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Transciptomic study of mucosal immune, antioxidant and growth related genes and non-specific immune response of common carp (Cyprinus carpio) fed dietary Ferula (Ferula assafoetida).
Fish Shellfish Immunol. 2016 Aug; 55:242-8.FS

Abstract

A 8-weeks feeding trial was conducted to examine the effects of different levels (0, 0.5, 1 and 2%) of dietary Ferula (Ferula assafoetida) on expression of antioxidant enzymes (GSR, GPX and GSTA), immune (TNF-alpha, IL1B, IL- 8 and LYZ) and growth (GH, IGF1 and Ghrl) genes as well as cutaneous mucus and serum non-specific immune response in common carp. The results revealed Ferula significantly increased antioxidant gene expression (GSR and GSTA) in a dose dependent manner (P < 0.05). The expression of immune growth related genes were significantly higher in Ferula fed fish compared control group (P < 0.05). The effects of Ferula on expression of genes was more pronounced in higher doses. Feeding on Ferula supplemented diet remarkably increased skin mucus lysozyme activity (P < 0.05). However, evaluation of mucus total Ig and protease activity revealed no significant difference between control and treated groups (P > 0.05). Regarding non-specific humoral response, serum total Ig, lysozyme and ACH50 showed no remarkable variation between Ferula fed carps and control group (P > 0.05). These results indicated up-regulation of growth and health related genes in Ferula fed common carp. Further studies using pathogen or stress challenge is required to conclude that transcriptional modulation is beneficial in common carp.

Authors+Show Affiliations

Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: rsafari@gau.ac.ir.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: hoseinifar@gau.ac.ir.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27241284

Citation

Safari, Roghieh, et al. "Transciptomic Study of Mucosal Immune, Antioxidant and Growth Related Genes and Non-specific Immune Response of Common Carp (Cyprinus Carpio) Fed Dietary Ferula (Ferula Assafoetida)." Fish & Shellfish Immunology, vol. 55, 2016, pp. 242-8.
Safari R, Hoseinifar SH, Nejadmoghadam S, et al. Transciptomic study of mucosal immune, antioxidant and growth related genes and non-specific immune response of common carp (Cyprinus carpio) fed dietary Ferula (Ferula assafoetida). Fish Shellfish Immunol. 2016;55:242-8.
Safari, R., Hoseinifar, S. H., Nejadmoghadam, S., & Jafar, A. (2016). Transciptomic study of mucosal immune, antioxidant and growth related genes and non-specific immune response of common carp (Cyprinus carpio) fed dietary Ferula (Ferula assafoetida). Fish & Shellfish Immunology, 55, 242-8. https://doi.org/10.1016/j.fsi.2016.05.038
Safari R, et al. Transciptomic Study of Mucosal Immune, Antioxidant and Growth Related Genes and Non-specific Immune Response of Common Carp (Cyprinus Carpio) Fed Dietary Ferula (Ferula Assafoetida). Fish Shellfish Immunol. 2016;55:242-8. PubMed PMID: 27241284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transciptomic study of mucosal immune, antioxidant and growth related genes and non-specific immune response of common carp (Cyprinus carpio) fed dietary Ferula (Ferula assafoetida). AU - Safari,Roghieh, AU - Hoseinifar,Seyed Hossein, AU - Nejadmoghadam,Shabnam, AU - Jafar,Ali, Y1 - 2016/05/27/ PY - 2016/04/22/received PY - 2016/05/23/revised PY - 2016/05/26/accepted PY - 2016/6/1/entrez PY - 2016/6/1/pubmed PY - 2017/2/18/medline KW - Antioxidant defence KW - Ferula (Ferula assafoetida) KW - Immune related gene expression KW - Mucosal immune response KW - Non-specific immunity SP - 242 EP - 8 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 55 N2 - A 8-weeks feeding trial was conducted to examine the effects of different levels (0, 0.5, 1 and 2%) of dietary Ferula (Ferula assafoetida) on expression of antioxidant enzymes (GSR, GPX and GSTA), immune (TNF-alpha, IL1B, IL- 8 and LYZ) and growth (GH, IGF1 and Ghrl) genes as well as cutaneous mucus and serum non-specific immune response in common carp. The results revealed Ferula significantly increased antioxidant gene expression (GSR and GSTA) in a dose dependent manner (P < 0.05). The expression of immune growth related genes were significantly higher in Ferula fed fish compared control group (P < 0.05). The effects of Ferula on expression of genes was more pronounced in higher doses. Feeding on Ferula supplemented diet remarkably increased skin mucus lysozyme activity (P < 0.05). However, evaluation of mucus total Ig and protease activity revealed no significant difference between control and treated groups (P > 0.05). Regarding non-specific humoral response, serum total Ig, lysozyme and ACH50 showed no remarkable variation between Ferula fed carps and control group (P > 0.05). These results indicated up-regulation of growth and health related genes in Ferula fed common carp. Further studies using pathogen or stress challenge is required to conclude that transcriptional modulation is beneficial in common carp. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/27241284/Transciptomic_study_of_mucosal_immune_antioxidant_and_growth_related_genes_and_non_specific_immune_response_of_common_carp__Cyprinus_carpio__fed_dietary_Ferula__Ferula_assafoetida__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(16)30345-X DB - PRIME DP - Unbound Medicine ER -