Tags

Type your tag names separated by a space and hit enter

Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms.
BMC Complement Altern Med. 2016 Jun 11; 16:175.BC

Abstract

BACKGROUND

Methanol extract of Bauhinia purpurea L. (family Fabaceae) (MEBP) possesses high antioxidant and anti-inflammatory activities and recently reported to exert hepatoprotection against paracetamol (PCM)-induced liver injury in rats. In an attempt to identify the hepatoprotective bioactive compounds in MEBP, the extract was prepared in different partitions and subjected to the PCM-induced liver injury model in rats.

METHODS

Dried MEBP was partitioned successively to obtain petroleum ether (PEBP), ethylacetate (EABP) and aqueous (AQBP) partitions, respectively. All partitions were subjected to in vitro antioxidant (i.e. total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH)- and superoxide-radicals scavenging assay, and oxygen radical absorbance capacity (ORAC) assay) and anti-inflammatory (i.e. lipooxygenase (LOX) and xanthine oxidase (XO) assay) analysis. The partitions, prepared in the dose range of 50, 250 and 500 mg/kg, together with a vehicle (10 % DMSO) and standard drug (200 mg/kg silymarin) were administered orally for 7 consecutive days prior to subjection to the 3 mg/kg PCM-induced liver injury model in rats. Following the hepatic injury induction, blood samples and liver were collected for the respective biochemical parameter and histopathological studies. Body weight changes and liver weight were also recorded. The partitions were also subjected to the phytochemical screening and HPLC analysis.

RESULTS

Of all partitions, EABP possessed high TPC value and demonstrated remarkable antioxidant activity when assessed using the DPPH- and superoxide-radical scavenging assay, as well as ORAC assay, which was followed by AQBP and PEBP. All partitions also showed low anti-inflammatory activity via the LOX and XO pathways. In the hepatoprotective study, the effectiveness of the partitions is in the order of EABP>AQBP>PEBP, which is supported by the microscopic analysis and histopathological scoring. In the biochemical analysis, EABP also exerted the most effective effect by reducing the serum level of alanine transaminase (ALT) and aspartate transaminase (AST) at all doses tested in comparison to the other partitions. Phytochemical screening and HPLC analysis suggested the presence of: flavonoids, condensed tannins and triterpenes in EABP; flavonoids, condensed tannins and saponins in PEBP and; only saponins in AQBP.

CONCLUSION

EABP demonstrates the most effective hepatoprotection against PCM-induced liver injury in rats. This observation could be attributed to its remarkable antioxidant activity and the presence of flavonoids that might probably act synergistically with other biocompounds to cause the hepatoprotection.

Authors+Show Affiliations

Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. dr_zaz@yahoo.com. Pharmacogenomics Centre (PROMISE), Faculty of Pharmacy, Universiti TeknologiMARA, 42300, Puncak Alam, Selangor, Malaysia. dr_zaz@yahoo.com.Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.Department of Pathology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia.Medical Technology Division, Malaysian Nuclear Agency, Bangi, 43000, Kajang, Selangor, Malaysia.Pharmacogenomics Centre (PROMISE), Faculty of Pharmacy, Universiti TeknologiMARA, 42300, Puncak Alam, Selangor, Malaysia.Pharmacogenomics Centre (PROMISE), Faculty of Pharmacy, Universiti TeknologiMARA, 42300, Puncak Alam, Selangor, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27287196

Citation

Zakaria, Zainul Amiruddin, et al. "Hepatoprotective Action of Various Partitions of Methanol Extract of Bauhinia Purpurea Leaves Against Paracetamol-induced Liver Toxicity: Involvement of the Antioxidant Mechanisms." BMC Complementary and Alternative Medicine, vol. 16, 2016, p. 175.
Zakaria ZA, Yahya F, Mamat SS, et al. Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms. BMC Complement Altern Med. 2016;16:175.
Zakaria, Z. A., Yahya, F., Mamat, S. S., Mahmood, N. D., Mohtarrudin, N., Taher, M., Hamid, S. S., Teh, L. K., & Salleh, M. Z. (2016). