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Multilaboratory evaluation of 15 bioassays for (eco)toxicity screening and hazard ranking of engineered nanomaterials: FP7 project NANOVALID.
Nanotoxicology. 2016 11; 10(9):1229-42.N

Abstract

Within EU FP7 project NANOVALID, the (eco)toxicity of 7 well-characterized engineered nanomaterials (NMs) was evaluated by 15 bioassays in 4 laboratories. The highest tested nominal concentration of NMs was 100 mg/l. The panel of the bioassays yielded the following toxicity order: Ag > ZnO > CuO > TiO2 > MWCNTs > SiO2 > Au. Ag, ZnO and CuO proved very toxic in the majority of assays, assumingly due to dissolution. The latter was supported by the parallel analysis of the toxicity of respective soluble metal salts. The most sensitive tests/species were Daphnia magna (towards Ag NMs, 24-h EC50 = 0.003 mg Ag/l), algae Raphidocelis subcapitata (ZnO and CuO, 72-h EC50 = 0.14 mg Zn/l and 0.7 mg Cu/l, respectively) and murine fibroblasts BALB/3T3 (CuO, 48-h EC50 = 0.7 mg Cu/l). MWCNTs showed toxicity only towards rat alveolar macrophages (EC50 = 15.3 mg/l) assumingly due to high aspect ratio and TiO2 towards R. subcapitata (EC50 = 6.8 mg Ti/l) due to agglomeration of TiO2 and entrapment of algal cells. Finally, we constructed a decision tree to select the bioassays for hazard ranking of NMs. For NM testing, we recommend a multitrophic suite of 4 in vitro (eco)toxicity assays: 48-h D. magna immobilization (OECD202), 72-h R. subcapitata growth inhibition (OECD201), 30-min Vibrio fischeri bioluminescence inhibition (ISO2010) and 48-h murine fibroblast BALB/3T3 neutral red uptake in vitro (OECD129) representing crustaceans, algae, bacteria and mammalian cells, respectively. Notably, our results showed that these assays, standardized for toxicity evaluation of "regular" chemicals, proved efficient also for shortlisting of hazardous NMs. Additional assays are recommended for immunotoxicity evaluation of high aspect ratio NMs (such as MWCNTs).

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Authors+Show Affiliations

Bondarenko OM
a Laboratory of Environmental Toxicology , National Institute of Chemical Physics and Biophysics , Tallinn , Estonia .
Heinlaan M
a Laboratory of Environmental Toxicology , National Institute of Chemical Physics and Biophysics , Tallinn , Estonia .
Sihtmäe M
a Laboratory of Environmental Toxicology , National Institute of Chemical Physics and Biophysics , Tallinn , Estonia .
Ivask A
a Laboratory of Environmental Toxicology , National Institute of Chemical Physics and Biophysics , Tallinn , Estonia .
Kurvet I
a Laboratory of Environmental Toxicology , National Institute of Chemical Physics and Biophysics , Tallinn , Estonia .
Joonas E
a Laboratory of Environmental Toxicology , National Institute of Chemical Physics and Biophysics , Tallinn , Estonia . b Faculty of Science and Technology , Institute of Ecology and Earth Sciences, Tartu University , Tartu , Estonia .
Jemec A
c Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
Mannerström M
d The Finnish Centre for Alternative Methods (FICAM) , School of Medicine, University of Tampere , Tampere , Finland .
Heinonen T
d The Finnish Centre for Alternative Methods (FICAM) , School of Medicine, University of Tampere , Tampere , Finland .
Rekulapelly R
e The Centre for Cellular & Molecular Biology, Habsiguda , Hyderabad, Telangana , India , and.
Singh S
e The Centre for Cellular & Molecular Biology, Habsiguda , Hyderabad, Telangana , India , and.
Zou J
f Hearing and Balance Research Unit , Field of Oto-Laryngology, School of Medicine, University of Tampere , Tampere , Finland.
Pyykkö I
f Hearing and Balance Research Unit , Field of Oto-Laryngology, School of Medicine, University of Tampere , Tampere , Finland.
Drobne D
c Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
Kahru A
a Laboratory of Environmental Toxicology , National Institute of Chemical Physics and Biophysics , Tallinn , Estonia .

