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Genotoxic effect of a binary mixture of dicamba- and glyphosate-based commercial herbicide formulations on Rhinella arenarum (Hensel, 1867) (Anura, Bufonidae) late-stage larvae.
Environ Sci Pollut Res Int. 2016 Sep; 23(17):17811-21.ES

Abstract

The acute toxicity of two herbicide formulations, namely, the 57.71 % dicamba (DIC)-based Banvel(®) and the 48 % glyphosate (GLY)-based Credit(®), alone as well as the binary mixture of these herbicides was evaluated on late-stage Rhinella arenarum larvae (stage 36) exposed under laboratory conditions. Mortality was used as an endpoint for determining acute lethal effects, whereas the single-cell gel electrophoresis (SCGE) assay was employed as genotoxic endpoint to study sublethal effects. Lethality studies revealed LC5096 h values of 358.44 and 78.18 mg L(-1) DIC and GLY for Banvel(®) and Credit(®), respectively. SCGE assay revealed, after exposure for 96 h to either 5 and 10 % of the Banvel(®) LC5096 h concentration or 5 and 10 % of the Credit(®) LC5096 h concentration, an equal significant increase of the genetic damage index (GDI) regardless of the concentration of the herbicide assayed. The binary mixtures of 5 % Banvel(®) plus 5 % Credit(®) LC5096 h concentrations and 10 % Banvel(®) plus 10 % Credit(®) LC5096 h concentrations induced equivalent significant increases in the GDI in regard to GDI values from late-stage larvae exposed only to Banvel(®) or Credit(®). This study represents the first experimental evidence of acute lethal and sublethal effects exerted by DIC on the species, as well as the induction of primary DNA breaks by this herbicide in amphibians. Finally, a synergistic effect of the mixture of GLY and DIC on the induction of primary DNA breaks on circulating blood cells of R. arenarum late-stage larvae could be demonstrated.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nro. 3 (esq. 120), B1904AMA, La Plata, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nro. 3 (esq. 120), B1904AMA, La Plata, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nro. 3 (esq. 120), B1904AMA, La Plata, Argentina. marcelo.larramendy@gmail.com. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. marcelo.larramendy@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27250090

Citation

Soloneski, Sonia, et al. "Genotoxic Effect of a Binary Mixture of Dicamba- and Glyphosate-based Commercial Herbicide Formulations On Rhinella Arenarum (Hensel, 1867) (Anura, Bufonidae) Late-stage Larvae." Environmental Science and Pollution Research International, vol. 23, no. 17, 2016, pp. 17811-21.
Soloneski S, Ruiz de Arcaute C, Larramendy ML. Genotoxic effect of a binary mixture of dicamba- and glyphosate-based commercial herbicide formulations on Rhinella arenarum (Hensel, 1867) (Anura, Bufonidae) late-stage larvae. Environ Sci Pollut Res Int. 2016;23(17):17811-21.
Soloneski, S., Ruiz de Arcaute, C., & Larramendy, M. L. (2016). Genotoxic effect of a binary mixture of dicamba- and glyphosate-based commercial herbicide formulations on Rhinella arenarum (Hensel, 1867) (Anura, Bufonidae) late-stage larvae. Environmental Science and Pollution Research International, 23(17), 17811-21. https://doi.org/10.1007/s11356-016-6992-7
Soloneski S, Ruiz de Arcaute C, Larramendy ML. Genotoxic Effect of a Binary Mixture of Dicamba- and Glyphosate-based Commercial Herbicide Formulations On Rhinella Arenarum (Hensel, 1867) (Anura, Bufonidae) Late-stage Larvae. Environ Sci Pollut Res Int. 2016;23(17):17811-21. PubMed PMID: 27250090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic effect of a binary mixture of dicamba- and glyphosate-based commercial herbicide formulations on Rhinella arenarum (Hensel, 1867) (Anura, Bufonidae) late-stage larvae. AU - Soloneski,Sonia, AU - Ruiz de Arcaute,Celeste, AU - Larramendy,Marcelo L, Y1 - 2016/06/01/ PY - 2016/01/14/received PY - 2016/05/26/accepted PY - 2016/6/3/entrez PY - 2016/6/3/pubmed PY - 2017/4/21/medline KW - Amphibians KW - Dicamba-based formulations KW - Glyphosate-based formulations KW - Lethal effects KW - SCGE assay SP - 17811 EP - 21 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 23 IS - 17 N2 - The acute toxicity of two herbicide formulations, namely, the 57.71 % dicamba (DIC)-based Banvel(®) and the 48 % glyphosate (GLY)-based Credit(®), alone as well as the binary mixture of these herbicides was evaluated on late-stage Rhinella arenarum larvae (stage 36) exposed under laboratory conditions. Mortality was used as an endpoint for determining acute lethal effects, whereas the single-cell gel electrophoresis (SCGE) assay was employed as genotoxic endpoint to study sublethal effects. Lethality studies revealed LC5096 h values of 358.44 and 78.18 mg L(-1) DIC and GLY for Banvel(®) and Credit(®), respectively. SCGE assay revealed, after exposure for 96 h to either 5 and 10 % of the Banvel(®) LC5096 h concentration or 5 and 10 % of the Credit(®) LC5096 h concentration, an equal significant increase of the genetic damage index (GDI) regardless of the concentration of the herbicide assayed. The binary mixtures of 5 % Banvel(®) plus 5 % Credit(®) LC5096 h concentrations and 10 % Banvel(®) plus 10 % Credit(®) LC5096 h concentrations induced equivalent significant increases in the GDI in regard to GDI values from late-stage larvae exposed only to Banvel(®) or Credit(®). This study represents the first experimental evidence of acute lethal and sublethal effects exerted by DIC on the species, as well as the induction of primary DNA breaks by this herbicide in amphibians. Finally, a synergistic effect of the mixture of GLY and DIC on the induction of primary DNA breaks on circulating blood cells of R. arenarum late-stage larvae could be demonstrated. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/27250090/Genotoxic_effect_of_a_binary_mixture_of_dicamba__and_glyphosate_based_commercial_herbicide_formulations_on_Rhinella_arenarum__Hensel_1867___Anura_Bufonidae__late_stage_larvae_ L2 - https://dx.doi.org/10.1007/s11356-016-6992-7 DB - PRIME DP - Unbound Medicine ER -