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Mesenchymal Stem Cells from Human Extra Ocular Muscle Harbor Neuroectodermal Differentiation Potential.
PLoS One 2016; 11(6):e0156697Plos

Abstract

Mesenchymal stem cells (MSC) have been proposed as suitable candidates for cell therapy for neurological disorderssince they exhibit good neuronal differentiation capacity. However, for better therapeutic outcomes, it is necessary to isolate MSC from a suitable tissue sourcethat posses high neuronal differentiation. In this context, we isolated MSC from extra ocular muscle (EOM) tissue and tested the in vitro neuronal differentiation potential. In the current study, EOM tissue derived MSC were characterized and compared with bone marrow derived MSC. We found that EOM derived MSC proliferated as a monolayer and showed similarities in morphology, growth properties and cell surface marker expression with bone marrow derived MSC and expressed high levels of NES, OCT4, NANOG and SOX2 in its undifferentiated state. They also expressed embryonic cell surface marker SSEA4 and their intracellular mitochondrial distribution pattern was similar to that of multipotent stem cells. Although EOM derived MSC differentiated readily into adipocytes, osteocytes and chondrocytes, they differentiated more efficiently into neuroectodermal cells. The differentiation into neuroectodermal cellswas confirmed by the expression of neuronal markers NGFR and MAP2B. Thus, EOM derived MSC might be good candidates for stem cell based therapies for treating neurodegenerative diseases.

Authors+Show Affiliations

Stem Cell and Cancer Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.Stem Cell and Cancer Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.Department of Strabismus, Sri Sankaradeva Nethralaya Hospital, Guwahati, Assam, India.Department of Hematology, Gauhati Medical College and Hospital, Guwahati, Assam, India.Stem Cell and Cancer Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27248788

Citation

Mawrie, Darilang, et al. "Mesenchymal Stem Cells From Human Extra Ocular Muscle Harbor Neuroectodermal Differentiation Potential." PloS One, vol. 11, no. 6, 2016, pp. e0156697.
Mawrie D, Kumar A, Magdalene D, et al. Mesenchymal Stem Cells from Human Extra Ocular Muscle Harbor Neuroectodermal Differentiation Potential. PLoS ONE. 2016;11(6):e0156697.
Mawrie, D., Kumar, A., Magdalene, D., Bhattacharyya, J., & Jaganathan, B. G. (2016). Mesenchymal Stem Cells from Human Extra Ocular Muscle Harbor Neuroectodermal Differentiation Potential. PloS One, 11(6), pp. e0156697. doi:10.1371/journal.pone.0156697.
Mawrie D, et al. Mesenchymal Stem Cells From Human Extra Ocular Muscle Harbor Neuroectodermal Differentiation Potential. PLoS ONE. 2016;11(6):e0156697. PubMed PMID: 27248788.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mesenchymal Stem Cells from Human Extra Ocular Muscle Harbor Neuroectodermal Differentiation Potential. AU - Mawrie,Darilang, AU - Kumar,Atul, AU - Magdalene,Damaris, AU - Bhattacharyya,Jina, AU - Jaganathan,Bithiah Grace, Y1 - 2016/06/01/ PY - 2015/12/30/received PY - 2016/05/18/accepted PY - 2016/6/2/entrez PY - 2016/6/2/pubmed PY - 2017/7/19/medline SP - e0156697 EP - e0156697 JF - PloS one JO - PLoS ONE VL - 11 IS - 6 N2 - Mesenchymal stem cells (MSC) have been proposed as suitable candidates for cell therapy for neurological disorderssince they exhibit good neuronal differentiation capacity. However, for better therapeutic outcomes, it is necessary to isolate MSC from a suitable tissue sourcethat posses high neuronal differentiation. In this context, we isolated MSC from extra ocular muscle (EOM) tissue and tested the in vitro neuronal differentiation potential. In the current study, EOM tissue derived MSC were characterized and compared with bone marrow derived MSC. We found that EOM derived MSC proliferated as a monolayer and showed similarities in morphology, growth properties and cell surface marker expression with bone marrow derived MSC and expressed high levels of NES, OCT4, NANOG and SOX2 in its undifferentiated state. They also expressed embryonic cell surface marker SSEA4 and their intracellular mitochondrial distribution pattern was similar to that of multipotent stem cells. Although EOM derived MSC differentiated readily into adipocytes, osteocytes and chondrocytes, they differentiated more efficiently into neuroectodermal cells. The differentiation into neuroectodermal cellswas confirmed by the expression of neuronal markers NGFR and MAP2B. Thus, EOM derived MSC might be good candidates for stem cell based therapies for treating neurodegenerative diseases. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27248788/Mesenchymal_Stem_Cells_from_Human_Extra_Ocular_Muscle_Harbor_Neuroectodermal_Differentiation_Potential_ L2 - http://dx.plos.org/10.1371/journal.pone.0156697 DB - PRIME DP - Unbound Medicine ER -