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A homogeneous and "off-on" fluorescence aptamer-based assay for chloramphenicol using vesicle quantum dot-gold colloid composite probes.
Anal Chim Acta. 2016 Jul 27; 929:49-55.AC

Abstract

In this work, a novel homogeneous and signal "off-on" aptamer based fluorescence assay was successfully developed to detect chloramphenicol (CAP) residues in food based on the fluorescence resonance energy transfer (FRET). The vesicle nanotracer was prepared through labeling single stranded DNA binding protein (SSB) on limposome-CdSe/ZnS quantum dot (SSB/L-QD) complexes. It was worth mentioning that the signal tracer (SSB/L-QD) with vesicle shape, which was fabricated being encapsulated with a number of quantum dots and SSB. The nanotracer has excellent signal amplification effects. The vesicle composite probe was formed by combining aptamer labeled nano-gold (Au-Apt) and SSB/L-QD. Which based on SSB's specific affinity towards aptamer. This probe can't emit fluoresce which is in "off" state because the signal from SSB/L-QD as donor can be quenched by the Au-aptas acceptor. When CAP was added in the composite probe solution, the aptamer on the Au-Apt can be preferentially bounded with CAP then release from the composite probe, which can turn the "off" signal of SSB/L-QD tracer into "on" state. The assay indicates excellent linear response to CAP from 0.001 nM to 10 nM and detection limit down to 0.3 pM. The vesicle probes with size of 88 nm have strong signal amplification. Because a larger number of QDs can be labeled inside the double phosphorus lipid membrane. Besides, it was employed to detect CAP residues in the milk samples with results being agreed well with those from ELISA, verifying its accuracy and reliability.

Authors+Show Affiliations

State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.Key Laboratory of Asymmetric Synthesis and Chirotechnology of Sichuan Province, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, PR China; University of Chinese Academy of Sciences, Beijing 10049, PR China.State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: ganning@nbu.edu.cn.State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: caoyuting@nbu.edu.cn.State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.Faculty of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210000, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27251948

Citation

Miao, Yang-Bao, et al. "A Homogeneous and "off-on" Fluorescence Aptamer-based Assay for Chloramphenicol Using Vesicle Quantum Dot-gold Colloid Composite Probes." Analytica Chimica Acta, vol. 929, 2016, pp. 49-55.
Miao YB, Ren HX, Gan N, et al. A homogeneous and "off-on" fluorescence aptamer-based assay for chloramphenicol using vesicle quantum dot-gold colloid composite probes. Anal Chim Acta. 2016;929:49-55.
Miao, Y. B., Ren, H. X., Gan, N., Zhou, Y., Cao, Y., Li, T., & Chen, Y. (2016). A homogeneous and "off-on" fluorescence aptamer-based assay for chloramphenicol using vesicle quantum dot-gold colloid composite probes. Analytica Chimica Acta, 929, 49-55. https://doi.org/10.1016/j.aca.2016.04.060
Miao YB, et al. A Homogeneous and "off-on" Fluorescence Aptamer-based Assay for Chloramphenicol Using Vesicle Quantum Dot-gold Colloid Composite Probes. Anal Chim Acta. 2016 Jul 27;929:49-55. PubMed PMID: 27251948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A homogeneous and "off-on" fluorescence aptamer-based assay for chloramphenicol using vesicle quantum dot-gold colloid composite probes. AU - Miao,Yang-Bao, AU - Ren,Hong-Xia, AU - Gan,Ning, AU - Zhou,You, AU - Cao,Yuting, AU - Li,Tianhua, AU - Chen,Yinji, Y1 - 2016/05/04/ PY - 2016/03/25/received PY - 2016/04/26/revised PY - 2016/04/30/accepted PY - 2016/6/3/entrez PY - 2016/6/3/pubmed PY - 2018/2/8/medline KW - Chloramphenicol KW - Fluorescence aptasensor KW - Homogeneous assay KW - Off–on KW - liposome−quantum dot SP - 49 EP - 55 JF - Analytica chimica acta JO - Anal Chim Acta VL - 929 N2 - In this work, a novel homogeneous and signal "off-on" aptamer based fluorescence assay was successfully developed to detect chloramphenicol (CAP) residues in food based on the fluorescence resonance energy transfer (FRET). The vesicle nanotracer was prepared through labeling single stranded DNA binding protein (SSB) on limposome-CdSe/ZnS quantum dot (SSB/L-QD) complexes. It was worth mentioning that the signal tracer (SSB/L-QD) with vesicle shape, which was fabricated being encapsulated with a number of quantum dots and SSB. The nanotracer has excellent signal amplification effects. The vesicle composite probe was formed by combining aptamer labeled nano-gold (Au-Apt) and SSB/L-QD. Which based on SSB's specific affinity towards aptamer. This probe can't emit fluoresce which is in "off" state because the signal from SSB/L-QD as donor can be quenched by the Au-aptas acceptor. When CAP was added in the composite probe solution, the aptamer on the Au-Apt can be preferentially bounded with CAP then release from the composite probe, which can turn the "off" signal of SSB/L-QD tracer into "on" state. The assay indicates excellent linear response to CAP from 0.001 nM to 10 nM and detection limit down to 0.3 pM. The vesicle probes with size of 88 nm have strong signal amplification. Because a larger number of QDs can be labeled inside the double phosphorus lipid membrane. Besides, it was employed to detect CAP residues in the milk samples with results being agreed well with those from ELISA, verifying its accuracy and reliability. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/27251948/A_homogeneous_and_"off_on"_fluorescence_aptamer_based_assay_for_chloramphenicol_using_vesicle_quantum_dot_gold_colloid_composite_probes_ DB - PRIME DP - Unbound Medicine ER -