Tags

Type your tag names separated by a space and hit enter

Fluoroquinolone-Resistant Sequence Type 131 Subgroups O25b and O16 Among Extraintestinal Escherichia coli Isolates from Community-Acquired Urinary Tract Infections.
Microb Drug Resist. 2017 Mar; 23(2):224-229.MD

Abstract

The multidrug-resistant sequence type 131 (ST131) Escherichia coli is a spreading epidemiological burden particularly among isolates resistant to fluoroquinolones. We aimed to evaluate the commonality of ST131-O25b and ST131-O16 among fluoroquinolone-resistant E. coli isolates causing community-acquired urinary tract infections (UTIs) at Fayoum University Hospital, in Egypt. Ninety-two fluoroquinolone-resistant E. coli isolates were subjected to multiplex PCR for detection of ST131 of either O25b or O16 subgroups. Positive isolates were then assessed for antimicrobial susceptibility and virulence genotyping. Out of 92 fluoroquinolone-resistant E. coli isolates, 56 (60.9%) isolates were O25b/O16 subgroups of ST131, including 44 (78.6%) ST131-O25b and 12 (21.4%) ST131-O16 subgroups. All the O25b/O16 ST131 isolates were sensitive to meropenem, where ST131-O25b isolates were significantly more resistant to extended spectrum cephalosporins compared to S131-O16 strains. All the O25b/O16 ST131 isolates harbored three or more of the virulence factors associated with extraintestinal pathogenic E. coli status. ST131-O16 showed a significantly higher virulence score than ST131-O25b isolates. Our results bring to highlight the emergence of O25b/O16 ST131 isolates between community acquired UTIs among Egyptian patients. This is the first report for the presence of O16 isolates in Egypt, showing a lower predominance than the O25b subgroup. The high prevalence of O25b/O16 ST131 isolates requires strict stewardship on antimicrobial use, notably fluoroquinolones, to control the endemicity of such emerging multidrug-resistant clone in the community.

Authors+Show Affiliations

1 Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University , Fayoum, Egypt .2 Department of Microbiology and Immunology, Faculty of Medicine, Minia University , Minia, Egypt .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27214282

Citation

Hefzy, Enas Mamdouh, and Noha Anwar Hassuna. "Fluoroquinolone-Resistant Sequence Type 131 Subgroups O25b and O16 Among Extraintestinal Escherichia Coli Isolates From Community-Acquired Urinary Tract Infections." Microbial Drug Resistance (Larchmont, N.Y.), vol. 23, no. 2, 2017, pp. 224-229.
Hefzy EM, Hassuna NA. Fluoroquinolone-Resistant Sequence Type 131 Subgroups O25b and O16 Among Extraintestinal Escherichia coli Isolates from Community-Acquired Urinary Tract Infections. Microb Drug Resist. 2017;23(2):224-229.
Hefzy, E. M., & Hassuna, N. A. (2017). Fluoroquinolone-Resistant Sequence Type 131 Subgroups O25b and O16 Among Extraintestinal Escherichia coli Isolates from Community-Acquired Urinary Tract Infections. Microbial Drug Resistance (Larchmont, N.Y.), 23(2), 224-229. https://doi.org/10.1089/mdr.2016.0040
Hefzy EM, Hassuna NA. Fluoroquinolone-Resistant Sequence Type 131 Subgroups O25b and O16 Among Extraintestinal Escherichia Coli Isolates From Community-Acquired Urinary Tract Infections. Microb Drug Resist. 2017;23(2):224-229. PubMed PMID: 27214282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluoroquinolone-Resistant Sequence Type 131 Subgroups O25b and O16 Among Extraintestinal Escherichia coli Isolates from Community-Acquired Urinary Tract Infections. AU - Hefzy,Enas Mamdouh, AU - Hassuna,Noha Anwar, Y1 - 2016/05/23/ PY - 2016/5/24/pubmed PY - 2017/6/27/medline PY - 2016/5/24/entrez KW - E. coli KW - ST131 KW - UTI SP - 224 EP - 229 JF - Microbial drug resistance (Larchmont, N.Y.) JO - Microb Drug Resist VL - 23 IS - 2 N2 - The multidrug-resistant sequence type 131 (ST131) Escherichia coli is a spreading epidemiological burden particularly among isolates resistant to fluoroquinolones. We aimed to evaluate the commonality of ST131-O25b and ST131-O16 among fluoroquinolone-resistant E. coli isolates causing community-acquired urinary tract infections (UTIs) at Fayoum University Hospital, in Egypt. Ninety-two fluoroquinolone-resistant E. coli isolates were subjected to multiplex PCR for detection of ST131 of either O25b or O16 subgroups. Positive isolates were then assessed for antimicrobial susceptibility and virulence genotyping. Out of 92 fluoroquinolone-resistant E. coli isolates, 56 (60.9%) isolates were O25b/O16 subgroups of ST131, including 44 (78.6%) ST131-O25b and 12 (21.4%) ST131-O16 subgroups. All the O25b/O16 ST131 isolates were sensitive to meropenem, where ST131-O25b isolates were significantly more resistant to extended spectrum cephalosporins compared to S131-O16 strains. All the O25b/O16 ST131 isolates harbored three or more of the virulence factors associated with extraintestinal pathogenic E. coli status. ST131-O16 showed a significantly higher virulence score than ST131-O25b isolates. Our results bring to highlight the emergence of O25b/O16 ST131 isolates between community acquired UTIs among Egyptian patients. This is the first report for the presence of O16 isolates in Egypt, showing a lower predominance than the O25b subgroup. The high prevalence of O25b/O16 ST131 isolates requires strict stewardship on antimicrobial use, notably fluoroquinolones, to control the endemicity of such emerging multidrug-resistant clone in the community. SN - 1931-8448 UR - https://www.unboundmedicine.com/medline/citation/27214282/Fluoroquinolone_Resistant_Sequence_Type_131_Subgroups_O25b_and_O16_Among_Extraintestinal_Escherichia_coli_Isolates_from_Community_Acquired_Urinary_Tract_Infections_ L2 - https://www.liebertpub.com/doi/10.1089/mdr.2016.0040?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -