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Increased concentration of 8-hydroxy-2'-deoxyguanosine in follicular fluid of infertile women with endometriosis.
Cell Tissue Res 2016; 366(1):231-42CT

Abstract

Impaired oocyte quality and oxidative stress might be involved in the pathogenesis of endometriosis-related infertility. To improve our understanding of the role of oxidative stress in this condition, we compare eight oxidative stress markers from each stage, including the simultaneous analysis of lipids, proteins and DNA damage, in the serum and follicular fluid of infertile women with endometriosis and infertile controls undergoing controlled ovarian stimulation for intracytoplasmic sperm injection. In total, 87 serum samples (43 with endometriosis, 44 controls) and 61 follicular fluid samples (29 with endometriosis, 32 controls) free of blood contamination upon visual inspection and presenting granulosa cells alone or granulosa cells plus a retrieved mature oocyte were collected on the day of oocyte retrieval. Total hydroperoxides, malondialdehyde, advanced oxidation protein products, glutathione, superoxide dismutase (SOD) and total antioxidant capacity (TAC) were determined by spectrophotometry, vitamin E by high-performance liquid chromatography and 8-hydroxy-2'-deoxyguanosine (8OHdG) by enzyme-linked immunosorbent assay. The endometriosis group showed higher serum concentrations of glutathione and SOD, lower serum concentrations of TAC and higher follicular concentrations of 8OHdG and vitamin E compared with infertile controls. These data indicate both systemic and follicular oxidative stress in infertile patients with endometriosis. For the first time, we demonstrate the presence of oxidative DNA damage, represented by higher 8OHdG concentrations in the follicular microenvironment of these patients, possibly related to compromised oocyte quality and associated with the pathogenesis of endometriosis-related infertility.

Authors+Show Affiliations

Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900. michi.dabroi@gmail.com.Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900.Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900.Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900.Nutrition and Metabolism Laboratory, Department of Internal Medicine, Ribeirão Preto School of Medicine, University of Sao Paulo, Ribeirão Preto, SP, Brazil.Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27250533

Citation

Da Broi, Michele G., et al. "Increased Concentration of 8-hydroxy-2'-deoxyguanosine in Follicular Fluid of Infertile Women With Endometriosis." Cell and Tissue Research, vol. 366, no. 1, 2016, pp. 231-42.
Da Broi MG, de Albuquerque FO, de Andrade AZ, et al. Increased concentration of 8-hydroxy-2'-deoxyguanosine in follicular fluid of infertile women with endometriosis. Cell Tissue Res. 2016;366(1):231-42.
Da Broi, M. G., de Albuquerque, F. O., de Andrade, A. Z., Cardoso, R. L., Jordão Junior, A. A., & Navarro, P. A. (2016). Increased concentration of 8-hydroxy-2'-deoxyguanosine in follicular fluid of infertile women with endometriosis. Cell and Tissue Research, 366(1), pp. 231-42. doi:10.1007/s00441-016-2428-4.
Da Broi MG, et al. Increased Concentration of 8-hydroxy-2'-deoxyguanosine in Follicular Fluid of Infertile Women With Endometriosis. Cell Tissue Res. 2016;366(1):231-42. PubMed PMID: 27250533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased concentration of 8-hydroxy-2'-deoxyguanosine in follicular fluid of infertile women with endometriosis. AU - Da Broi,Michele G, AU - de Albuquerque,Felipe O, AU - de Andrade,Aline Z, AU - Cardoso,Rafaela L, AU - Jordão Junior,Alceu A, AU - Navarro,Paula A, Y1 - 2016/06/01/ PY - 2015/09/23/received PY - 2016/04/29/accepted PY - 2016/6/3/entrez PY - 2016/6/3/pubmed PY - 2017/5/17/medline KW - 8-hydroxy-2′-deoxyguanosine KW - Endometriosis KW - Follicular fluid KW - Infertility KW - Oxidative stress SP - 231 EP - 42 JF - Cell and tissue research JO - Cell Tissue Res. VL - 366 IS - 1 N2 - Impaired oocyte quality and oxidative stress might be involved in the pathogenesis of endometriosis-related infertility. To improve our understanding of the role of oxidative stress in this condition, we compare eight oxidative stress markers from each stage, including the simultaneous analysis of lipids, proteins and DNA damage, in the serum and follicular fluid of infertile women with endometriosis and infertile controls undergoing controlled ovarian stimulation for intracytoplasmic sperm injection. In total, 87 serum samples (43 with endometriosis, 44 controls) and 61 follicular fluid samples (29 with endometriosis, 32 controls) free of blood contamination upon visual inspection and presenting granulosa cells alone or granulosa cells plus a retrieved mature oocyte were collected on the day of oocyte retrieval. Total hydroperoxides, malondialdehyde, advanced oxidation protein products, glutathione, superoxide dismutase (SOD) and total antioxidant capacity (TAC) were determined by spectrophotometry, vitamin E by high-performance liquid chromatography and 8-hydroxy-2'-deoxyguanosine (8OHdG) by enzyme-linked immunosorbent assay. The endometriosis group showed higher serum concentrations of glutathione and SOD, lower serum concentrations of TAC and higher follicular concentrations of 8OHdG and vitamin E compared with infertile controls. These data indicate both systemic and follicular oxidative stress in infertile patients with endometriosis. For the first time, we demonstrate the presence of oxidative DNA damage, represented by higher 8OHdG concentrations in the follicular microenvironment of these patients, possibly related to compromised oocyte quality and associated with the pathogenesis of endometriosis-related infertility. SN - 1432-0878 UR - https://www.unboundmedicine.com/medline/citation/27250533/Increased_concentration_of_8_hydroxy_2'_deoxyguanosine_in_follicular_fluid_of_infertile_women_with_endometriosis_ L2 - https://dx.doi.org/10.1007/s00441-016-2428-4 DB - PRIME DP - Unbound Medicine ER -