Tags

Type your tag names separated by a space and hit enter

Molecular basis of retinol anti-ageing properties in naturally aged human skin in vivo.
Int J Cosmet Sci. 2017 Feb; 39(1):56-65.IJ

Abstract

OBJECTIVE

Retinoic acid has been shown to improve the aged-appearing skin. However, less is known about the anti-ageing effects of retinol (ROL, vitamin A), a precursor of retinoic acid, in aged human skin in vivo. This study aimed to investigate the molecular basis of ROL anti-ageing properties in naturally aged human skin in vivo.

METHODS

Sun-protected buttock skin (76 ± 6 years old, n = 12) was topically treated with 0.4% ROL and its vehicle for 7 days. The effects of topical ROL on skin epidermis and dermis were evaluated by immunohistochemistry, in situ hybridization, Northern analysis, real-time RT-PCR and Western analysis. Collagen fibrils nanoscale structure and surface topology were analysed by atomic force microscopy.

RESULTS

Topical ROL shows remarkable anti-ageing effects through three major types of skin cells: epidermal keratinocytes, dermal endothelial cells and fibroblasts. Topical ROL significantly increased epidermal thickness by stimulating keratinocytes proliferation and upregulation of c-Jun transcription factor. In addition to epidermal changes, topical ROL significantly improved dermal extracellular matrix (ECM) microenvironment; increasing dermal vascularity by stimulating endothelial cells proliferation and ECM production (type I collagen, fibronectin and elastin) by activating dermal fibroblasts. Topical ROL also stimulates TGF-β/CTGF pathway, the major regulator of ECM homeostasis, and thus enriched the deposition of ECM in aged human skin in vivo. 0.4% topical ROL achieved similar results as seen with topical retinoic acid, the biologically active form of ROL, without causing noticeable signs of retinoid side effects.

CONCLUSION

0.4% topical ROL shows remarkable anti-ageing effects through improvement of the homeostasis of epidermis and dermis by stimulating the proliferation of keratinocytes and endothelial cells, and activating dermal fibroblasts. These data provide evidence that 0.4% topical ROL is a promising and safe treatment to improve the naturally aged human skin.

Authors+Show Affiliations

Department of Dermatology, University of Michigan Medical School, Ann Arbor, 48109, Michigan, MI, U.S.A.Department of Dermatology, University of Michigan Medical School, Ann Arbor, 48109, Michigan, MI, U.S.A.Department of Dermatology, University of Michigan Medical School, Ann Arbor, 48109, Michigan, MI, U.S.A.Department of Dermatology, University of Michigan Medical School, Ann Arbor, 48109, Michigan, MI, U.S.A.Department of Dermatology, University of Michigan Medical School, Ann Arbor, 48109, Michigan, MI, U.S.A.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27261203

Citation

Shao, Y, et al. "Molecular Basis of Retinol Anti-ageing Properties in Naturally Aged Human Skin in Vivo." International Journal of Cosmetic Science, vol. 39, no. 1, 2017, pp. 56-65.
Shao Y, He T, Fisher GJ, et al. Molecular basis of retinol anti-ageing properties in naturally aged human skin in vivo. Int J Cosmet Sci. 2017;39(1):56-65.
Shao, Y., He, T., Fisher, G. J., Voorhees, J. J., & Quan, T. (2017). Molecular basis of retinol anti-ageing properties in naturally aged human skin in vivo. International Journal of Cosmetic Science, 39(1), 56-65. https://doi.org/10.1111/ics.12348
Shao Y, et al. Molecular Basis of Retinol Anti-ageing Properties in Naturally Aged Human Skin in Vivo. Int J Cosmet Sci. 2017;39(1):56-65. PubMed PMID: 27261203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular basis of retinol anti-ageing properties in naturally aged human skin in vivo. AU - Shao,Y, AU - He,T, AU - Fisher,G J, AU - Voorhees,J J, AU - Quan,T, Y1 - 2016/07/04/ PY - 2016/02/22/received PY - 2016/05/25/accepted PY - 2016/6/5/pubmed PY - 2017/8/16/medline PY - 2016/6/5/entrez KW - TGF-β KW - extracellular matrix KW - retinoid KW - skin ageing KW - vascularity SP - 56 EP - 65 JF - International journal of cosmetic science JO - Int J Cosmet Sci VL - 39 IS - 1 N2 - OBJECTIVE: Retinoic acid has been shown to improve the aged-appearing skin. However, less is known about the anti-ageing effects of retinol (ROL, vitamin A), a precursor of retinoic acid, in aged human skin in vivo. This study aimed to investigate the molecular basis of ROL anti-ageing properties in naturally aged human skin in vivo. METHODS: Sun-protected buttock skin (76 ± 6 years old, n = 12) was topically treated with 0.4% ROL and its vehicle for 7 days. The effects of topical ROL on skin epidermis and dermis were evaluated by immunohistochemistry, in situ hybridization, Northern analysis, real-time RT-PCR and Western analysis. Collagen fibrils nanoscale structure and surface topology were analysed by atomic force microscopy. RESULTS: Topical ROL shows remarkable anti-ageing effects through three major types of skin cells: epidermal keratinocytes, dermal endothelial cells and fibroblasts. Topical ROL significantly increased epidermal thickness by stimulating keratinocytes proliferation and upregulation of c-Jun transcription factor. In addition to epidermal changes, topical ROL significantly improved dermal extracellular matrix (ECM) microenvironment; increasing dermal vascularity by stimulating endothelial cells proliferation and ECM production (type I collagen, fibronectin and elastin) by activating dermal fibroblasts. Topical ROL also stimulates TGF-β/CTGF pathway, the major regulator of ECM homeostasis, and thus enriched the deposition of ECM in aged human skin in vivo. 0.4% topical ROL achieved similar results as seen with topical retinoic acid, the biologically active form of ROL, without causing noticeable signs of retinoid side effects. CONCLUSION: 0.4% topical ROL shows remarkable anti-ageing effects through improvement of the homeostasis of epidermis and dermis by stimulating the proliferation of keratinocytes and endothelial cells, and activating dermal fibroblasts. These data provide evidence that 0.4% topical ROL is a promising and safe treatment to improve the naturally aged human skin. SN - 1468-2494 UR - https://www.unboundmedicine.com/medline/citation/27261203/Molecular_basis_of_retinol_anti_ageing_properties_in_naturally_aged_human_skin_in_vivo_ L2 - https://doi.org/10.1111/ics.12348 DB - PRIME DP - Unbound Medicine ER -