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Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding of synthesized low-cost sugar mixture.
Bioresour Technol. 2016 Sep; 216:503-10.BT

Abstract

Cellulase is a prerequisite for the bioconversion of lignocellulosic biomass, but its high cost presents the biggest challenge. In this article, low-cost mixture was produced from glucose through the transglycosylation reaction catalyzed by β-glucosidase for cellulase overproduction by Trichodema reesei RUT C30. As a result, cellulase titer of 90.3FPU/mL, which was more than 10 folds of that achieved with lactose as inducer, was achieved at 144h. Meanwhile, cellulase productivity was drastically increased to 627.1FPU/L/h, at least 3-5 folds higher than previously reported by the fungal species. The crude enzyme was further tested by hydrolyzing NaOH-pretreated corn stover with 15% solid loading, and 96.6g/L glucose was released with 92.6% sugar yield at 96h and 44.8g/L ethanol was obtained.

Authors+Show Affiliations

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China. Electronic address: yh_li@mail.dlut.edu.cn.State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: cg.liu@sjtu.edu.cn.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China; State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: fwbai@sjtu.edu.cn.State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xqzhao@sjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27268435

Citation

Li, Yonghao, et al. "Overproduction of Cellulase By Trichoderma Reesei RUT C30 Through Batch-feeding of Synthesized Low-cost Sugar Mixture." Bioresource Technology, vol. 216, 2016, pp. 503-10.
Li Y, Liu C, Bai F, et al. Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding of synthesized low-cost sugar mixture. Bioresour Technol. 2016;216:503-10.
Li, Y., Liu, C., Bai, F., & Zhao, X. (2016). Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding of synthesized low-cost sugar mixture. Bioresource Technology, 216, 503-10. https://doi.org/10.1016/j.biortech.2016.05.108
Li Y, et al. Overproduction of Cellulase By Trichoderma Reesei RUT C30 Through Batch-feeding of Synthesized Low-cost Sugar Mixture. Bioresour Technol. 2016;216:503-10. PubMed PMID: 27268435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding of synthesized low-cost sugar mixture. AU - Li,Yonghao, AU - Liu,Chenguang, AU - Bai,Fengwu, AU - Zhao,Xinqing, Y1 - 2016/05/28/ PY - 2016/04/17/received PY - 2016/05/25/revised PY - 2016/05/26/accepted PY - 2016/6/9/entrez PY - 2016/6/9/pubmed PY - 2017/1/20/medline KW - Cellulase production KW - Enzymatic hydrolysis of pretreated corn stover KW - Fed-batch fermentation KW - Trichodema reesei SP - 503 EP - 10 JF - Bioresource technology JO - Bioresour. Technol. VL - 216 N2 - Cellulase is a prerequisite for the bioconversion of lignocellulosic biomass, but its high cost presents the biggest challenge. In this article, low-cost mixture was produced from glucose through the transglycosylation reaction catalyzed by β-glucosidase for cellulase overproduction by Trichodema reesei RUT C30. As a result, cellulase titer of 90.3FPU/mL, which was more than 10 folds of that achieved with lactose as inducer, was achieved at 144h. Meanwhile, cellulase productivity was drastically increased to 627.1FPU/L/h, at least 3-5 folds higher than previously reported by the fungal species. The crude enzyme was further tested by hydrolyzing NaOH-pretreated corn stover with 15% solid loading, and 96.6g/L glucose was released with 92.6% sugar yield at 96h and 44.8g/L ethanol was obtained. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/27268435/Overproduction_of_cellulase_by_Trichoderma_reesei_RUT_C30_through_batch_feeding_of_synthesized_low_cost_sugar_mixture_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(16)30767-2 DB - PRIME DP - Unbound Medicine ER -