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Remarkable preservation of Ca(2+) homeostasis and inhibition of apoptosis contribute to anti-muscle atrophy effect in hibernating Daurian ground squirrels.

Abstract

The underlying mechanisms that hibernators deviated from muscle atrophy during prolonged hibernating inactivity remain elusive. This study tested the hypothesis that the maintenance of intracellular Ca(2+) homeostasis and inhibition of apoptosis would be responsible for preventing muscle atrophy in hibernating Daurian ground squirrels. The results showed that intracellular Ca(2+) homeostasis was maintained in soleus and extensor digitorum longus (EDL) in hibernation and post-hibernation, while cytosolic Ca(2+) was overloaded in gastrocnemius (GAS) in hibernation with a recovery in post-hibernation. The Ca(2+) overload was also observed in interbout arousals in all three type muscles. Besides, the Bax/Bcl-2 ratio was unchanged in transcriptional level among pre-hibernation, hibernation and interbout arousals, and reduced to a minimum in post-hibernation. Furthermore, the Bax/Bcl-2 ratio in protein level was reduced in hibernation but recovered in interbout arousals. Although cytochrome C was increased in GAS and EDL in post-hibernation, no apoptosis was observed by TUNEL assay. These findings suggested that the intracellular Ca(2+) homeostasis in hibernation might be regulated by the cytosolic Ca(2+) overload during interbout arousals, which were likely responsible for preventing muscle atrophy via inhibition of apoptosis. Moreover, the muscle-specificity indicated that the different mechanisms against disuse-induced atrophy might be involved in different muscles in hibernation.

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  • Authors+Show Affiliations

    ,

    Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.

    ,

    National Research Center for Veterinary Medicine, Luo Yang 471003, China.

    ,

    Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.

    ,

    Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.

    ,

    Shaanxi Institute of International Trade and Commerce, Xian Yang 712046, China.

    ,

    Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.

    Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.

    Source

    Scientific reports 6: 2016 06 03 pg 27020

    MeSH

    Animals
    Apoptosis
    Body Weight Maintenance
    Calcium
    Cytochromes c
    DNA Fragmentation
    Female
    Gene Expression
    Hibernation
    Homeostasis
    Male
    Muscle, Skeletal
    Muscular Atrophy
    Myosin Heavy Chains
    Proto-Oncogene Proteins c-bcl-2
    Sciuridae
    bcl-2-Associated X Protein

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    27256167

    Citation

    Fu, Weiwei, et al. "Remarkable Preservation of Ca(2+) Homeostasis and Inhibition of Apoptosis Contribute to Anti-muscle Atrophy Effect in Hibernating Daurian Ground Squirrels." Scientific Reports, vol. 6, 2016, p. 27020.
    Fu W, Hu H, Dang K, et al. Remarkable preservation of Ca(2+) homeostasis and inhibition of apoptosis contribute to anti-muscle atrophy effect in hibernating Daurian ground squirrels. Sci Rep. 2016;6:27020.
    Fu, W., Hu, H., Dang, K., Chang, H., Du, B., Wu, X., & Gao, Y. (2016). Remarkable preservation of Ca(2+) homeostasis and inhibition of apoptosis contribute to anti-muscle atrophy effect in hibernating Daurian ground squirrels. Scientific Reports, 6, p. 27020. doi:10.1038/srep27020.
    Fu W, et al. Remarkable Preservation of Ca(2+) Homeostasis and Inhibition of Apoptosis Contribute to Anti-muscle Atrophy Effect in Hibernating Daurian Ground Squirrels. Sci Rep. 2016 06 3;6:27020. PubMed PMID: 27256167.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Remarkable preservation of Ca(2+) homeostasis and inhibition of apoptosis contribute to anti-muscle atrophy effect in hibernating Daurian ground squirrels. AU - Fu,Weiwei, AU - Hu,Huanxin, AU - Dang,Kai, AU - Chang,Hui, AU - Du,Bei, AU - Wu,Xue, AU - Gao,Yunfang, Y1 - 2016/06/03/ PY - 2016/01/14/received PY - 2016/05/12/accepted PY - 2016/6/4/entrez PY - 2016/6/4/pubmed PY - 2018/4/17/medline SP - 27020 EP - 27020 JF - Scientific reports JO - Sci Rep VL - 6 N2 - The underlying mechanisms that hibernators deviated from muscle atrophy during prolonged hibernating inactivity remain elusive. This study tested the hypothesis that the maintenance of intracellular Ca(2+) homeostasis and inhibition of apoptosis would be responsible for preventing muscle atrophy in hibernating Daurian ground squirrels. The results showed that intracellular Ca(2+) homeostasis was maintained in soleus and extensor digitorum longus (EDL) in hibernation and post-hibernation, while cytosolic Ca(2+) was overloaded in gastrocnemius (GAS) in hibernation with a recovery in post-hibernation. The Ca(2+) overload was also observed in interbout arousals in all three type muscles. Besides, the Bax/Bcl-2 ratio was unchanged in transcriptional level among pre-hibernation, hibernation and interbout arousals, and reduced to a minimum in post-hibernation. Furthermore, the Bax/Bcl-2 ratio in protein level was reduced in hibernation but recovered in interbout arousals. Although cytochrome C was increased in GAS and EDL in post-hibernation, no apoptosis was observed by TUNEL assay. These findings suggested that the intracellular Ca(2+) homeostasis in hibernation might be regulated by the cytosolic Ca(2+) overload during interbout arousals, which were likely responsible for preventing muscle atrophy via inhibition of apoptosis. Moreover, the muscle-specificity indicated that the different mechanisms against disuse-induced atrophy might be involved in different muscles in hibernation. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27256167/Remarkable_preservation_of_Ca_2+__homeostasis_and_inhibition_of_apoptosis_contribute_to_anti_muscle_atrophy_effect_in_hibernating_Daurian_ground_squirrels_ L2 - http://dx.doi.org/10.1038/srep27020 DB - PRIME DP - Unbound Medicine ER -