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Tartrate/tripolyphosphate as co-crosslinker for water soluble chitosan used in protein antigens encapsulation.
Int J Biol Macromol. 2016 Oct; 91:381-93.IJ

Abstract

In drug delivery research, several toxic chemical crosslinkers and non-toxic ionic crosslinkers have been exploited for the synthesis of microparticles from acetic acid soluble chitosan. This paper hypothesized the implementation of sodium potassium tartrate (SPT) as an alternative crosslinker for sodium tripolyphosphate (TPP) and SPT/TPP co-crosslinkers for synthesis of the microparticles using water soluble chitosan (WSC) for encapsulation of Bovine serum albumin (BSA) as a model protein, and Tetanus toxoid (TT) as a model vaccine. The crosslinking was confirmed by FT-IR, SEM with EDS. The XRD entailed molecular dispersion of proteins and thermal analysis confirmed the higher stability of STP/TPP co-crosslinked formulations. The resultant microparticles were exhibiting crosslinking degree (52-67%), entrapment efficiency (72-80%), particle size (0.3-1.7μm), zeta potential (+24 to 46mV) and mucoadhesion (41-68%). The superiority of SPT over TPP was confirmed by higher crosslinking degree and entrapment efficiency. However, co-crosslinking were advantageous in higher regression values for Langmuir adsorption isotherm, slower swelling tendency and extended 30days controlled in-vitro release study. TT release obeyed the Quasi-Fickian diffusion mechanism for single and cocrosslinked formulations. Overall, in crosslinking of chitosan as biological macromolecules, STP/TPP may be alternative for single ionic crosslinked formulations for protein antigen delivery.

Authors+Show Affiliations

Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India.Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India.Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India.Department of Biotechnology, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India.Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune 411028, India.Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune 411028, India.Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune 411028, India.Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India. Electronic address: pdoshi@chem.unipune.ac.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27246374

Citation

Srivastava, Gopal, et al. "Tartrate/tripolyphosphate as Co-crosslinker for Water Soluble Chitosan Used in Protein Antigens Encapsulation." International Journal of Biological Macromolecules, vol. 91, 2016, pp. 381-93.
Srivastava G, Walke S, Dhavale D, et al. Tartrate/tripolyphosphate as co-crosslinker for water soluble chitosan used in protein antigens encapsulation. Int J Biol Macromol. 2016;91:381-93.
Srivastava, G., Walke, S., Dhavale, D., Gade, W., Doshi, J., Kumar, R., Ravetkar, S., & Doshi, P. (2016). Tartrate/tripolyphosphate as co-crosslinker for water soluble chitosan used in protein antigens encapsulation. International Journal of Biological Macromolecules, 91, 381-93. https://doi.org/10.1016/j.ijbiomac.2016.05.099
Srivastava G, et al. Tartrate/tripolyphosphate as Co-crosslinker for Water Soluble Chitosan Used in Protein Antigens Encapsulation. Int J Biol Macromol. 2016;91:381-93. PubMed PMID: 27246374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tartrate/tripolyphosphate as co-crosslinker for water soluble chitosan used in protein antigens encapsulation. AU - Srivastava,Gopal, AU - Walke,Shilratna, AU - Dhavale,Dilip, AU - Gade,Wasudeo, AU - Doshi,Jignesh, AU - Kumar,Rakesh, AU - Ravetkar,Satish, AU - Doshi,Pooja, Y1 - 2016/05/28/ PY - 2015/09/17/received PY - 2016/05/25/revised PY - 2016/05/28/accepted PY - 2016/6/2/entrez PY - 2016/6/2/pubmed PY - 2017/3/3/medline KW - SPT/TPP KW - Water soluble chitosan KW - pH-dependent synthesis SP - 381 EP - 93 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 91 N2 - In drug delivery research, several toxic chemical crosslinkers and non-toxic ionic crosslinkers have been exploited for the synthesis of microparticles from acetic acid soluble chitosan. This paper hypothesized the implementation of sodium potassium tartrate (SPT) as an alternative crosslinker for sodium tripolyphosphate (TPP) and SPT/TPP co-crosslinkers for synthesis of the microparticles using water soluble chitosan (WSC) for encapsulation of Bovine serum albumin (BSA) as a model protein, and Tetanus toxoid (TT) as a model vaccine. The crosslinking was confirmed by FT-IR, SEM with EDS. The XRD entailed molecular dispersion of proteins and thermal analysis confirmed the higher stability of STP/TPP co-crosslinked formulations. The resultant microparticles were exhibiting crosslinking degree (52-67%), entrapment efficiency (72-80%), particle size (0.3-1.7μm), zeta potential (+24 to 46mV) and mucoadhesion (41-68%). The superiority of SPT over TPP was confirmed by higher crosslinking degree and entrapment efficiency. However, co-crosslinking were advantageous in higher regression values for Langmuir adsorption isotherm, slower swelling tendency and extended 30days controlled in-vitro release study. TT release obeyed the Quasi-Fickian diffusion mechanism for single and cocrosslinked formulations. Overall, in crosslinking of chitosan as biological macromolecules, STP/TPP may be alternative for single ionic crosslinked formulations for protein antigen delivery. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/27246374/Tartrate/tripolyphosphate_as_co_crosslinker_for_water_soluble_chitosan_used_in_protein_antigens_encapsulation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(16)30513-X DB - PRIME DP - Unbound Medicine ER -