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The WD-Repeat Protein CsTTG1 Regulates Fruit Wart Formation through Interaction with the Homeodomain-Leucine Zipper I Protein Mict.
Plant Physiol. 2016 06; 171(2):1156-68.PP

Abstract

The cucumber (Cucumis sativus) fruit is covered with bloom trichomes and warts (composed of spines and tubercules), which have an important impact on the commercial value of the crop. However, little is known about the regulatory mechanism underlying their formation. Here, we reported that the cucumber WD-repeat homolog CsTTG1, which is localized in the nucleus and cytomembrane, plays an important role in the formation of cucumber fruit bloom trichomes and warts. Functional characterization of CsTTG1 revealed that it is mainly expressed in the epidermis of cucumber ovary and that its overexpression in cucumber alters the density of fruit bloom trichomes and spines, thereby promoting the warty fruit trait. Conversely, silencing CsTTG1 expression inhibits the initiation of fruit spines. Molecular and genetic analyses showed that CsTTG1 acts in parallel to Mict/CsGL1, a key trichome formation factor, to regulate the initiation of fruit trichomes, including fruit bloom trichomes and spines, and that the further differentiation of fruit spines and formation of tubercules regulated by CsTTG1 is dependent on Mict Using yeast two-hybrid assay and bimolecular fluorescence complementation assay, we determined that CsTTG1 directly interacts with Mict. Collectively, our results indicate that CsTTG1 is an important component of the molecular network that regulates fruit bloom trichome and wart formation in cucumber.

Authors+Show Affiliations

Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, P.R. China renhuazhong@cau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27208299

Citation

Chen, Chunhua, et al. "The WD-Repeat Protein CsTTG1 Regulates Fruit Wart Formation Through Interaction With the Homeodomain-Leucine Zipper I Protein Mict." Plant Physiology, vol. 171, no. 2, 2016, pp. 1156-68.
Chen C, Yin S, Liu X, et al. The WD-Repeat Protein CsTTG1 Regulates Fruit Wart Formation through Interaction with the Homeodomain-Leucine Zipper I Protein Mict. Plant Physiol. 2016;171(2):1156-68.
Chen, C., Yin, S., Liu, X., Liu, B., Yang, S., Xue, S., Cai, Y., Black, K., Liu, H., Dong, M., Zhang, Y., Zhao, B., & Ren, H. (2016). The WD-Repeat Protein CsTTG1 Regulates Fruit Wart Formation through Interaction with the Homeodomain-Leucine Zipper I Protein Mict. Plant Physiology, 171(2), 1156-68. https://doi.org/10.1104/pp.16.00112
Chen C, et al. The WD-Repeat Protein CsTTG1 Regulates Fruit Wart Formation Through Interaction With the Homeodomain-Leucine Zipper I Protein Mict. Plant Physiol. 2016;171(2):1156-68. PubMed PMID: 27208299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The WD-Repeat Protein CsTTG1 Regulates Fruit Wart Formation through Interaction with the Homeodomain-Leucine Zipper I Protein Mict. AU - Chen,Chunhua, AU - Yin,Shuai, AU - Liu,Xingwang, AU - Liu,Bin, AU - Yang,Sen, AU - Xue,Shudan, AU - Cai,Yanling, AU - Black,Kezia, AU - Liu,Huiling, AU - Dong,Mingming, AU - Zhang,Yaqi, AU - Zhao,Binyu, AU - Ren,Huazhong, Y1 - 2016/04/20/ PY - 2016/02/22/received PY - 2016/04/18/accepted PY - 2016/5/22/entrez PY - 2016/5/22/pubmed PY - 2017/9/20/medline SP - 1156 EP - 68 JF - Plant physiology JO - Plant Physiol VL - 171 IS - 2 N2 - The cucumber (Cucumis sativus) fruit is covered with bloom trichomes and warts (composed of spines and tubercules), which have an important impact on the commercial value of the crop. However, little is known about the regulatory mechanism underlying their formation. Here, we reported that the cucumber WD-repeat homolog CsTTG1, which is localized in the nucleus and cytomembrane, plays an important role in the formation of cucumber fruit bloom trichomes and warts. Functional characterization of CsTTG1 revealed that it is mainly expressed in the epidermis of cucumber ovary and that its overexpression in cucumber alters the density of fruit bloom trichomes and spines, thereby promoting the warty fruit trait. Conversely, silencing CsTTG1 expression inhibits the initiation of fruit spines. Molecular and genetic analyses showed that CsTTG1 acts in parallel to Mict/CsGL1, a key trichome formation factor, to regulate the initiation of fruit trichomes, including fruit bloom trichomes and spines, and that the further differentiation of fruit spines and formation of tubercules regulated by CsTTG1 is dependent on Mict Using yeast two-hybrid assay and bimolecular fluorescence complementation assay, we determined that CsTTG1 directly interacts with Mict. Collectively, our results indicate that CsTTG1 is an important component of the molecular network that regulates fruit bloom trichome and wart formation in cucumber. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/27208299/The_WD_Repeat_Protein_CsTTG1_Regulates_Fruit_Wart_Formation_through_Interaction_with_the_Homeodomain_Leucine_Zipper_I_Protein_Mict_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=27208299 DB - PRIME DP - Unbound Medicine ER -