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The Fd-GOGAT1 mutant gene lc7 confers resistance to Xanthomonas oryzae pv. Oryzae in rice.
Sci Rep. 2016 05 23; 6:26411.SR

Abstract

Disease resistance is an important goal of crop improvement. The molecular mechanism of resistance requires further study. Here, we report the identification of a rice leaf color mutant, lc7, which is defective in chlorophyll synthesis and photosynthesis but confers resistance to Xanthomonas oryzae pv. Oryzae (Xoo). Map-based cloning revealed that lc7 encodes a mutant ferredoxin-dependent glutamate synthase1 (Fd-GOGAT1). Fd-GOGAT1 has been proposed to have great potential for improving nitrogen-use efficiency, but its function in bacterial resistance has not been reported. The lc7 mutant accumulates excessive levels of ROS (reactive oxygen species) in the leaves, causing the leaf color to become yellow after the four-leaf stage. Compared to the wild type, lc7 mutants have a broad-spectrum high resistance to seven Xoo strains. Differentially expressed genes (DEGs) and qRT-PCR analysis indicate that many defense pathways that are involved in this broad-spectrum resistance are activated in the lc7 mutant. These results suggest that Fd-GOGAT1 plays an important role in broad-spectrum bacterial blight resistance, in addition to modulating nitrogen assimilation and chloroplast development.

Authors+Show Affiliations

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27211925

Citation

Chen, Honglin, et al. "The Fd-GOGAT1 Mutant Gene Lc7 Confers Resistance to Xanthomonas Oryzae Pv. Oryzae in Rice." Scientific Reports, vol. 6, 2016, p. 26411.
Chen H, Li C, Liu L, et al. The Fd-GOGAT1 mutant gene lc7 confers resistance to Xanthomonas oryzae pv. Oryzae in rice. Sci Rep. 2016;6:26411.
Chen, H., Li, C., Liu, L., Zhao, J., Cheng, X., Jiang, G., & Zhai, W. (2016). The Fd-GOGAT1 mutant gene lc7 confers resistance to Xanthomonas oryzae pv. Oryzae in rice. Scientific Reports, 6, 26411. https://doi.org/10.1038/srep26411
Chen H, et al. The Fd-GOGAT1 Mutant Gene Lc7 Confers Resistance to Xanthomonas Oryzae Pv. Oryzae in Rice. Sci Rep. 2016 05 23;6:26411. PubMed PMID: 27211925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Fd-GOGAT1 mutant gene lc7 confers resistance to Xanthomonas oryzae pv. Oryzae in rice. AU - Chen,Honglin, AU - Li,Chunrong, AU - Liu,Liping, AU - Zhao,Jiying, AU - Cheng,Xuzhen, AU - Jiang,Guanghuai, AU - Zhai,Wenxue, Y1 - 2016/05/23/ PY - 2015/12/04/received PY - 2016/05/03/accepted PY - 2016/5/24/entrez PY - 2016/5/24/pubmed PY - 2018/4/24/medline SP - 26411 EP - 26411 JF - Scientific reports JO - Sci Rep VL - 6 N2 - Disease resistance is an important goal of crop improvement. The molecular mechanism of resistance requires further study. Here, we report the identification of a rice leaf color mutant, lc7, which is defective in chlorophyll synthesis and photosynthesis but confers resistance to Xanthomonas oryzae pv. Oryzae (Xoo). Map-based cloning revealed that lc7 encodes a mutant ferredoxin-dependent glutamate synthase1 (Fd-GOGAT1). Fd-GOGAT1 has been proposed to have great potential for improving nitrogen-use efficiency, but its function in bacterial resistance has not been reported. The lc7 mutant accumulates excessive levels of ROS (reactive oxygen species) in the leaves, causing the leaf color to become yellow after the four-leaf stage. Compared to the wild type, lc7 mutants have a broad-spectrum high resistance to seven Xoo strains. Differentially expressed genes (DEGs) and qRT-PCR analysis indicate that many defense pathways that are involved in this broad-spectrum resistance are activated in the lc7 mutant. These results suggest that Fd-GOGAT1 plays an important role in broad-spectrum bacterial blight resistance, in addition to modulating nitrogen assimilation and chloroplast development. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27211925/The_Fd_GOGAT1_mutant_gene_lc7_confers_resistance_to_Xanthomonas_oryzae_pv__Oryzae_in_rice_ L2 - https://doi.org/10.1038/srep26411 DB - PRIME DP - Unbound Medicine ER -