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Activation of myeloid dendritic cells, effector cells and regulatory T cells in lichen planus.
J Transl Med 2016; 14(1):171JT

Abstract

BACKGROUND

Lichen planus (LP) is a chronic mucocutaneous inflammatory disease. Evaluating the balance between regulatory T cells and effector T cells could be useful for monitoring the proinflammatory profile of LP. Therefore, this study aimed to assess populations of dendritic cells (DCs) and regulatory and effector T cells in peripheral blood samples collected from patients with LP to evaluate the polyfunctionality of T cells upon toll-like receptor (TLR) activation.

METHODS

Peripheral blood mononuclear cells collected from 18 patients with LP and 22 healthy control subjects were stimulated with agonists of TLR4, TLR7, TLR7/TLR8 or TLR9. Frequencies of circulating IFN-α(+) plasmacytoid DCs (pDCs); TNF-α(+) myeloid DCs (mDCs); regulatory T cells (Tregs); and IL-17-, IL-10-, IL-22-, TNF-, and IFN-γ-secreting T cells were assessed via flow cytometry.

RESULTS

The frequencies of regulatory CD4(+) and CD8(+)CD25(+)Foxp3(+)CD127(low/-) T cells and TNF-α(+) mDCs were induced following activation with TLR4, TLR7 and TLR8 agonists in the LP group. Moreover, increased baseline frequencies of CD4(+)IL-10(+) T cells and CD8(+)IL-22(+) or IFN-γ(+)T cells were found. In the LP group, TLR4 activation induced an increased frequency of CD4(+)IFN-γ(+) T cells, while TLR7/8 and staphylococcal enterotoxin B (SEB) activation induced an increased frequency of CD8(+) IL-22(+) T cells. An increased frequency of polyfunctional CD4(+) T cells that simultaneously secreted 3 of the evaluated cytokines (not including IL-10) was verified upon TLR7/8/9 activation, while polyfunctional CD8(+) T cells were already detectable at baseline.

CONCLUSIONS

TLR-mediated activation of the innate immune response induced the production of proinflammatory mDCs, Tregs and polyfunctional T cells in patients with LP. Therefore, TLR activation has an adjuvant role in inducing both innate and adaptive immune responses.

Authors+Show Affiliations

Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, Institut of Tropical Medicine of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 500, 3rd floor 24, São Paulo, 05403-000, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, Institut of Tropical Medicine of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 500, 3rd floor 24, São Paulo, 05403-000, Brazil.Dermatological Outpatient Clinic, Hospital das Clínicas, Medical School of the University of São Paulo, São Paulo, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, Institut of Tropical Medicine of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 500, 3rd floor 24, São Paulo, 05403-000, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, Institut of Tropical Medicine of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 500, 3rd floor 24, São Paulo, 05403-000, Brazil. marisato@usp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27286889

Citation

Domingues, Rosana, et al. "Activation of Myeloid Dendritic Cells, Effector Cells and Regulatory T Cells in Lichen Planus." Journal of Translational Medicine, vol. 14, no. 1, 2016, p. 171.
Domingues R, de Carvalho GC, Aoki V, et al. Activation of myeloid dendritic cells, effector cells and regulatory T cells in lichen planus. J Transl Med. 2016;14(1):171.
Domingues, R., de Carvalho, G. C., Aoki, V., da Silva Duarte, A. J., & Sato, M. N. (2016). Activation of myeloid dendritic cells, effector cells and regulatory T cells in lichen planus. Journal of Translational Medicine, 14(1), p. 171. doi:10.1186/s12967-016-0938-1.
Domingues R, et al. Activation of Myeloid Dendritic Cells, Effector Cells and Regulatory T Cells in Lichen Planus. J Transl Med. 2016 06 10;14(1):171. PubMed PMID: 27286889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of myeloid dendritic cells, effector cells and regulatory T cells in lichen planus. AU - Domingues,Rosana, AU - de Carvalho,Gabriel Costa, AU - Aoki,Valéria, AU - da Silva Duarte,Alberto José, AU - Sato,Maria Notomi, Y1 - 2016/06/10/ PY - 2016/02/02/received PY - 2016/06/02/accepted PY - 2016/6/12/entrez PY - 2016/6/12/pubmed PY - 2017/10/24/medline KW - Dendritic cells KW - Lichen planus KW - Polyfunctional T cells KW - Regulatory T cells KW - Toll-like receptor SP - 171 EP - 171 JF - Journal of translational medicine JO - J Transl Med VL - 14 IS - 1 N2 - BACKGROUND: Lichen planus (LP) is a chronic mucocutaneous inflammatory disease. Evaluating the balance between regulatory T cells and effector T cells could be useful for monitoring the proinflammatory profile of LP. Therefore, this study aimed to assess populations of dendritic cells (DCs) and regulatory and effector T cells in peripheral blood samples collected from patients with LP to evaluate the polyfunctionality of T cells upon toll-like receptor (TLR) activation. METHODS: Peripheral blood mononuclear cells collected from 18 patients with LP and 22 healthy control subjects were stimulated with agonists of TLR4, TLR7, TLR7/TLR8 or TLR9. Frequencies of circulating IFN-α(+) plasmacytoid DCs (pDCs); TNF-α(+) myeloid DCs (mDCs); regulatory T cells (Tregs); and IL-17-, IL-10-, IL-22-, TNF-, and IFN-γ-secreting T cells were assessed via flow cytometry. RESULTS: The frequencies of regulatory CD4(+) and CD8(+)CD25(+)Foxp3(+)CD127(low/-) T cells and TNF-α(+) mDCs were induced following activation with TLR4, TLR7 and TLR8 agonists in the LP group. Moreover, increased baseline frequencies of CD4(+)IL-10(+) T cells and CD8(+)IL-22(+) or IFN-γ(+)T cells were found. In the LP group, TLR4 activation induced an increased frequency of CD4(+)IFN-γ(+) T cells, while TLR7/8 and staphylococcal enterotoxin B (SEB) activation induced an increased frequency of CD8(+) IL-22(+) T cells. An increased frequency of polyfunctional CD4(+) T cells that simultaneously secreted 3 of the evaluated cytokines (not including IL-10) was verified upon TLR7/8/9 activation, while polyfunctional CD8(+) T cells were already detectable at baseline. CONCLUSIONS: TLR-mediated activation of the innate immune response induced the production of proinflammatory mDCs, Tregs and polyfunctional T cells in patients with LP. Therefore, TLR activation has an adjuvant role in inducing both innate and adaptive immune responses. SN - 1479-5876 UR - https://www.unboundmedicine.com/medline/citation/27286889/Activation_of_myeloid_dendritic_cells_effector_cells_and_regulatory_T_cells_in_lichen_planus_ L2 - https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-016-0938-1 DB - PRIME DP - Unbound Medicine ER -