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Simultaneous quantification of vortioxetine, carvedilol and its active metabolite 4-hydroxyphenyl carvedilol in rat plasma by UPLC-MS/MS: Application to their pharmacokinetic interaction study.
J Pharm Biomed Anal. 2016 Sep 05; 128:184-190.JP

Abstract

To establish a rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the determination of vortioxetine, carvedilol and its metabolite 4-hydroxyphenyl carvedilol in rat plasma. The analytes and the internal standard (diazepam) were separated on an Acquity UPLC BEH C18 chromatography column (2.1mm×50mm, 1.7μm) using gradient elution with a mobile phase of acetonitrile and 0.1% formic acid in water at a flow rate of 0.4mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 299.2→150.1 for vortioxetine, m/z 407.2→100.3 for carvedilol, m/z 423.2→100.1 for 4-hydroxyphenyl carvedilol and m/z 285.2→193.1 for diazepam (IS) using a positive electrospray ionization interface. The method was validated over a concentration range of 0.5-100ng/mL for vortioxetine, 0.5-1000ng/mL for carvedilol and 0.1-50ng/mL for 4-hydroxyphenyl carvedilol. Total time for each chromatograph was 3.0min. The intra- and inter-day precision and accuracy of the quality control samples at low, medium, and high concentration levels exhibited relative standard deviations (RSD)<11.6% and the accuracy values ranged from -12.2% to 11.3%. The analytical method was successfully applied to a pharmacokinetic interaction study of vortioxetine and carvedilol after oral administration vortioxetine and carvedilol in rats. Results suggested that the co-administration of vortioxetine and carvedilol results in a significant drug interaction in rats.

Authors+Show Affiliations

Wenzhou People's Hospital, Wenzhou 325000, China.The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.Medical College of Henan University of Science and Technology, Luoyang 471003, China.Medical College of Henan University of Science and Technology, Luoyang 471003, China.The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: xuzhisheng1111@163.com.The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address: wzshaomengmeng@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27262994

Citation

Huang, Yi, et al. "Simultaneous Quantification of Vortioxetine, Carvedilol and Its Active Metabolite 4-hydroxyphenyl Carvedilol in Rat Plasma By UPLC-MS/MS: Application to Their Pharmacokinetic Interaction Study." Journal of Pharmaceutical and Biomedical Analysis, vol. 128, 2016, pp. 184-190.
Huang Y, Zheng S, Pan Y, et al. Simultaneous quantification of vortioxetine, carvedilol and its active metabolite 4-hydroxyphenyl carvedilol in rat plasma by UPLC-MS/MS: Application to their pharmacokinetic interaction study. J Pharm Biomed Anal. 2016;128:184-190.
Huang, Y., Zheng, S., Pan, Y., Li, T., Xu, Z. S., & Shao, M. M. (2016). Simultaneous quantification of vortioxetine, carvedilol and its active metabolite 4-hydroxyphenyl carvedilol in rat plasma by UPLC-MS/MS: Application to their pharmacokinetic interaction study. Journal of Pharmaceutical and Biomedical Analysis, 128, 184-190. https://doi.org/10.1016/j.jpba.2016.05.029
Huang Y, et al. Simultaneous Quantification of Vortioxetine, Carvedilol and Its Active Metabolite 4-hydroxyphenyl Carvedilol in Rat Plasma By UPLC-MS/MS: Application to Their Pharmacokinetic Interaction Study. J Pharm Biomed Anal. 2016 Sep 5;128:184-190. PubMed PMID: 27262994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of vortioxetine, carvedilol and its active metabolite 4-hydroxyphenyl carvedilol in rat plasma by UPLC-MS/MS: Application to their pharmacokinetic interaction study. AU - Huang,Yi, AU - Zheng,Shuangli, AU - Pan,Yongyang, AU - Li,Tao, AU - Xu,Zhi-Sheng, AU - Shao,Meng-Meng, Y1 - 2016/05/19/ PY - 2016/02/20/received PY - 2016/05/17/revised PY - 2016/05/18/accepted PY - 2016/6/6/entrez PY - 2016/6/6/pubmed PY - 2017/5/10/medline KW - 4-hydroxyphenyl carvedilol KW - Carvedilol KW - Pharmacokinetics KW - UPLC–MS/MS KW - Vortioxetine SP - 184 EP - 190 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 128 N2 - To establish a rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the determination of vortioxetine, carvedilol and its metabolite 4-hydroxyphenyl carvedilol in rat plasma. The analytes and the internal standard (diazepam) were separated on an Acquity UPLC BEH C18 chromatography column (2.1mm×50mm, 1.7μm) using gradient elution with a mobile phase of acetonitrile and 0.1% formic acid in water at a flow rate of 0.4mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 299.2→150.1 for vortioxetine, m/z 407.2→100.3 for carvedilol, m/z 423.2→100.1 for 4-hydroxyphenyl carvedilol and m/z 285.2→193.1 for diazepam (IS) using a positive electrospray ionization interface. The method was validated over a concentration range of 0.5-100ng/mL for vortioxetine, 0.5-1000ng/mL for carvedilol and 0.1-50ng/mL for 4-hydroxyphenyl carvedilol. Total time for each chromatograph was 3.0min. The intra- and inter-day precision and accuracy of the quality control samples at low, medium, and high concentration levels exhibited relative standard deviations (RSD)<11.6% and the accuracy values ranged from -12.2% to 11.3%. The analytical method was successfully applied to a pharmacokinetic interaction study of vortioxetine and carvedilol after oral administration vortioxetine and carvedilol in rats. Results suggested that the co-administration of vortioxetine and carvedilol results in a significant drug interaction in rats. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/27262994/Simultaneous_quantification_of_vortioxetine_carvedilol_and_its_active_metabolite_4_hydroxyphenyl_carvedilol_in_rat_plasma_by_UPLC_MS/MS:_Application_to_their_pharmacokinetic_interaction_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(16)30268-0 DB - PRIME DP - Unbound Medicine ER -