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Comparative Transcriptome Analysis of Two Ipomoea aquatica Forsk. Cultivars Targeted To Explore Possible Mechanism of Genotype-Dependent Accumulation of Cadmium.
J Agric Food Chem. 2016 Jun 29; 64(25):5241-50.JA

Abstract

A low-shoot-Cd (QLQ) and a high-shoot-Cd cultivar (T308) of water spinach (Ipomoea aquatica Forsk.) were used to investigate molecular mechanism of the genotype difference in cadmium (Cd) accumulation. RNA-Seq under 9 and 72 h cadmium exposures (5 mg L(-1)) were undertaken to explore Cd induced genotype differences in molecular processes. In total, 253 747 540 clean reads were assembled into 57 524 unigenes. Among them, 6136 and 10 064 unigenes were differentially expressed in QLQ and T308, respectively. Cell wall biosynthesis genes, such as GAUT and laccase, and three Cd efflux genes (Nramp5, MATE9, and YSL7) had higher expression levels in QLQ, while the genes in sulfur and glutathione metabolism pathway, e.g., sulfate transporter and cysteine synthase, showed higher expression levels in T308. These findings would be useful for further understanding of the mechanisms related to genotype-dependent Cd accumulation and developing the molecular assisted screening and breeding of low-shoot-Cd cultivars for water spinach.

Authors+Show Affiliations

State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27267580

Citation

Huang, Ying-Ying, et al. "Comparative Transcriptome Analysis of Two Ipomoea Aquatica Forsk. Cultivars Targeted to Explore Possible Mechanism of Genotype-Dependent Accumulation of Cadmium." Journal of Agricultural and Food Chemistry, vol. 64, no. 25, 2016, pp. 5241-50.
Huang YY, Shen C, Chen JX, et al. Comparative Transcriptome Analysis of Two Ipomoea aquatica Forsk. Cultivars Targeted To Explore Possible Mechanism of Genotype-Dependent Accumulation of Cadmium. J Agric Food Chem. 2016;64(25):5241-50.
Huang, Y. Y., Shen, C., Chen, J. X., He, C. T., Zhou, Q., Tan, X., Yuan, J. G., & Yang, Z. Y. (2016). Comparative Transcriptome Analysis of Two Ipomoea aquatica Forsk. Cultivars Targeted To Explore Possible Mechanism of Genotype-Dependent Accumulation of Cadmium. Journal of Agricultural and Food Chemistry, 64(25), 5241-50. https://doi.org/10.1021/acs.jafc.6b01267
Huang YY, et al. Comparative Transcriptome Analysis of Two Ipomoea Aquatica Forsk. Cultivars Targeted to Explore Possible Mechanism of Genotype-Dependent Accumulation of Cadmium. J Agric Food Chem. 2016 Jun 29;64(25):5241-50. PubMed PMID: 27267580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Transcriptome Analysis of Two Ipomoea aquatica Forsk. Cultivars Targeted To Explore Possible Mechanism of Genotype-Dependent Accumulation of Cadmium. AU - Huang,Ying-Ying, AU - Shen,Chuang, AU - Chen,Jing-Xin, AU - He,Chun-Tao, AU - Zhou,Qian, AU - Tan,Xiao, AU - Yuan,Jian-Gang, AU - Yang,Zhong-Yi, Y1 - 2016/06/20/ PY - 2016/6/9/entrez PY - 2016/6/9/pubmed PY - 2017/3/31/medline KW - DEGs KW - low-shoot-Cd accumulation KW - molecular mechanisms KW - transcriptome KW - water spinach (Ipomoea aquatica Forsk.) SP - 5241 EP - 50 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 64 IS - 25 N2 - A low-shoot-Cd (QLQ) and a high-shoot-Cd cultivar (T308) of water spinach (Ipomoea aquatica Forsk.) were used to investigate molecular mechanism of the genotype difference in cadmium (Cd) accumulation. RNA-Seq under 9 and 72 h cadmium exposures (5 mg L(-1)) were undertaken to explore Cd induced genotype differences in molecular processes. In total, 253 747 540 clean reads were assembled into 57 524 unigenes. Among them, 6136 and 10 064 unigenes were differentially expressed in QLQ and T308, respectively. Cell wall biosynthesis genes, such as GAUT and laccase, and three Cd efflux genes (Nramp5, MATE9, and YSL7) had higher expression levels in QLQ, while the genes in sulfur and glutathione metabolism pathway, e.g., sulfate transporter and cysteine synthase, showed higher expression levels in T308. These findings would be useful for further understanding of the mechanisms related to genotype-dependent Cd accumulation and developing the molecular assisted screening and breeding of low-shoot-Cd cultivars for water spinach. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/27267580/Comparative_Transcriptome_Analysis_of_Two_Ipomoea_aquatica_Forsk__Cultivars_Targeted_To_Explore_Possible_Mechanism_of_Genotype_Dependent_Accumulation_of_Cadmium_ L2 - https://doi.org/10.1021/acs.jafc.6b01267 DB - PRIME DP - Unbound Medicine ER -