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Assembling Composite Dermal Papilla Spheres with Adipose-derived Stem Cells to Enhance Hair Follicle Induction.
Sci Rep. 2016 05 23; 6:26436.SR

Abstract

Intradermal adipose tissue plays an essential role for hair follicles (HFs) regeneration by regulating hair cycles. However, the effect of reconstruction of HFs and the involvement of adipose-related cells are poorly understood. We investigated assembly strategies for the interactions of dermal papilla (DP) cells with adipose-derived stem cells (ASCs) in promoting hair formation. DP cells lose DP traits during adherent culture, but preserved DP markers with a unified sphere diameter by seeding on chitosan-coated microenvironments. Next, ASCs isolated from rats were co-cultured with DP spheres by different assembling approaches to determine their interactions; a mixed sphere of ASCs with DP cells (MA-DPS), or a core-shell structure, outer ASCs shell and an inner DP core (CSA-DPS). CSA-DPS exhibited superior DP characteristics compared to MA-DPS. Conditional medium from ASCs, but not differentiated adipocytes, promoted DP markers and functional alkaline phosphatase activity from the DP cells. In vivo patch assay showed the core-shell assembling of CSA-DPS can reconstruct cellular arrangements and microenvironmental niches as dominated by PPARα signal in ASCs to induce the greater hair induction than MA-DPS or DP spheres alone. Therefore, the assembling of a core-shell sphere for DP with ASCs could reconstruct the HF cellular arrangement for hair formation. This paper set the groundwork for further evaluation of the input of other cell types.

Authors+Show Affiliations

Department of Biomedical Engineering, National Cheng Kung University, Tainan, 701, Taiwan.Institute of Basic Medical Science, National Cheng Kung University, Tainan, 701, Taiwan.Division of Plastic Surgery, National Cheng Kung University Hospital, Tainan, 701, Taiwan.Institute of Basic Medical Science, National Cheng Kung University, Tainan, 701, Taiwan.Division of Plastic Surgery, National Cheng Kung University Hospital, Tainan, 701, Taiwan.Institute of Basic Medical Science, National Cheng Kung University, Tainan, 701, Taiwan.Division of Plastic Surgery, National Cheng Kung University Hospital, Tainan, 701, Taiwan. Department of Cell Biology and Anatomy, National Cheng Kung University, Tainan, 701, Taiwan.Department of Biomedical Engineering, National Cheng Kung University, Tainan, 701, Taiwan.International Research Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, 701, Taiwan. Institute of Clinical Medicine, National Cheng Kung University, Tainan, 701, Taiwan.International Research Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, 701, Taiwan. Institute of Clinical Medicine, National Cheng Kung University, Tainan, 701, Taiwan. Department of Pathology, University of Southern California, California 90033, USA.Department of Biomedical Engineering, National Cheng Kung University, Tainan, 701, Taiwan. Institute of Basic Medical Science, National Cheng Kung University, Tainan, 701, Taiwan. Department of Cell Biology and Anatomy, National Cheng Kung University, Tainan, 701, Taiwan. International Research Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, 701, Taiwan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27210831

Citation

Huang, Chin-Fu, et al. "Assembling Composite Dermal Papilla Spheres With Adipose-derived Stem Cells to Enhance Hair Follicle Induction." Scientific Reports, vol. 6, 2016, p. 26436.
Huang CF, Chang YJ, Hsueh YY, et al. Assembling Composite Dermal Papilla Spheres with Adipose-derived Stem Cells to Enhance Hair Follicle Induction. Sci Rep. 2016;6:26436.
Huang, C. F., Chang, Y. J., Hsueh, Y. Y., Huang, C. W., Wang, D. H., Huang, T. C., Wu, Y. T., Su, F. C., Hughes, M., Chuong, C. M., & Wu, C. C. (2016). Assembling Composite Dermal Papilla Spheres with Adipose-derived Stem Cells to Enhance Hair Follicle Induction. Scientific Reports, 6, 26436. https://doi.org/10.1038/srep26436
Huang CF, et al. Assembling Composite Dermal Papilla Spheres With Adipose-derived Stem Cells to Enhance Hair Follicle Induction. Sci Rep. 2016 05 23;6:26436. PubMed PMID: 27210831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assembling Composite Dermal Papilla Spheres with Adipose-derived Stem Cells to Enhance Hair Follicle Induction. AU - Huang,Chin-Fu, AU - Chang,Ya-Ju, AU - Hsueh,Yuan-Yu, AU - Huang,Chia-Wei, AU - Wang,Duo-Hsiang, AU - Huang,Tzu-Chieh, AU - Wu,Yi-Ting, AU - Su,Fong-Chin, AU - Hughes,Michael, AU - Chuong,Cheng-Ming, AU - Wu,Chia-Ching, Y1 - 2016/05/23/ PY - 2016/02/19/received PY - 2016/05/03/accepted PY - 2016/5/24/entrez PY - 2016/5/24/pubmed PY - 2018/4/24/medline SP - 26436 EP - 26436 JF - Scientific reports JO - Sci Rep VL - 6 N2 - Intradermal adipose tissue plays an essential role for hair follicles (HFs) regeneration by regulating hair cycles. However, the effect of reconstruction of HFs and the involvement of adipose-related cells are poorly understood. We investigated assembly strategies for the interactions of dermal papilla (DP) cells with adipose-derived stem cells (ASCs) in promoting hair formation. DP cells lose DP traits during adherent culture, but preserved DP markers with a unified sphere diameter by seeding on chitosan-coated microenvironments. Next, ASCs isolated from rats were co-cultured with DP spheres by different assembling approaches to determine their interactions; a mixed sphere of ASCs with DP cells (MA-DPS), or a core-shell structure, outer ASCs shell and an inner DP core (CSA-DPS). CSA-DPS exhibited superior DP characteristics compared to MA-DPS. Conditional medium from ASCs, but not differentiated adipocytes, promoted DP markers and functional alkaline phosphatase activity from the DP cells. In vivo patch assay showed the core-shell assembling of CSA-DPS can reconstruct cellular arrangements and microenvironmental niches as dominated by PPARα signal in ASCs to induce the greater hair induction than MA-DPS or DP spheres alone. Therefore, the assembling of a core-shell sphere for DP with ASCs could reconstruct the HF cellular arrangement for hair formation. This paper set the groundwork for further evaluation of the input of other cell types. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27210831/Assembling_Composite_Dermal_Papilla_Spheres_with_Adipose_derived_Stem_Cells_to_Enhance_Hair_Follicle_Induction_ L2 - https://doi.org/10.1038/srep26436 DB - PRIME DP - Unbound Medicine ER -