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The use of Ocimum americanum essential oil against the pathogens Aeromonas hydrophila and Gyrodactylus sp. in silver catfish (Rhamdia quelen).
Lett Appl Microbiol 2016; 63(2):82-8LA

Abstract

The bactericidal activity (minimum inhibitory concentration (MIC)-test) of Ocimum americanum (inflorescences) essential oil (OAEO) against Aeromonas hydrophila was determined in this study. Also investigated was the potential of OAEO and the main compound found in the oil (linalool) at subinhibitory concentrations to be inhibitors of haemolysis caused by Aer. hydrophila in fish erythrocytes. An in vivo experiment was conducted to evaluate the survival of fish (Rhamdia quelen) experimentally infected with Aer. hydrophila and exposed to OAEO. A second experiment was conducted to evaluate the in vitro and in vivo activity of OAEO (mix from inflorescences and leaves) against the parasite Gyrodactylus sp. The OAEO showed weak in vitro activity against Aer. hydrophila (6400 μg ml(-1)). Subinhibitory concentrations of OAEO (100 μg ml(-1)) inhibited haemolysis (90%) caused by Aer. hydrophila in fish erythrocytes, however, linalool did not inhibit haemolysis activity. At the low concentrations (10 and 20 mg l(-1)) added to the water, OAEO promoted the survival of fish experimentally infected with Aer. hydrophila. Lastly, the OAEO mix (50 mg l(-1)) was effective against Gyrodactylus sp., significantly reducing (60%) the number of parasites in the fish.

SIGNIFICANCE AND IMPACT OF THE STUDY

Phytochemicals, such as essential oils (EOs) are a great source of new molecules and have shown potential to be used in aquaculture systems. However, additional studies focused on the in vivo efficacy, mode of action and identification of the active compounds are needed. This study determined the potential of Ocimum americanum EO for use against two important fish pathogens, Aeromonas hydrophila and Gyrodactylus sp., as well as providing preliminary information about the role of the main EO compound (linalool) against Aer. hydrophila virulence.

Authors+Show Affiliations

Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Industrial Pharmacy, UFSM, Santa Maria, RS, Brazil.Regional Integrated University of High Uruguay and Missions (URI), Santiago, RS, Brazil.Department of Preventive Veterinary Medicine, UFSM, Santa Maria, RS, Brazil.Department of Industrial Pharmacy, UFSM, Santa Maria, RS, Brazil.Department of Preventive Veterinary Medicine, UFSM, Santa Maria, RS, Brazil.Department of Agronomy, UFSM/CESNORS, Frederico Westphalen, RS, Brazil.Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27270753

Citation

Sutili, F J., et al. "The Use of Ocimum Americanum Essential Oil Against the Pathogens Aeromonas Hydrophila and Gyrodactylus Sp. in Silver Catfish (Rhamdia Quelen)." Letters in Applied Microbiology, vol. 63, no. 2, 2016, pp. 82-8.
Sutili FJ, Murari AL, Silva LL, et al. The use of Ocimum americanum essential oil against the pathogens Aeromonas hydrophila and Gyrodactylus sp. in silver catfish (Rhamdia quelen). Lett Appl Microbiol. 2016;63(2):82-8.
Sutili, F. J., Murari, A. L., Silva, L. L., Gressler, L. T., Heinzmann, B. M., de Vargas, A. C., ... Baldisserotto, B. (2016). The use of Ocimum americanum essential oil against the pathogens Aeromonas hydrophila and Gyrodactylus sp. in silver catfish (Rhamdia quelen). Letters in Applied Microbiology, 63(2), pp. 82-8. doi:10.1111/lam.12602.
Sutili FJ, et al. The Use of Ocimum Americanum Essential Oil Against the Pathogens Aeromonas Hydrophila and Gyrodactylus Sp. in Silver Catfish (Rhamdia Quelen). Lett Appl Microbiol. 2016;63(2):82-8. PubMed PMID: 27270753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of Ocimum americanum essential oil against the pathogens Aeromonas hydrophila and Gyrodactylus sp. in silver catfish (Rhamdia quelen). AU - Sutili,F J, AU - Murari,A L, AU - Silva,L L, AU - Gressler,L T, AU - Heinzmann,B M, AU - de Vargas,A C, AU - Schmidt,D, AU - Baldisserotto,B, PY - 2016/04/19/received PY - 2016/05/24/revised PY - 2016/06/02/accepted PY - 2016/6/9/entrez PY - 2016/6/9/pubmed PY - 2017/5/13/medline KW - haemolysis KW - linalool KW - monogenea KW - plant KW - virulence SP - 82 EP - 8 JF - Letters in applied microbiology JO - Lett. Appl. Microbiol. VL - 63 IS - 2 N2 - UNLABELLED: The bactericidal activity (minimum inhibitory concentration (MIC)-test) of Ocimum americanum (inflorescences) essential oil (OAEO) against Aeromonas hydrophila was determined in this study. Also investigated was the potential of OAEO and the main compound found in the oil (linalool) at subinhibitory concentrations to be inhibitors of haemolysis caused by Aer. hydrophila in fish erythrocytes. An in vivo experiment was conducted to evaluate the survival of fish (Rhamdia quelen) experimentally infected with Aer. hydrophila and exposed to OAEO. A second experiment was conducted to evaluate the in vitro and in vivo activity of OAEO (mix from inflorescences and leaves) against the parasite Gyrodactylus sp. The OAEO showed weak in vitro activity against Aer. hydrophila (6400 μg ml(-1)). Subinhibitory concentrations of OAEO (100 μg ml(-1)) inhibited haemolysis (90%) caused by Aer. hydrophila in fish erythrocytes, however, linalool did not inhibit haemolysis activity. At the low concentrations (10 and 20 mg l(-1)) added to the water, OAEO promoted the survival of fish experimentally infected with Aer. hydrophila. Lastly, the OAEO mix (50 mg l(-1)) was effective against Gyrodactylus sp., significantly reducing (60%) the number of parasites in the fish. SIGNIFICANCE AND IMPACT OF THE STUDY: Phytochemicals, such as essential oils (EOs) are a great source of new molecules and have shown potential to be used in aquaculture systems. However, additional studies focused on the in vivo efficacy, mode of action and identification of the active compounds are needed. This study determined the potential of Ocimum americanum EO for use against two important fish pathogens, Aeromonas hydrophila and Gyrodactylus sp., as well as providing preliminary information about the role of the main EO compound (linalool) against Aer. hydrophila virulence. SN - 1472-765X UR - https://www.unboundmedicine.com/medline/citation/27270753/The_use_of_Ocimum_americanum_essential_oil_against_the_pathogens_Aeromonas_hydrophila_and_Gyrodactylus_sp__in_silver_catfish__Rhamdia_quelen__ L2 - https://doi.org/10.1111/lam.12602 DB - PRIME DP - Unbound Medicine ER -