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Chemical composition and in vitro antibacterial activity of Pistacia terebinthus essential oils derived from wild populations in Kosovo.
BMC Complement Altern Med. 2016 May 26; 16:147.BC

Abstract

BACKGROUND

Plant material from different organs of Pistacia terebinthus L., (Anacardiaceae) were collected in Kosovo with aim to analyze the chemical variability of the essential oils among native populations and to test them for potential antibacterial activity against Staphylococcus aureus.

METHODS

Essential oils obtained from leaves, pedicels, fruits and galls were analyzed by GC-FID and GC/MS. Minimum inhibitory concentration (MIC) against three clinically relevant strains of S. aureus (NRS385, LAC and UAMS-1) were used to evaluate the antibacterial activity of essential oils.

RESULTS

In total, 33 different compounds were identified. The main constituents were α-pinene (12.58-66.29 %), D-limonene (13.95-46.29 %), β-ocimene (0.03-40.49 %), β-pinene (2.63-20.47 %), sabinene (0.00-5.61 %) and (Z)-β-ocimene (0.00-44.85 %). Antibacterial testing of the essential oils against three clinical isolates of S. aureus revealed that seven of the eight samples had some activity at the concentration range tested (0.04-0.512 % v/v). The gall tissues from both sites produced the highest yield of essential oil (3.24 and 6 %), and both exhibited growth inhibitory activity against S. aureus. The most bioactive essential oils, which exhibited MIC90 values ranging from 0.032-0.128 % v/v, obtained from the fruits of the Ura e Shejtë collection site. Likewise, the leaf and pedicel essential oil from the same site was highly active with MIC90 values of 0.064-0.128 and 0.032-0.256 % v/v, respectively.

CONCLUSIONS

Principle Component Analyses demonstrated that there is a variation in the chemical composition of essential oil depending on the plant organs from which essential oil are obtained and the geographical origin of the plant populations. The highest variability regarding the chemical composition of essential oil was found between oils obtained from different organs originating from the Prizren site. The MIC90 activity of Pistacia terebinthus was on par or superior compared with Tea Tree Oil control (0.128 % v/v), suggesting that essential oils from this species may have some potential for development as an antibacterial agent for S. aureus infections.

Authors+Show Affiliations

Department of Biology, Faculty of Mathematical and Natural Science, University of Prishtina "Hasan Prishtina", Mother Theresa St, 10000, Prishtinë, Kosovo.Department of Biology, Faculty of Mathematical and Natural Science, University of Prishtina "Hasan Prishtina", Mother Theresa St, 10000, Prishtinë, Kosovo. Institute for Biological and Environmental Researches, Faculty of Mathematical and Natural Science, University of Prishtina "Hasan Prishtina", Mother Theresa St, 10000, Prishtinë, Kosovo.Department of Dermatology, Emory University School of Medicine, 615 Michael St, Whitehead 105-L, Atlanta, GA, 30322, USA.Department of Dermatology, Emory University School of Medicine, 615 Michael St, Whitehead 105-L, Atlanta, GA, 30322, USA. Center for the Study of Human Health, Emory University, 550 Asbury Circle, Candler Library 107, Atlanta, GA, 30322, USA.Department of Biology, Faculty of Mathematical and Natural Science, University of Prishtina "Hasan Prishtina", Mother Theresa St, 10000, Prishtinë, Kosovo. avni.hajdari@uni-pr.edu. Institute for Biological and Environmental Researches, Faculty of Mathematical and Natural Science, University of Prishtina "Hasan Prishtina", Mother Theresa St, 10000, Prishtinë, Kosovo. avni.hajdari@uni-pr.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27229927

Citation

Pulaj, Bledar, et al. "Chemical Composition and in Vitro Antibacterial Activity of Pistacia Terebinthus Essential Oils Derived From Wild Populations in Kosovo." BMC Complementary and Alternative Medicine, vol. 16, 2016, p. 147.
Pulaj B, Mustafa B, Nelson K, et al. Chemical composition and in vitro antibacterial activity of Pistacia terebinthus essential oils derived from wild populations in Kosovo. BMC Complement Altern Med. 2016;16:147.
Pulaj, B., Mustafa, B., Nelson, K., Quave, C. L., & Hajdari, A. (2016). Chemical composition and in vitro antibacterial activity of Pistacia terebinthus essential oils derived from wild populations in Kosovo. BMC Complementary and Alternative Medicine, 16, 147. https://doi.org/10.1186/s12906-016-1135-8
Pulaj B, et al. Chemical Composition and in Vitro Antibacterial Activity of Pistacia Terebinthus Essential Oils Derived From Wild Populations in Kosovo. BMC Complement Altern Med. 2016 May 26;16:147. PubMed PMID: 27229927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical composition and in vitro antibacterial activity of Pistacia terebinthus essential oils derived from wild populations in Kosovo. AU - Pulaj,Bledar, AU - Mustafa,Behxhet, AU - Nelson,Kate, AU - Quave,Cassandra L, AU - Hajdari,Avni, Y1 - 2016/05/26/ PY - 2015/12/19/received PY - 2016/05/18/accepted PY - 2016/5/28/entrez PY - 2016/5/28/pubmed PY - 2017/1/14/medline KW - Essential oil KW - Limonene KW - MRSA KW - Natural variability KW - Staphylococcus aureus KW - Terebinth SP - 147 EP - 147 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 16 N2 - BACKGROUND: Plant material from different organs of Pistacia terebinthus L., (Anacardiaceae) were collected in Kosovo with aim to analyze the chemical variability of the essential oils among native populations and to test them for potential antibacterial activity against Staphylococcus aureus. METHODS: Essential oils obtained from leaves, pedicels, fruits and galls were analyzed by GC-FID and GC/MS. Minimum inhibitory concentration (MIC) against three clinically relevant strains of S. aureus (NRS385, LAC and UAMS-1) were used to evaluate the antibacterial activity of essential oils. RESULTS: In total, 33 different compounds were identified. The main constituents were α-pinene (12.58-66.29 %), D-limonene (13.95-46.29 %), β-ocimene (0.03-40.49 %), β-pinene (2.63-20.47 %), sabinene (0.00-5.61 %) and (Z)-β-ocimene (0.00-44.85 %). Antibacterial testing of the essential oils against three clinical isolates of S. aureus revealed that seven of the eight samples had some activity at the concentration range tested (0.04-0.512 % v/v). The gall tissues from both sites produced the highest yield of essential oil (3.24 and 6 %), and both exhibited growth inhibitory activity against S. aureus. The most bioactive essential oils, which exhibited MIC90 values ranging from 0.032-0.128 % v/v, obtained from the fruits of the Ura e Shejtë collection site. Likewise, the leaf and pedicel essential oil from the same site was highly active with MIC90 values of 0.064-0.128 and 0.032-0.256 % v/v, respectively. CONCLUSIONS: Principle Component Analyses demonstrated that there is a variation in the chemical composition of essential oil depending on the plant organs from which essential oil are obtained and the geographical origin of the plant populations. The highest variability regarding the chemical composition of essential oil was found between oils obtained from different organs originating from the Prizren site. The MIC90 activity of Pistacia terebinthus was on par or superior compared with Tea Tree Oil control (0.128 % v/v), suggesting that essential oils from this species may have some potential for development as an antibacterial agent for S. aureus infections. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/27229927/Chemical_composition_and_in_vitro_antibacterial_activity_of_Pistacia_terebinthus_essential_oils_derived_from_wild_populations_in_Kosovo_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1135-8 DB - PRIME DP - Unbound Medicine ER -