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Antioxidant and antimutagenic potential of Psidium guajava leaf extracts.
Drug Chem Toxicol. 2017 Apr; 40(2):146-153.DC

Abstract

Fruits, vegetables and medicinal herbs rich in phenolics antioxidants contribute toward reduced risk of age-related diseases and cancer. In this study, Psidium guajava leaf extract was fractionated in various organic solvents viz. petroleum ether, benzene, ethyl acetate, ethanl and methanol and tested for their antioxidant and antimutagenic properties. Methanolic fraction showed maximum antioxidant activity comparable to ascorbic acid and butylated hydroxyl toluene (BHT) as tested by DPPH free radical scavenging, phosphomolybdenum, FRAP (Fe3 + reducing power) and CUPRAC (cupric ions (Cu2+) reducing ability) assays. The fraction was analyzed for antimutagenic activities against sodium azide (NaN3), methylmethane sulfonate (MMS), 2-aminofluorene (2AF) and benzo(a)pyrene (BP) in Ames Salmonella tester strains. The methanol extracted fraction at 80 μg/ml concentration inhibited above 70% mutagenicity. Further, phytochemical analysis of methanol fraction that was found to be most active revealed the presence of nine major compounds by gas chromatography-mass spectrometry (GC-MS). This data suggests that guava contains high amount of phenolics responsible for broad-spectrum antimutagenic and antioxidant properties in vitro and could be potential candidates to be explored as modern phytomedicine.

Authors+Show Affiliations

a Department of Agricultural Microbiology , Aligarh Muslim University , Aligarh , UP , India and. b James Graham Brown Cancer Center, University of Louisville , Louisville , KY , USA.a Department of Agricultural Microbiology , Aligarh Muslim University , Aligarh , UP , India and.a Department of Agricultural Microbiology , Aligarh Muslim University , Aligarh , UP , India and. b James Graham Brown Cancer Center, University of Louisville , Louisville , KY , USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27268266

Citation

Zahin, Maryam, et al. "Antioxidant and Antimutagenic Potential of Psidium Guajava Leaf Extracts." Drug and Chemical Toxicology, vol. 40, no. 2, 2017, pp. 146-153.
Zahin M, Ahmad I, Aqil F. Antioxidant and antimutagenic potential of Psidium guajava leaf extracts. Drug Chem Toxicol. 2017;40(2):146-153.
Zahin, M., Ahmad, I., & Aqil, F. (2017). Antioxidant and antimutagenic potential of Psidium guajava leaf extracts. Drug and Chemical Toxicology, 40(2), 146-153. https://doi.org/10.1080/01480545.2016.1188397
Zahin M, Ahmad I, Aqil F. Antioxidant and Antimutagenic Potential of Psidium Guajava Leaf Extracts. Drug Chem Toxicol. 2017;40(2):146-153. PubMed PMID: 27268266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and antimutagenic potential of Psidium guajava leaf extracts. AU - Zahin,Maryam, AU - Ahmad,Iqbal, AU - Aqil,Farrukh, Y1 - 2016/06/07/ PY - 2016/6/9/pubmed PY - 2017/4/5/medline PY - 2016/6/9/entrez KW - Ames test KW - GC/MS analysis KW - Psidium guajava KW - antimutagenic activity KW - antioxidant activity KW - methanolic extract SP - 146 EP - 153 JF - Drug and chemical toxicology JO - Drug Chem Toxicol VL - 40 IS - 2 N2 - Fruits, vegetables and medicinal herbs rich in phenolics antioxidants contribute toward reduced risk of age-related diseases and cancer. In this study, Psidium guajava leaf extract was fractionated in various organic solvents viz. petroleum ether, benzene, ethyl acetate, ethanl and methanol and tested for their antioxidant and antimutagenic properties. Methanolic fraction showed maximum antioxidant activity comparable to ascorbic acid and butylated hydroxyl toluene (BHT) as tested by DPPH free radical scavenging, phosphomolybdenum, FRAP (Fe3 + reducing power) and CUPRAC (cupric ions (Cu2+) reducing ability) assays. The fraction was analyzed for antimutagenic activities against sodium azide (NaN3), methylmethane sulfonate (MMS), 2-aminofluorene (2AF) and benzo(a)pyrene (BP) in Ames Salmonella tester strains. The methanol extracted fraction at 80 μg/ml concentration inhibited above 70% mutagenicity. Further, phytochemical analysis of methanol fraction that was found to be most active revealed the presence of nine major compounds by gas chromatography-mass spectrometry (GC-MS). This data suggests that guava contains high amount of phenolics responsible for broad-spectrum antimutagenic and antioxidant properties in vitro and could be potential candidates to be explored as modern phytomedicine. SN - 1525-6014 UR - https://www.unboundmedicine.com/medline/citation/27268266/Antioxidant_and_antimutagenic_potential_of_Psidium_guajava_leaf_extracts_ DB - PRIME DP - Unbound Medicine ER -