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Molecular characterization and confocal laser scanning microscopic study of Pygidiopsis macrostomum (Trematoda: Heterophyidae) parasites of guppies Poecilia vivipara.
. 2017 Feb; 40(2):191-203.

Abstract

Pygidiopsis macrostomum and Ascocotyle (Phagicola) pindoramensis (Digenea: Heterophyidae) parasitize guppies as intermediate hosts and, respectively, fish-eating mammals or birds as definitive hosts. Heterophyids have zoonotic potential, and molecular studies associated with morphological and ecological aspects have helped to clarify their taxonomy and phylogeny. Poecilia vivipara naturally parasitized by metacercariae of both species (100% prevalence) exhibit no external signs of parasitism. In this work, four new sequences of P. macrostomum (18S rDNA, 28S rDNA and ITS2 rDNA) and one new sequence of A. (P.) pindoramensis (mtDNA cox-1) are presented. Phylogeny reconstructions linked P. macrostomum to other heterophyids, but the separation of the Heterophyidae and Opisthorchiidae remains unclear. Additionally, we used indirect immunocytochemistry and the phalloidin-fluorescence techniques allied with confocal laser scanning microscopy to describe muscular and neuronal structures of P. macrostomum. A complex arrangement of muscular fibres is associated with the tegument, suckers, gut and reproductive system. Radial fibres around the ventral sucker are thick, branched and extend to the body wall. High-resolution confocal imaging revealed a typical digenean muscular arrangement and important heterophyid morphological traits. These data will support future control measures to reduce the parasitism in guppies reared in fish farming systems, especially for aquarium and experimental purposes.

Authors+Show Affiliations

Laboratório de Avaliação e Promoção da Saúde Ambiental (LAPSA), Fiocruz, Rio de Janeiro, Brazil.Laboratório de Avaliação e Promoção da Saúde Ambiental (LAPSA), Fiocruz, Rio de Janeiro, Brazil.Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Brazil.Núcleo de Análises de Biomoléculas do Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Viçosa, Brazil.Laboratório de Avaliação e Promoção da Saúde Ambiental (LAPSA), Fiocruz, Rio de Janeiro, Brazil.Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Brazil.Laboratório de Avaliação e Promoção da Saúde Ambiental (LAPSA), Fiocruz, Rio de Janeiro, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27260384

Citation

Borges, J N., et al. "Molecular Characterization and Confocal Laser Scanning Microscopic Study of Pygidiopsis Macrostomum (Trematoda: Heterophyidae) Parasites of Guppies Poecilia Vivipara." Journal of Fish Diseases, vol. 40, no. 2, 2017, pp. 191-203.
Borges JN, Costa VS, Mantovani C, et al. Molecular characterization and confocal laser scanning microscopic study of Pygidiopsis macrostomum (Trematoda: Heterophyidae) parasites of guppies Poecilia vivipara. J Fish Dis. 2017;40(2):191-203.
Borges, J. N., Costa, V. S., Mantovani, C., Barros, E., Santos, E. G., Mafra, C. L., & Santos, C. P. (2017). Molecular characterization and confocal laser scanning microscopic study of Pygidiopsis macrostomum (Trematoda: Heterophyidae) parasites of guppies Poecilia vivipara. Journal of Fish Diseases, 40(2), 191-203. https://doi.org/10.1111/jfd.12504
Borges JN, et al. Molecular Characterization and Confocal Laser Scanning Microscopic Study of Pygidiopsis Macrostomum (Trematoda: Heterophyidae) Parasites of Guppies Poecilia Vivipara. J Fish Dis. 2017;40(2):191-203. PubMed PMID: 27260384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization and confocal laser scanning microscopic study of Pygidiopsis macrostomum (Trematoda: Heterophyidae) parasites of guppies Poecilia vivipara. AU - Borges,J N, AU - Costa,V S, AU - Mantovani,C, AU - Barros,E, AU - Santos,E G N, AU - Mafra,C L, AU - Santos,C P, Y1 - 2016/06/03/ PY - 2016/01/15/received PY - 2016/04/13/revised PY - 2016/04/15/accepted PY - 2016/6/5/pubmed PY - 2017/5/20/medline PY - 2016/6/5/entrez KW - DNA KW - Ascocotyle (Phagicola) pindoramensis KW - Brazil KW - Opisthorchioidea KW - confocal laser scanning microscopy KW - fish SP - 191 EP - 203 JF - Journal of fish diseases JO - J. Fish Dis. VL - 40 IS - 2 N2 - Pygidiopsis macrostomum and Ascocotyle (Phagicola) pindoramensis (Digenea: Heterophyidae) parasitize guppies as intermediate hosts and, respectively, fish-eating mammals or birds as definitive hosts. Heterophyids have zoonotic potential, and molecular studies associated with morphological and ecological aspects have helped to clarify their taxonomy and phylogeny. Poecilia vivipara naturally parasitized by metacercariae of both species (100% prevalence) exhibit no external signs of parasitism. In this work, four new sequences of P. macrostomum (18S rDNA, 28S rDNA and ITS2 rDNA) and one new sequence of A. (P.) pindoramensis (mtDNA cox-1) are presented. Phylogeny reconstructions linked P. macrostomum to other heterophyids, but the separation of the Heterophyidae and Opisthorchiidae remains unclear. Additionally, we used indirect immunocytochemistry and the phalloidin-fluorescence techniques allied with confocal laser scanning microscopy to describe muscular and neuronal structures of P. macrostomum. A complex arrangement of muscular fibres is associated with the tegument, suckers, gut and reproductive system. Radial fibres around the ventral sucker are thick, branched and extend to the body wall. High-resolution confocal imaging revealed a typical digenean muscular arrangement and important heterophyid morphological traits. These data will support future control measures to reduce the parasitism in guppies reared in fish farming systems, especially for aquarium and experimental purposes. SN - 1365-2761 UR - https://www.unboundmedicine.com/medline/citation/27260384/Molecular_characterization_and_confocal_laser_scanning_microscopic_study_of_Pygidiopsis_macrostomum__Trematoda:_Heterophyidae__parasites_of_guppies_Poecilia_vivipara_ L2 - https://doi.org/10.1111/jfd.12504 DB - PRIME DP - Unbound Medicine ER -