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A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity.
Med Microbiol Immunol. 2016 Oct; 205(5):435-47.MM

Abstract

The authors report the biological synthesis of silver nanoparticles (AgNPs) by alkaliphilic actinobacterium Nocardiopsis valliformis OT1 strain isolated for the first time from Lonar crater, India. The primary detection of silver NPs formation was made by colour change from colourless to dark brown and confirmed by UV-Vis spectrum of AgNPs at 423 nm, specific for AgNPs. Further, AgNPs were characterized by nanoparticle tracking analysis, Zeta sizer, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analyses. FTIR analysis showed the presence of proteins as capping agent. TEM analysis revealed the formation of spherical and polydispersed AgNPs within the size range of 5-50 nm. The antimicrobial activity of silver nanoparticles against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was evaluated. The AgNPs showed the maximum antibacterial activity against B. subtilis (Gram positive) and the minimum against E. coli (Gram negative). The minimal inhibitory concentration values of AgNPs for the tested bacteria were found to be in the range of 30-80 µg/mL. The AgNPs demonstrated higher antibacterial activity against all the bacteria tested as compared with the commercially available antibiotics. The cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) demonstrated a dose-response activity. The IC50 value was found to be 100 µg/mL of AgNPs against cancer HeLa cell line.

Authors+Show Affiliations

Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland. Nanobiotechnology Lab, Department of Biotechnology, SGB Amravati University, Amravati, Maharashtra, 444602, India.Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland. golinska@umk.pl.Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland.Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland.Nanobiotechnology Lab, Department of Biotechnology, SGB Amravati University, Amravati, Maharashtra, 444602, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27278909

Citation

Rathod, Dnyaneshwar, et al. "A New Report of Nocardiopsis Valliformis Strain OT1 From Alkaline Lonar Crater of India and Its Use in Synthesis of Silver Nanoparticles With Special Reference to Evaluation of Antibacterial Activity and Cytotoxicity." Medical Microbiology and Immunology, vol. 205, no. 5, 2016, pp. 435-47.
Rathod D, Golinska P, Wypij M, et al. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity. Med Microbiol Immunol. 2016;205(5):435-47.
Rathod, D., Golinska, P., Wypij, M., Dahm, H., & Rai, M. (2016). A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity. Medical Microbiology and Immunology, 205(5), 435-47. https://doi.org/10.1007/s00430-016-0462-1
Rathod D, et al. A New Report of Nocardiopsis Valliformis Strain OT1 From Alkaline Lonar Crater of India and Its Use in Synthesis of Silver Nanoparticles With Special Reference to Evaluation of Antibacterial Activity and Cytotoxicity. Med Microbiol Immunol. 2016;205(5):435-47. PubMed PMID: 27278909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity. AU - Rathod,Dnyaneshwar, AU - Golinska,Patrycja, AU - Wypij,Magdalena, AU - Dahm,Hanna, AU - Rai,Mahendra, Y1 - 2016/06/09/ PY - 2016/01/15/received PY - 2016/06/03/accepted PY - 2016/6/10/entrez PY - 2016/6/10/pubmed PY - 2017/3/23/medline KW - Alkaliphilic actinobacteria KW - Antibacterial activity KW - Biosynthesis KW - Cytotoxicity KW - Silver nanoparticles SP - 435 EP - 47 JF - Medical microbiology and immunology JO - Med Microbiol Immunol VL - 205 IS - 5 N2 - The authors report the biological synthesis of silver nanoparticles (AgNPs) by alkaliphilic actinobacterium Nocardiopsis valliformis OT1 strain isolated for the first time from Lonar crater, India. The primary detection of silver NPs formation was made by colour change from colourless to dark brown and confirmed by UV-Vis spectrum of AgNPs at 423 nm, specific for AgNPs. Further, AgNPs were characterized by nanoparticle tracking analysis, Zeta sizer, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analyses. FTIR analysis showed the presence of proteins as capping agent. TEM analysis revealed the formation of spherical and polydispersed AgNPs within the size range of 5-50 nm. The antimicrobial activity of silver nanoparticles against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was evaluated. The AgNPs showed the maximum antibacterial activity against B. subtilis (Gram positive) and the minimum against E. coli (Gram negative). The minimal inhibitory concentration values of AgNPs for the tested bacteria were found to be in the range of 30-80 µg/mL. The AgNPs demonstrated higher antibacterial activity against all the bacteria tested as compared with the commercially available antibiotics. The cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) demonstrated a dose-response activity. The IC50 value was found to be 100 µg/mL of AgNPs against cancer HeLa cell line. SN - 1432-1831 UR - https://www.unboundmedicine.com/medline/citation/27278909/A_new_report_of_Nocardiopsis_valliformis_strain_OT1_from_alkaline_Lonar_crater_of_India_and_its_use_in_synthesis_of_silver_nanoparticles_with_special_reference_to_evaluation_of_antibacterial_activity_and_cytotoxicity_ DB - PRIME DP - Unbound Medicine ER -