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Bombyx mori E26 transformation-specific 2 (BmEts2), an Ets family protein, represses Bombyx mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in the silkworm Bombyx mori.
Insect Mol Biol. 2016 10; 25(5):566-79.IM

Abstract

E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2, which is a putative orthologue of Drosophila Yan and human translocation-ets-leukemia/Ets-variant gene 6, from the silkworm Bombyx mori. Expression of the BmEts2 gene was significantly increased in the fat bodies of silkworm larvae in response to injection with Escherichia coli and Staphylococcus aureus. BmEts2 overexpression dramatically repressed B. mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in silkworm cells. Conversely, gene knockdown of BmEts2 significantly enhanced BmRels activity. In addition, two κB sites located on the 5' upstream region of cecropin B1 were found to be involved in the repression of BmRels-mediated promoter activation. Protein-competition analysis further demonstrated that BmEts2 competitively inhibited binding of BmRels to κB sites. Overall, BmEts2 acts as a repressor of BmRels-mediated transactivation of antimicrobial protein genes by inhibiting the binding of BmRels to κB sites.

Authors+Show Affiliations

Insect-Microbe Research Unit, National Institute of Agrobiological Sciences, Ibaraki, Japan.Insect-Microbe Research Unit, National Institute of Agrobiological Sciences, Ibaraki, Japan.Division of Insect Sciences, National Institute of Agrobiological Sciences, Ibaraki, Japan.Division of Insect Sciences, National Institute of Agrobiological Sciences, Ibaraki, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27227900

Citation

Tanaka, H, et al. "Bombyx Mori E26 Transformation-specific 2 (BmEts2), an Ets Family Protein, Represses Bombyx Mori Rels (BmRels)-mediated Promoter Activation of Antimicrobial Peptide Genes in the Silkworm Bombyx Mori." Insect Molecular Biology, vol. 25, no. 5, 2016, pp. 566-79.
Tanaka H, Sagisaka A, Suzuki N, et al. Bombyx mori E26 transformation-specific 2 (BmEts2), an Ets family protein, represses Bombyx mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in the silkworm Bombyx mori. Insect Mol Biol. 2016;25(5):566-79.
Tanaka, H., Sagisaka, A., Suzuki, N., & Yamakawa, M. (2016). Bombyx mori E26 transformation-specific 2 (BmEts2), an Ets family protein, represses Bombyx mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in the silkworm Bombyx mori. Insect Molecular Biology, 25(5), 566-79. https://doi.org/10.1111/imb.12244
Tanaka H, et al. Bombyx Mori E26 Transformation-specific 2 (BmEts2), an Ets Family Protein, Represses Bombyx Mori Rels (BmRels)-mediated Promoter Activation of Antimicrobial Peptide Genes in the Silkworm Bombyx Mori. Insect Mol Biol. 2016;25(5):566-79. PubMed PMID: 27227900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bombyx mori E26 transformation-specific 2 (BmEts2), an Ets family protein, represses Bombyx mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in the silkworm Bombyx mori. AU - Tanaka,H, AU - Sagisaka,A, AU - Suzuki,N, AU - Yamakawa,M, Y1 - 2016/05/26/ PY - 2016/5/27/entrez PY - 2016/5/27/pubmed PY - 2017/9/8/medline KW - Bombyx mori KW - Ets family protein KW - antimicrobial peptides KW - gene regulation KW - silkworm SP - 566 EP - 79 JF - Insect molecular biology JO - Insect Mol. Biol. VL - 25 IS - 5 N2 - E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2, which is a putative orthologue of Drosophila Yan and human translocation-ets-leukemia/Ets-variant gene 6, from the silkworm Bombyx mori. Expression of the BmEts2 gene was significantly increased in the fat bodies of silkworm larvae in response to injection with Escherichia coli and Staphylococcus aureus. BmEts2 overexpression dramatically repressed B. mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in silkworm cells. Conversely, gene knockdown of BmEts2 significantly enhanced BmRels activity. In addition, two κB sites located on the 5' upstream region of cecropin B1 were found to be involved in the repression of BmRels-mediated promoter activation. Protein-competition analysis further demonstrated that BmEts2 competitively inhibited binding of BmRels to κB sites. Overall, BmEts2 acts as a repressor of BmRels-mediated transactivation of antimicrobial protein genes by inhibiting the binding of BmRels to κB sites. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/27227900/Bombyx_mori_E26_transformation_specific_2__BmEts2__an_Ets_family_protein_represses_Bombyx_mori_Rels__BmRels__mediated_promoter_activation_of_antimicrobial_peptide_genes_in_the_silkworm_Bombyx_mori_ L2 - https://doi.org/10.1111/imb.12244 DB - PRIME DP - Unbound Medicine ER -