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The combination of blueberry juice and probiotics reduces apoptosis of alcoholic fatty liver of mice by affecting SIRT1 pathway.
Drug Des Devel Ther 2016; 10:1649-61DD

Abstract

PURPOSE

To explore the effects of the combination of blueberry juice and probiotics on the apoptosis of alcoholic fatty liver disease (AFLD).

METHODS

Healthy C57BL/6J mice were used in the control group (CG). AFLD mice models were established with Lieber-DeCarli ethanol diet and evenly assigned to six groups with different treatments: MG (model), SI (SIRT1 [sirtuin type 1] small interfering RNA [siRNA]), BJ (blueberry juice), BJSI (blueberry juice and SIRT1 siRNA), BJP (blueberry juice and probiotics), and BJPSI (blueberry juice, probiotics, and SIRT1 siRNA). Hepatic tissue was observed using hematoxylin and eosin (HE) and Oil Red O (ORO) staining. Biochemical indexes of the blood serum were analyzed. The levels of SIRT1, caspase-3, forkhead box protein O1 (FOXO1), FasL (tumor necrosis factor ligand superfamily member 6), BAX, and Bcl-2 were measured by reverse transcription-polymerase chain reaction and Western blotting.

RESULTS

HE and ORO staining showed that the hepatocytes were heavily destroyed with large lipid droplets in MG and SI groups, while the severity was reduced in the CG, BJ, and BJP groups (P<0.05). The levels of superoxide dismutase (SOD), reduced glutathione (GSH), and high-density lipoprotein-cholesterol (HDL-C) were increased in BJ and BJP groups when compared with the model group (P<0.05). In contrast, the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), total triglycerides (TGs), total cholesterol, low-density lipoprotein-cholesterol (LDL-C), and malondialdehyde (MDA) were lower in BJ and BJP groups than in the model group (P<0.05). The level of SIRT1 was increased, while the levels of FOXO1, phosphorylated FOXO1, acetylated FOXO1, FasL, caspase-3, BAX, and Bcl-2 were decreased in CG, BJ, and BJP groups (P<0.05). Meanwhile, SIRT1 silence resulted in increase of the levels of FOXO1, phosphorylated FOXO1, acetylated FOXO1, FasL, caspase-3, BAX, and Bcl-2.

CONCLUSION

The combination of blueberry juice and probiotics reduces apoptosis in AFLD by suppressing FOXO1, phosphorylated FOXO1, acetylated FOXO1, FasL, caspase-3, BAX, and Bcl-2 via the upregulation of SIRT1.

Authors+Show Affiliations

First Hospital Affiliated to Suzhou University, Suzhou, People's Republic of China; Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College, Guiyang, People's Republic of China.Biochemistry Department, Affiliated Hospital of Guiyang Medical College, Guiyang, People's Republic of China.Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College, Guiyang, People's Republic of China.Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College, Guiyang, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27274198

Citation

Zhu, Juanjuan, et al. "The Combination of Blueberry Juice and Probiotics Reduces Apoptosis of Alcoholic Fatty Liver of Mice By Affecting SIRT1 Pathway." Drug Design, Development and Therapy, vol. 10, 2016, pp. 1649-61.
Zhu J, Ren T, Zhou M, et al. The combination of blueberry juice and probiotics reduces apoptosis of alcoholic fatty liver of mice by affecting SIRT1 pathway. Drug Des Devel Ther. 2016;10:1649-61.
Zhu, J., Ren, T., Zhou, M., & Cheng, M. (2016). The combination of blueberry juice and probiotics reduces apoptosis of alcoholic fatty liver of mice by affecting SIRT1 pathway. Drug Design, Development and Therapy, 10, pp. 1649-61. doi:10.2147/DDDT.S102883.
Zhu J, et al. The Combination of Blueberry Juice and Probiotics Reduces Apoptosis of Alcoholic Fatty Liver of Mice By Affecting SIRT1 Pathway. Drug Des Devel Ther. 2016;10:1649-61. PubMed PMID: 27274198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The combination of blueberry juice and probiotics reduces apoptosis of alcoholic fatty liver of mice by affecting SIRT1 pathway. AU - Zhu,Juanjuan, AU - Ren,Tingting, AU - Zhou,Mingyu, AU - Cheng,Mingliang, Y1 - 2016/05/12/ PY - 2016/6/9/entrez PY - 2016/6/9/pubmed PY - 2017/5/10/medline KW - alcoholic fatty liver KW - blueberry KW - caspase-3 KW - forkhead box protein O1 KW - probiotics KW - sirtuin type 1 KW - tumor necrosis factor ligand superfamily member 6 SP - 1649 EP - 61 JF - Drug design, development and therapy JO - Drug Des Devel Ther VL - 10 N2 - PURPOSE: To explore the effects of the combination of blueberry juice and probiotics on the apoptosis of alcoholic fatty liver disease (AFLD). METHODS: Healthy C57BL/6J mice were used in the control group (CG). AFLD mice models were established with Lieber-DeCarli ethanol diet and evenly assigned to six groups with different treatments: MG (model), SI (SIRT1 [sirtuin type 1] small interfering RNA [siRNA]), BJ (blueberry juice), BJSI (blueberry juice and SIRT1 siRNA), BJP (blueberry juice and probiotics), and BJPSI (blueberry juice, probiotics, and SIRT1 siRNA). Hepatic tissue was observed using hematoxylin and eosin (HE) and Oil Red O (ORO) staining. Biochemical indexes of the blood serum were analyzed. The levels of SIRT1, caspase-3, forkhead box protein O1 (FOXO1), FasL (tumor necrosis factor ligand superfamily member 6), BAX, and Bcl-2 were measured by reverse transcription-polymerase chain reaction and Western blotting. RESULTS: HE and ORO staining showed that the hepatocytes were heavily destroyed with large lipid droplets in MG and SI groups, while the severity was reduced in the CG, BJ, and BJP groups (P<0.05). The levels of superoxide dismutase (SOD), reduced glutathione (GSH), and high-density lipoprotein-cholesterol (HDL-C) were increased in BJ and BJP groups when compared with the model group (P<0.05). In contrast, the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), total triglycerides (TGs), total cholesterol, low-density lipoprotein-cholesterol (LDL-C), and malondialdehyde (MDA) were lower in BJ and BJP groups than in the model group (P<0.05). The level of SIRT1 was increased, while the levels of FOXO1, phosphorylated FOXO1, acetylated FOXO1, FasL, caspase-3, BAX, and Bcl-2 were decreased in CG, BJ, and BJP groups (P<0.05). Meanwhile, SIRT1 silence resulted in increase of the levels of FOXO1, phosphorylated FOXO1, acetylated FOXO1, FasL, caspase-3, BAX, and Bcl-2. CONCLUSION: The combination of blueberry juice and probiotics reduces apoptosis in AFLD by suppressing FOXO1, phosphorylated FOXO1, acetylated FOXO1, FasL, caspase-3, BAX, and Bcl-2 via the upregulation of SIRT1. SN - 1177-8881 UR - https://www.unboundmedicine.com/medline/citation/27274198/The_combination_of_blueberry_juice_and_probiotics_reduces_apoptosis_of_alcoholic_fatty_liver_of_mice_by_affecting_SIRT1_pathway_ L2 - https://dx.doi.org/10.2147/DDDT.S102883 DB - PRIME DP - Unbound Medicine ER -