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Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity.
FASEB J. 2016 09; 30(9):3117-23.FJ

Abstract

In humans, insulin sensitivity varies according to time of day, with decreased values in the evening and at night. Mechanisms responsible for the diurnal variation in insulin sensitivity are unclear. We investigated whether human adipose tissue (AT) expresses intrinsic circadian rhythms in insulin sensitivity that could contribute to this phenomenon. Subcutaneous and visceral AT biopsies were obtained from extremely obese participants (body mass index, 41.8 ± 6.3 kg/m(2); 46 ± 11 y) during gastric-bypass surgery. To assess the rhythm in insulin signaling, AKT phosphorylation was determined every 4 h over 24 h in vitro in response to different insulin concentrations (0, 1, 10, and 100 nM). Data revealed that subcutaneous AT exhibited robust circadian rhythms in insulin signaling (P < 0.00001). Insulin sensitivity reached its maximum (acrophase) around noon, being 54% higher than during midnight (P = 0.009). The amplitude of the rhythm was positively correlated with in vivo sleep duration (r = 0.53; P = 0.023) and negatively correlated with in vivo bedtime (r = -0.54; P = 0.020). No circadian rhythms were detected in visceral AT (P = 0.643). Here, we demonstrate the relevance of the time of the day for how sensitive AT is to the effects of insulin. Subcutaneous AT shows an endogenous circadian rhythm in insulin sensitivity that could provide an underlying mechanism for the daily rhythm in systemic insulin sensitivity.-Carrasco-Benso, M. P., Rivero-Gutierrez, B., Lopez-Minguez, J., Anzola, A., Diez-Noguera, A., Madrid, J. A., Lujan, J. A., Martínez-Augustin, O., Scheer, F. A. J. L., Garaulet, M. Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity.

Authors+Show Affiliations

Department of Physiology, Faculty of Biology, Biomedical Research Institute of Murcia (IMIB)-Arrixaca, University of Murcia, Murcia, Spain;Department of Biochemistry and Molecular Biology II, Biomedical Research Networking Center in Hepatic and Digestive Diseases (CIBEREHD), School of Pharmacy, Instituto de Investigación Biosanitaria de Granada, University of Granada, Granada, Spain;Department of Physiology, Faculty of Biology, Biomedical Research Institute of Murcia (IMIB)-Arrixaca, University of Murcia, Murcia, Spain;Department of Biochemistry and Molecular Biology II, Biomedical Research Networking Center in Hepatic and Digestive Diseases (CIBEREHD), School of Pharmacy, Instituto de Investigación Biosanitaria de Granada, University of Granada, Granada, Spain;Department of Physiology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain;Department of Physiology, Faculty of Biology, Biomedical Research Institute of Murcia (IMIB)-Arrixaca, University of Murcia, Murcia, Spain;General Surgery Service, University Hospital Virgen de la Arrixaca, Murcia, Spain;Department of Biochemistry and Molecular Biology II, Biomedical Research Networking Center in Hepatic and Digestive Diseases (CIBEREHD), School of Pharmacy, Instituto de Investigación Biosanitaria de Granada, University of Granada, Granada, Spain;Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Boston, Massachusetts, USA; and Division of Sleep Medicine, Harvard Medical School, Boston, Massachusetts, USA fscheer@bwh.harvard.edu.Department of Physiology, Faculty of Biology, Biomedical Research Institute of Murcia (IMIB)-Arrixaca, University of Murcia, Murcia, Spain; garaulet@um.es.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27256623

Citation

Carrasco-Benso, Maria P., et al. "Human Adipose Tissue Expresses Intrinsic Circadian Rhythm in Insulin Sensitivity." FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 30, no. 9, 2016, pp. 3117-23.
Carrasco-Benso MP, Rivero-Gutierrez B, Lopez-Minguez J, et al. Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity. FASEB J. 2016;30(9):3117-23.
Carrasco-Benso, M. P., Rivero-Gutierrez, B., Lopez-Minguez, J., Anzola, A., Diez-Noguera, A., Madrid, J. A., Lujan, J. A., Martínez-Augustin, O., Scheer, F. A., & Garaulet, M. (2016). Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 30(9), 3117-23. https://doi.org/10.1096/fj.201600269RR
Carrasco-Benso MP, et al. Human Adipose Tissue Expresses Intrinsic Circadian Rhythm in Insulin Sensitivity. FASEB J. 2016;30(9):3117-23. PubMed PMID: 27256623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity. AU - Carrasco-Benso,Maria P, AU - Rivero-Gutierrez,Belen, AU - Lopez-Minguez,Jesus, AU - Anzola,Andrea, AU - Diez-Noguera,Antoni, AU - Madrid,Juan A, AU - Lujan,Juan A, AU - Martínez-Augustin,Olga, AU - Scheer,Frank A J L, AU - Garaulet,Marta, Y1 - 2016/06/02/ PY - 2016/01/27/received PY - 2016/05/19/accepted PY - 2016/6/4/entrez PY - 2016/6/4/pubmed PY - 2017/8/23/medline KW - diabetes KW - glucose tolerance KW - obesity SP - 3117 EP - 23 JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JO - FASEB J. VL - 30 IS - 9 N2 - In humans, insulin sensitivity varies according to time of day, with decreased values in the evening and at night. Mechanisms responsible for the diurnal variation in insulin sensitivity are unclear. We investigated whether human adipose tissue (AT) expresses intrinsic circadian rhythms in insulin sensitivity that could contribute to this phenomenon. Subcutaneous and visceral AT biopsies were obtained from extremely obese participants (body mass index, 41.8 ± 6.3 kg/m(2); 46 ± 11 y) during gastric-bypass surgery. To assess the rhythm in insulin signaling, AKT phosphorylation was determined every 4 h over 24 h in vitro in response to different insulin concentrations (0, 1, 10, and 100 nM). Data revealed that subcutaneous AT exhibited robust circadian rhythms in insulin signaling (P < 0.00001). Insulin sensitivity reached its maximum (acrophase) around noon, being 54% higher than during midnight (P = 0.009). The amplitude of the rhythm was positively correlated with in vivo sleep duration (r = 0.53; P = 0.023) and negatively correlated with in vivo bedtime (r = -0.54; P = 0.020). No circadian rhythms were detected in visceral AT (P = 0.643). Here, we demonstrate the relevance of the time of the day for how sensitive AT is to the effects of insulin. Subcutaneous AT shows an endogenous circadian rhythm in insulin sensitivity that could provide an underlying mechanism for the daily rhythm in systemic insulin sensitivity.-Carrasco-Benso, M. P., Rivero-Gutierrez, B., Lopez-Minguez, J., Anzola, A., Diez-Noguera, A., Madrid, J. A., Lujan, J. A., Martínez-Augustin, O., Scheer, F. A. J. L., Garaulet, M. Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity. SN - 1530-6860 UR - https://www.unboundmedicine.com/medline/citation/27256623/Human_adipose_tissue_expresses_intrinsic_circadian_rhythm_in_insulin_sensitivity_ L2 - http://www.fasebj.org/doi/full/10.1096/fj.201600269RR?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -