Tags

Type your tag names separated by a space and hit enter

Profiling of the toxicity mechanisms of coated and uncoated silver nanoparticles to yeast Saccharomyces cerevisiae BY4741 using a set of its 9 single-gene deletion mutants defective in oxidative stress response, cell wall or membrane integrity and endocytosis.
Toxicol In Vitro. 2016 Sep; 35:149-62.TV

Abstract

The widespread use of nanosilver in various antibacterial, antifungal, and antiviral products warrants the studies of the toxicity pathways of nanosilver-enabled materials toward microbes and viruses. We profiled the toxicity mechanisms of uncoated, casein-coated, and polyvinylpyrrolidone-coated silver nanoparticles (AgNPs) using Saccharomyces cerevisiae wild-type (wt) and its 9 single-gene deletion mutants defective in oxidative stress (OS) defense, cell wall/membrane integrity, and endocytosis. The 48-h growth inhibition assay in organic-rich growth medium and 24-h cell viability assay in deionized (DI) water were applied whereas AgNO3, H2O2, and SDS served as positive controls. Both coated AgNPs (primary size 8-12nm) were significantly more toxic than the uncoated (~85nm) AgNPs. All studied AgNPs were ~30 times more toxic if exposed to yeast cells in DI water than in the rich growth medium: the IC50 based on nominal concentration of AgNPs in the growth inhibition test ranged from 77 to 576mg Ag/L and in the cell viability test from 2.7 to 18.7mg Ag/L, respectively. Confocal microscopy showed that wt but not endocytosis mutant (end3Δ) internalized AgNPs. Comparison of toxicity patterns of wt and mutant strains defective in OS defense and membrane integrity revealed that the toxicity of the studied AgNPs to S. cerevisiae was not caused by the OS or cell wall/membrane permeabilization.

Authors+Show Affiliations

Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn 12618, Estonia; Faculty of Chemical and Materials Technology, Tallinn University of Technology, Ehitajate tee 5, Tallinn 19086, Estonia. Electronic address: sandra.kaosaar@kbfi.ee.Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn 12618, Estonia. Electronic address: anne.kahru@kbfi.ee.Department of Earth and Environmental Sciences, Research Centre POLARIS, University of Milano-Bicocca, 1 Piazza della Scienza, Milano 20126, Italy. Electronic address: paride.mantecca@unimib.it.Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn 12618, Estonia. Electronic address: kaja.kasemets@kbfi.ee.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27260961

Citation

Käosaar, Sandra, et al. "Profiling of the Toxicity Mechanisms of Coated and Uncoated Silver Nanoparticles to Yeast Saccharomyces Cerevisiae BY4741 Using a Set of Its 9 Single-gene Deletion Mutants Defective in Oxidative Stress Response, Cell Wall or Membrane Integrity and Endocytosis." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 35, 2016, pp. 149-62.
Käosaar S, Kahru A, Mantecca P, et al. Profiling of the toxicity mechanisms of coated and uncoated silver nanoparticles to yeast Saccharomyces cerevisiae BY4741 using a set of its 9 single-gene deletion mutants defective in oxidative stress response, cell wall or membrane integrity and endocytosis. Toxicol In Vitro. 2016;35:149-62.
Käosaar, S., Kahru, A., Mantecca, P., & Kasemets, K. (2016). Profiling of the toxicity mechanisms of coated and uncoated silver nanoparticles to yeast Saccharomyces cerevisiae BY4741 using a set of its 9 single-gene deletion mutants defective in oxidative stress response, cell wall or membrane integrity and endocytosis. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 35, 149-62. https://doi.org/10.1016/j.tiv.2016.05.018
Käosaar S, et al. Profiling of the Toxicity Mechanisms of Coated and Uncoated Silver Nanoparticles to Yeast Saccharomyces Cerevisiae BY4741 Using a Set of Its 9 Single-gene Deletion Mutants Defective in Oxidative Stress Response, Cell Wall or Membrane Integrity and Endocytosis. Toxicol In Vitro. 2016;35:149-62. PubMed PMID: 27260961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profiling of the toxicity mechanisms of coated and uncoated silver nanoparticles to yeast Saccharomyces cerevisiae BY4741 using a set of its 9 single-gene deletion mutants defective in oxidative stress response, cell wall or membrane integrity and endocytosis. AU - Käosaar,Sandra, AU - Kahru,Anne, AU - Mantecca,Paride, AU - Kasemets,Kaja, Y1 - 2016/05/31/ PY - 2015/11/19/received PY - 2016/05/20/revised PY - 2016/05/28/accepted PY - 2016/6/5/entrez PY - 2016/6/5/pubmed PY - 2017/4/5/medline KW - Dissolution KW - EUROSCARF strains KW - Endocytosis KW - Silver nanoparticles KW - Speciation SP - 149 EP - 62 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 35 N2 - The widespread use of nanosilver in various antibacterial, antifungal, and antiviral products warrants the studies of the toxicity pathways of nanosilver-enabled materials toward microbes and viruses. We profiled the toxicity mechanisms of uncoated, casein-coated, and polyvinylpyrrolidone-coated silver nanoparticles (AgNPs) using Saccharomyces cerevisiae wild-type (wt) and its 9 single-gene deletion mutants defective in oxidative stress (OS) defense, cell wall/membrane integrity, and endocytosis. The 48-h growth inhibition assay in organic-rich growth medium and 24-h cell viability assay in deionized (DI) water were applied whereas AgNO3, H2O2, and SDS served as positive controls. Both coated AgNPs (primary size 8-12nm) were significantly more toxic than the uncoated (~85nm) AgNPs. All studied AgNPs were ~30 times more toxic if exposed to yeast cells in DI water than in the rich growth medium: the IC50 based on nominal concentration of AgNPs in the growth inhibition test ranged from 77 to 576mg Ag/L and in the cell viability test from 2.7 to 18.7mg Ag/L, respectively. Confocal microscopy showed that wt but not endocytosis mutant (end3Δ) internalized AgNPs. Comparison of toxicity patterns of wt and mutant strains defective in OS defense and membrane integrity revealed that the toxicity of the studied AgNPs to S. cerevisiae was not caused by the OS or cell wall/membrane permeabilization. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/27260961/Profiling_of_the_toxicity_mechanisms_of_coated_and_uncoated_silver_nanoparticles_to_yeast_Saccharomyces_cerevisiae_BY4741_using_a_set_of_its_9_single_gene_deletion_mutants_defective_in_oxidative_stress_response_cell_wall_or_membrane_integrity_and_endocytosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(16)30113-8 DB - PRIME DP - Unbound Medicine ER -