWRKY62 and OsWRKY76 also showed greatly increased expression of defense-related genes and the accumulation of phytoalexins. The ratio of full-length versus truncated transcripts changed in dsOW62/76 plants as well as in response to pathogen infection. The short alternative OsWRKY62.2 and OsWRKY76.2 isoforms could interact with each other and with full-length proteins. OsWRKY62.2 showed a reduced repressor activity in planta, and two sequence determinants required for the repressor activity were identified in the amino terminus of OsWRKY62.1. The amino termini of OsWRKY62 and OsWRKY76 splice variants also showed reduced binding to the canonical W box motif. These results not only enhance our understanding of the DNA-binding property, the repressor sequence motifs, and the negative feedback regulation of the IIa subfamily of WRKYs but also provide evidence for alternative splicing of WRKY TFs during the plant defense response. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/27208272/Alternative_Splicing_of_Rice_WRKY62_and_WRKY76_Transcription_Factor_Genes_in_Pathogen_Defense_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=27208272 DB - PRIME DP - Unbound Medicine ER -