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All 17 S-locus F-box proteins of the S2 - and S3 -haplotypes of Petunia inflata are assembled into similar SCF complexes with a specific function in self-incompatibility.
Plant J. 2016 09; 87(6):606-16.PJ

Abstract

The collaborative non-self-recognition model for S-RNase-based self-incompatibility predicts that multiple S-locus F-box proteins (SLFs) produced by pollen of a given S-haplotype collectively mediate ubiquitination and degradation of all non-self S-RNases, but not self S-RNases, in the pollen tube, thereby resulting in cross-compatible pollination but self-incompatible pollination. We had previously used pollen extracts containing GFP-fused S2 -SLF1 (SLF1 with an S2 -haplotype) of Petunia inflata for co-immunoprecipitation (Co-IP) and mass spectrometry (MS), and identified PiCUL1-P (a pollen-specific Cullin1), PiSSK1 (a pollen-specific Skp1-like protein) and PiRBX1 (a conventional Rbx1) as components of the SCF(S) (2-) (SLF) (1) complex. Using pollen extracts containing PiSSK1:FLAG:GFP for Co-IP/MS, we identified two additional SLFs (SLF4 and SLF13) that were assembled into SCF(SLF) complexes. As 17 SLF genes (SLF1 to SLF17) have been identified in S2 and S3 pollen, here we examined whether all 17 SLFs are assembled into similar complexes and, if so, whether these complexes are unique to SLFs. We modified the previous Co-IP/MS procedure, including the addition of style extracts from four different S-genotypes to pollen extracts containing PiSSK1:FLAG:GFP, to perform four separate experiments. The results taken together show that all 17 SLFs and an SLF-like protein, SLFLike1 (encoded by an S-locus-linked gene), co-immunoprecipitated with PiSSK1:FLAG:GFP. Moreover, of the 179 other F-box proteins predicted by S2 and S3 pollen transcriptomes, only a pair with 94.9% identity and another pair with 99.7% identity co-immunoprecipitated with PiSSK1:FLAG:GFP. These results suggest that SCF(SLF) complexes have evolved specifically to function in self-incompatibility.

Authors+Show Affiliations

Intercollege Graduate Degree Program in Plant Biology, The Pennsylvania State University, University Park, PA, 16802, USA.Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802, USA.Intercollege Graduate Degree Program in Plant Biology, The Pennsylvania State University, University Park, PA, 16802, USA.Intercollege Graduate Degree Program in Plant Biology, The Pennsylvania State University, University Park, PA, 16802, USA. txk3@psu.edu. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802, USA. txk3@psu.edu.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

27233616

Citation

Li, Shu, et al. "All 17 S-locus F-box Proteins of the S2 - and S3 -haplotypes of Petunia Inflata Are Assembled Into Similar SCF Complexes With a Specific Function in Self-incompatibility." The Plant Journal : for Cell and Molecular Biology, vol. 87, no. 6, 2016, pp. 606-16.
Li S, Williams JS, Sun P, et al. All 17 S-locus F-box proteins of the S2 - and S3 -haplotypes of Petunia inflata are assembled into similar SCF complexes with a specific function in self-incompatibility. Plant J. 2016;87(6):606-16.
Li, S., Williams, J. S., Sun, P., & Kao, T. H. (2016). All 17 S-locus F-box proteins of the S2 - and S3 -haplotypes of Petunia inflata are assembled into similar SCF complexes with a specific function in self-incompatibility. The Plant Journal : for Cell and Molecular Biology, 87(6), 606-16. https://doi.org/10.1111/tpj.13222
Li S, et al. All 17 S-locus F-box Proteins of the S2 - and S3 -haplotypes of Petunia Inflata Are Assembled Into Similar SCF Complexes With a Specific Function in Self-incompatibility. Plant J. 2016;87(6):606-16. PubMed PMID: 27233616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - All 17 S-locus F-box proteins of the S2 - and S3 -haplotypes of Petunia inflata are assembled into similar SCF complexes with a specific function in self-incompatibility. AU - Li,Shu, AU - Williams,Justin S, AU - Sun,Penglin, AU - Kao,Teh-Hui, Y1 - 2016/08/05/ PY - 2016/03/07/received PY - 2016/05/23/accepted PY - 2016/5/29/entrez PY - 2016/5/29/pubmed PY - 2017/10/12/medline KW - Petunia inflata KW - S-locus F-box proteins KW - SCF complexes KW - co-immunoprecipitation KW - mass spectrometry KW - self-incompatibility SP - 606 EP - 16 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 87 IS - 6 N2 - The collaborative non-self-recognition model for S-RNase-based self-incompatibility predicts that multiple S-locus F-box proteins (SLFs) produced by pollen of a given S-haplotype collectively mediate ubiquitination and degradation of all non-self S-RNases, but not self S-RNases, in the pollen tube, thereby resulting in cross-compatible pollination but self-incompatible pollination. We had previously used pollen extracts containing GFP-fused S2 -SLF1 (SLF1 with an S2 -haplotype) of Petunia inflata for co-immunoprecipitation (Co-IP) and mass spectrometry (MS), and identified PiCUL1-P (a pollen-specific Cullin1), PiSSK1 (a pollen-specific Skp1-like protein) and PiRBX1 (a conventional Rbx1) as components of the SCF(S) (2-) (SLF) (1) complex. Using pollen extracts containing PiSSK1:FLAG:GFP for Co-IP/MS, we identified two additional SLFs (SLF4 and SLF13) that were assembled into SCF(SLF) complexes. As 17 SLF genes (SLF1 to SLF17) have been identified in S2 and S3 pollen, here we examined whether all 17 SLFs are assembled into similar complexes and, if so, whether these complexes are unique to SLFs. We modified the previous Co-IP/MS procedure, including the addition of style extracts from four different S-genotypes to pollen extracts containing PiSSK1:FLAG:GFP, to perform four separate experiments. The results taken together show that all 17 SLFs and an SLF-like protein, SLFLike1 (encoded by an S-locus-linked gene), co-immunoprecipitated with PiSSK1:FLAG:GFP. Moreover, of the 179 other F-box proteins predicted by S2 and S3 pollen transcriptomes, only a pair with 94.9% identity and another pair with 99.7% identity co-immunoprecipitated with PiSSK1:FLAG:GFP. These results suggest that SCF(SLF) complexes have evolved specifically to function in self-incompatibility. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/27233616/All_17_S_locus_F_box_proteins_of_the_S2___and_S3__haplotypes_of_Petunia_inflata_are_assembled_into_similar_SCF_complexes_with_a_specific_function_in_self_incompatibility_ DB - PRIME DP - Unbound Medicine ER -