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Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea.
Microbiol Res. 2016 May-Jun; 186-187:71-80.MR

Abstract

In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n=49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n=22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.

Authors+Show Affiliations

Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, 2092 Tunis, Tunisia; Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, 20133 Milan, Italy.Univ. Manouba, ISBST, BVBGR-LR11ES31, Biotechpole of Sidi Thabet, 2020 Ariana, Tunisia.Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, 2092 Tunis, Tunisia.Univ. Manouba, ISBST, BVBGR-LR11ES31, Biotechpole of Sidi Thabet, 2020 Ariana, Tunisia.Faculty of Science and Technology, Free University of Bozen/Bolzano, Bolzano, Italy.Univ. Manouba, ISBST, BVBGR-LR11ES31, Biotechpole of Sidi Thabet, 2020 Ariana, Tunisia.Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, 20133 Milan, Italy.Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, 20133 Milan, Italy; BESE, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, 2092 Tunis, Tunisia; Univ. Manouba, ISBST, BVBGR-LR11ES31, Biotechpole of Sidi Thabet, 2020 Ariana, Tunisia. Electronic address: cherif.ameur@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27242145

Citation

Ettoumi, Besma, et al. "Diversity, Ecological Distribution and Biotechnological Potential of Actinobacteria Inhabiting Seamounts and Non-seamounts in the Tyrrhenian Sea." Microbiological Research, vol. 186-187, 2016, pp. 71-80.
Ettoumi B, Chouchane H, Guesmi A, et al. Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea. Microbiol Res. 2016;186-187:71-80.
Ettoumi, B., Chouchane, H., Guesmi, A., Mahjoubi, M., Brusetti, L., Neifar, M., Borin, S., Daffonchio, D., & Cherif, A. (2016). Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea. Microbiological Research, 186-187, 71-80. https://doi.org/10.1016/j.micres.2016.03.006
Ettoumi B, et al. Diversity, Ecological Distribution and Biotechnological Potential of Actinobacteria Inhabiting Seamounts and Non-seamounts in the Tyrrhenian Sea. Microbiol Res. 2016 May-Jun;186-187:71-80. PubMed PMID: 27242145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea. AU - Ettoumi,Besma, AU - Chouchane,Habib, AU - Guesmi,Amel, AU - Mahjoubi,Mouna, AU - Brusetti,Lorenzo, AU - Neifar,Mohamed, AU - Borin,Sara, AU - Daffonchio,Daniele, AU - Cherif,Ameur, Y1 - 2016/04/01/ PY - 2015/12/09/received PY - 2016/03/16/revised PY - 2016/03/31/accepted PY - 2016/6/1/entrez PY - 2016/6/1/pubmed PY - 2017/3/31/medline KW - ARISA fingerprinting KW - Actinobacteria KW - BOX-PCR KW - Biosurfactant KW - Deep sea sediments SP - 71 EP - 80 JF - Microbiological research JO - Microbiol Res VL - 186-187 N2 - In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n=49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n=22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche. SN - 1618-0623 UR - https://www.unboundmedicine.com/medline/citation/27242145/Diversity_ecological_distribution_and_biotechnological_potential_of_Actinobacteria_inhabiting_seamounts_and_non_seamounts_in_the_Tyrrhenian_Sea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-5013(16)30081-7 DB - PRIME DP - Unbound Medicine ER -