MeSH

Aliivibrio fischeriAnimalsBiological AssayCells, CulturedChlorophytaDaphniaEcotoxicologyFibroblastsHazardous SubstancesLaboratoriesNanostructuresRatsRisk AssessmentSolubilitySurface PropertiesToxicity Tests

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27259032

Citation

Bondarenko, Olesja M., et al. "Multilaboratory Evaluation of 15 Bioassays for (eco)toxicity Screening and Hazard Ranking of Engineered Nanomaterials: FP7 Project NANOVALID." Nanotoxicology, vol. 10, no. 9, 2016, pp. 1229-42.
Bondarenko OM, Heinlaan M, Sihtmäe M, et al. Multilaboratory evaluation of 15 bioassays for (eco)toxicity screening and hazard ranking of engineered nanomaterials: FP7 project NANOVALID. Nanotoxicology. 2016;10(9):1229-42.
Bondarenko, O. M., Heinlaan, M., Sihtmäe, M., Ivask, A., Kurvet, I., Joonas, E., Jemec, A., Mannerström, M., Heinonen, T., Rekulapelly, R., Singh, S., Zou, J., Pyykkö, I., Drobne, D., & Kahru, A. (2016). Multilaboratory evaluation of 15 bioassays for (eco)toxicity screening and hazard ranking of engineered nanomaterials: FP7 project NANOVALID. Nanotoxicology, 10(9), 1229-42. https://doi.org/10.1080/17435390.2016.1196251
Bondarenko OM, et al. Multilaboratory Evaluation of 15 Bioassays for (eco)toxicity Screening and Hazard Ranking of Engineered Nanomaterials: FP7 Project NANOVALID. Nanotoxicology. 2016;10(9):1229-42. PubMed PMID: 27259032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multilaboratory evaluation of 15 bioassays for (eco)toxicity screening and hazard ranking of engineered nanomaterials: FP7 project NANOVALID. AU - Bondarenko,Olesja M, AU - Heinlaan,Margit, AU - Sihtmäe,Mariliis, AU - Ivask,Angela, AU - Kurvet,Imbi, AU - Joonas,Elise, AU - Jemec,Anita, AU - Mannerström,Marika, AU - Heinonen,Tuula, AU - Rekulapelly,Rohit, AU - Singh,Shashi, AU - Zou,Jing, AU - Pyykkö,Ilmari, AU - Drobne,Damjana, AU - Kahru,Anne, Y1 - 2016/06/28/ PY - 2016/6/4/entrez PY - 2016/6/4/pubmed PY - 2017/6/16/medline KW - Metals KW - multitrophic test battery KW - physico-chemical characterization KW - risk assessment KW - solubilization SP - 1229 EP - 42 JF - Nanotoxicology JO - Nanotoxicology VL - 10 IS - 9 N2 - Within EU FP7 project NANOVALID, the (eco)toxicity of 7 well-characterized engineered nanomaterials (NMs) was evaluated by 15 bioassays in 4 laboratories. The highest tested nominal concentration of NMs was 100 mg/l. The panel of the bioassays yielded the following toxicity order: Ag > ZnO > CuO > TiO2 > MWCNTs > SiO2 > Au. Ag, ZnO and CuO proved very toxic in the majority of assays, assumingly due to dissolution. The latter was supported by the parallel analysis of the toxicity of respective soluble metal salts. The most sensitive tests/species were Daphnia magna (towards Ag NMs, 24-h EC50 = 0.003 mg Ag/l), algae Raphidocelis subcapitata (ZnO and CuO, 72-h EC50 = 0.14 mg Zn/l and 0.7 mg Cu/l, respectively) and murine fibroblasts BALB/3T3 (CuO, 48-h EC50 = 0.7 mg Cu/l). MWCNTs showed toxicity only towards rat alveolar macrophages (EC50 = 15.3 mg/l) assumingly due to high aspect ratio and TiO2 towards R. subcapitata (EC50 = 6.8 mg Ti/l) due to agglomeration of TiO2 and entrapment of algal cells. Finally, we constructed a decision tree to select the bioassays for hazard ranking of NMs. For NM testing, we recommend a multitrophic suite of 4 in vitro (eco)toxicity assays: 48-h D. magna immobilization (OECD202), 72-h R. subcapitata growth inhibition (OECD201), 30-min Vibrio fischeri bioluminescence inhibition (ISO2010) and 48-h murine fibroblast BALB/3T3 neutral red uptake in vitro (OECD129) representing crustaceans, algae, bacteria and mammalian cells, respectively. Notably, our results showed that these assays, standardized for toxicity evaluation of "regular" chemicals, proved efficient also for shortlisting of hazardous NMs. Additional assays are recommended for immunotoxicity evaluation of high aspect ratio NMs (such as MWCNTs). SN - 1743-5404 UR - https://www.unboundmedicine.com/medline/citation/27259032/Multilaboratory_evaluation_of_15_bioassays_for__eco_toxicity_screening_and_hazard_ranking_of_engineered_nanomaterials:_FP7_project_NANOVALID_ DB - PRIME DP - Unbound Medicine ER -