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The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy.
Allergy. 2017 Feb; 72(2):266-273.A

Abstract

BACKGROUND

IgG to galactose-α-1,3-galactose (α-gal) are highly abundant natural antibodies (Ab) in humans. α-Gal-specific IgE Ab cause a special form of meat allergy characterized by severe systemic reactions 3-7 h after consumption of red meat. We investigated 20 patients who experienced such reactions and characterized their α-gal-specific IgE and IgG responses in more detail.

METHODS

α-Gal-specific IgE was determined by ImmunoCAP. IgE reactivity to meat extract and bovine gamma globulin (BGG) was assessed by immunoblotting and ELISA, respectively. In some experiments, sera were pre-incubated with α-gal or protein G to deplete IgG Ab. α-Gal-specific IgG1-4 Ab in individuals with and without meat allergy were assessed by ELISA.

RESULTS

In immunoblots, BGG was the most frequently recognized meat protein. Binding of IgE and IgG to BGG was confirmed by ELISA and completely abolished after pre-incubation with α-gal. Neither the depletion of autologous α-gal-specific IgG Ab nor the addition of α-gal-specific IgG Ab from nonallergic individuals changed the IgE recognition of BGG of meat-allergic patients. Meat-allergic patients showed significantly higher α-gal-specific IgG1 and IgG3 Ab than nonallergic individuals, whereas the latter showed significantly higher levels of α-gal-specific IgG4 Ab.

CONCLUSION

Patients with delayed meat allergy display IgE and IgG Ab that selectively recognize the α-gal epitope on BGG. Their enhanced α-gal-specific IgE levels are accompanied by high levels of α-gal-specific IgG1 devoid of IgE-blocking activity. This subclass distribution is atypical for food allergies and distinct from natural α-gal IgG responses in nonallergic individuals.

Authors+Show Affiliations

Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.Allergy Clinic Reumannplatz, Vienna, Austria.Allergy Clinic Rennweg, Vienna, Austria.Allergy Clinic Floridsdorf, Vienna, Austria.Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.Department of Internal Medicine, Medical University of Vienna, Vienna, Austria.Department of Thoracic Surgery, Medical University of Vienna, Vienna, Austria.Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27261450

Citation

Kollmann, D, et al. "The Quantity and Quality of Α-gal-specific Antibodies Differ in Individuals With and Without Delayed Red Meat Allergy." Allergy, vol. 72, no. 2, 2017, pp. 266-273.
Kollmann D, Nagl B, Ebner C, et al. The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy. Allergy. 2017;72(2):266-273.
Kollmann, D., Nagl, B., Ebner, C., Emminger, W., Wöhrl, S., Kitzmüller, C., Vrtala, S., Mangold, A., Ankersmit, H. J., & Bohle, B. (2017). The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy. Allergy, 72(2), 266-273. https://doi.org/10.1111/all.12948
Kollmann D, et al. The Quantity and Quality of Α-gal-specific Antibodies Differ in Individuals With and Without Delayed Red Meat Allergy. Allergy. 2017;72(2):266-273. PubMed PMID: 27261450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy. AU - Kollmann,D, AU - Nagl,B, AU - Ebner,C, AU - Emminger,W, AU - Wöhrl,S, AU - Kitzmüller,C, AU - Vrtala,S, AU - Mangold,A, AU - Ankersmit,H-J, AU - Bohle,B, Y1 - 2016/07/15/ PY - 2016/05/31/accepted PY - 2016/6/5/pubmed PY - 2017/12/8/medline PY - 2016/6/5/entrez KW - IgE KW - allergens and epitopes KW - food allergy SP - 266 EP - 273 JF - Allergy JO - Allergy VL - 72 IS - 2 N2 - BACKGROUND: IgG to galactose-α-1,3-galactose (α-gal) are highly abundant natural antibodies (Ab) in humans. α-Gal-specific IgE Ab cause a special form of meat allergy characterized by severe systemic reactions 3-7 h after consumption of red meat. We investigated 20 patients who experienced such reactions and characterized their α-gal-specific IgE and IgG responses in more detail. METHODS: α-Gal-specific IgE was determined by ImmunoCAP. IgE reactivity to meat extract and bovine gamma globulin (BGG) was assessed by immunoblotting and ELISA, respectively. In some experiments, sera were pre-incubated with α-gal or protein G to deplete IgG Ab. α-Gal-specific IgG1-4 Ab in individuals with and without meat allergy were assessed by ELISA. RESULTS: In immunoblots, BGG was the most frequently recognized meat protein. Binding of IgE and IgG to BGG was confirmed by ELISA and completely abolished after pre-incubation with α-gal. Neither the depletion of autologous α-gal-specific IgG Ab nor the addition of α-gal-specific IgG Ab from nonallergic individuals changed the IgE recognition of BGG of meat-allergic patients. Meat-allergic patients showed significantly higher α-gal-specific IgG1 and IgG3 Ab than nonallergic individuals, whereas the latter showed significantly higher levels of α-gal-specific IgG4 Ab. CONCLUSION: Patients with delayed meat allergy display IgE and IgG Ab that selectively recognize the α-gal epitope on BGG. Their enhanced α-gal-specific IgE levels are accompanied by high levels of α-gal-specific IgG1 devoid of IgE-blocking activity. This subclass distribution is atypical for food allergies and distinct from natural α-gal IgG responses in nonallergic individuals. SN - 1398-9995 UR - https://www.unboundmedicine.com/medline/citation/27261450/The_quantity_and_quality_of_α_gal_specific_antibodies_differ_in_individuals_with_and_without_delayed_red_meat_allergy_ L2 - https://doi.org/10.1111/all.12948 DB - PRIME DP - Unbound Medicine ER -