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Validation of Dynamic optical coherence tomography for non-invasive, in vivo microcirculation imaging of the skin.
Microvasc Res. 2016 09; 107:97-105.MR

Abstract

OBJECTIVES

Dynamic optical coherence tomography (D-OCT) is an angiographic variation of OCT that non-invasively provides images of the in vivo microvasculature of the skin by combining conventional OCT images with flow data. The objective of this study was to investigate and report on the D-OCT technique for imaging of the vascular networks in skin as well as to validate the method by comparing the results against already accepted blood flow measuring tools.

METHODS

35 healthy subjects were recruited for the multicentre study, consisting of three experiments set up to examine the vascular blood perfusion during different induced physiologic changes in the blood flow. In order to validate the D-OCT images against existing techniques for blood flow measuring we performed consecutive D-OCT, chromametry and laser speckle contrast imager (LSCI) measurements on identical skin sites in all of the experiments. Blinded observer evaluations were performed in order to evaluate the vascular morphology in the D-OCT images.

RESULTS

The results showed a statistically significant positive correlation between the D-OCT measurements and the LCSI flux measurements (rs=0.494; 95% CI [0.357, 0.615]; p<0.001), and also the redness a* measurements were positively correlated with the D-OCT measurements (r=0.48; 95% CI [0.406, 0.55]). D-OCT was able to reliably image and identify morphologic changes in the vascular network consistent with the induced physiological changes of blood flow.

CONCLUSION

This study has initiated validation of the use of D-OCT for imaging of skin blood flow. Our results showed that D-OCT was able to reliably image and identify changes in the skin vasculature consistent with the induced physiological blood flow changes. These basic findings support the use of D-OCT imaging for in vivo microcirculation imaging of the skin.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Themstrup L
Department of Dermatology, Zealand University Hospital, Roskilde; Health Sciences Faculty, University of Copenhagen, Denmark. Electronic address: lotte.themstrup@gmail.com.
Welzel J
Department of Dermatology and Allergology, General Hospital Augsburg, Augsburg, Germany.
Ciardo S
Department of Dermatology, University of Modena and Reggio Emilia, Modena, Italy.
Kaestle R
Department of Dermatology and Allergology, General Hospital Augsburg, Augsburg, Germany.
Ulrich M
CMB/Collegium Medicum Berlin, Berlin, Germany.
Holmes J
Michelson Diagnostics Ltd., Kent, UK.
Whitehead R
Michelson Diagnostics Ltd., Kent, UK.
Sattler EC
Department of Dermatology and Allergology, Ludwig-Maximilian University, Munich, Germany.
Kindermann N
Department of Dermatology and Allergology, General Hospital Augsburg, Augsburg, Germany.
Pellacani G
Department of Dermatology, University of Modena and Reggio Emilia, Modena, Italy.
Jemec GB
Department of Dermatology, Zealand University Hospital, Roskilde; Health Sciences Faculty, University of Copenhagen, Denmark.

MeSH

AdultBlood Flow VelocityEuropeFemaleHealthy VolunteersHumansLaser-Doppler FlowmetryMaleMicrocirculationMiddle AgedPerfusion ImagingPredictive Value of TestsRegional Blood FlowReproducibility of ResultsSkinTime FactorsTomography, Optical CoherenceYoung Adult

Pub Type(s)

Comparative Study
Journal Article
Multicenter Study
Validation Study

Language

eng

PubMed ID

27235002

Clinical Trial Links

Skin Imaging to Inform Laser Treatments

Citation

Themstrup, L, et al. "Validation of Dynamic Optical Coherence Tomography for Non-invasive, in Vivo Microcirculation Imaging of the Skin." Microvascular Research, vol. 107, 2016, pp. 97-105.
Themstrup L, Welzel J, Ciardo S, et al. Validation of Dynamic optical coherence tomography for non-invasive, in vivo microcirculation imaging of the skin. Microvasc Res. 2016;107:97-105.
Themstrup, L., Welzel, J., Ciardo, S., Kaestle, R., Ulrich, M., Holmes, J., Whitehead, R., Sattler, E. C., Kindermann, N., Pellacani, G., & Jemec, G. B. (2016). Validation of Dynamic optical coherence tomography for non-invasive, in vivo microcirculation imaging of the skin. Microvascular Research, 107, 97-105. https://doi.org/10.1016/j.mvr.2016.05.004
Themstrup L, et al. Validation of Dynamic Optical Coherence Tomography for Non-invasive, in Vivo Microcirculation Imaging of the Skin. Microvasc Res. 2016;107:97-105. PubMed PMID: 27235002.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of Dynamic optical coherence tomography for non-invasive, in vivo microcirculation imaging of the skin. AU - Themstrup,L, AU - Welzel,J, AU - Ciardo,S, AU - Kaestle,R, AU - Ulrich,M, AU - Holmes,J, AU - Whitehead,R, AU - Sattler,E C, AU - Kindermann,N, AU - Pellacani,G, AU - Jemec,G B E, Y1 - 2016/05/25/ PY - 2016/03/22/received PY - 2016/05/13/revised PY - 2016/05/23/accepted PY - 2016/5/29/entrez PY - 2016/5/29/pubmed PY - 2017/10/14/medline KW - Chromametry KW - Dynamic optical coherence tomography KW - Laser speckle contrast imaging KW - Non-invasive imaging KW - OCT angiography KW - Skin microcirculation KW - Speckle variance OCT SP - 97 EP - 105 JF - Microvascular research JO - Microvasc Res VL - 107 N2 - OBJECTIVES: Dynamic optical coherence tomography (D-OCT) is an angiographic variation of OCT that non-invasively provides images of the in vivo microvasculature of the skin by combining conventional OCT images with flow data. The objective of this study was to investigate and report on the D-OCT technique for imaging of the vascular networks in skin as well as to validate the method by comparing the results against already accepted blood flow measuring tools. METHODS: 35 healthy subjects were recruited for the multicentre study, consisting of three experiments set up to examine the vascular blood perfusion during different induced physiologic changes in the blood flow. In order to validate the D-OCT images against existing techniques for blood flow measuring we performed consecutive D-OCT, chromametry and laser speckle contrast imager (LSCI) measurements on identical skin sites in all of the experiments. Blinded observer evaluations were performed in order to evaluate the vascular morphology in the D-OCT images. RESULTS: The results showed a statistically significant positive correlation between the D-OCT measurements and the LCSI flux measurements (rs=0.494; 95% CI [0.357, 0.615]; p<0.001), and also the redness a* measurements were positively correlated with the D-OCT measurements (r=0.48; 95% CI [0.406, 0.55]). D-OCT was able to reliably image and identify morphologic changes in the vascular network consistent with the induced physiological changes of blood flow. CONCLUSION: This study has initiated validation of the use of D-OCT for imaging of skin blood flow. Our results showed that D-OCT was able to reliably image and identify changes in the skin vasculature consistent with the induced physiological blood flow changes. These basic findings support the use of D-OCT imaging for in vivo microcirculation imaging of the skin. SN - 1095-9319 UR - https://www.unboundmedicine.com/medline/citation/27235002/Validation_of_Dynamic_optical_coherence_tomography_for_non_invasive_in_vivo_microcirculation_imaging_of_the_skin_ DB - PRIME DP - Unbound Medicine ER -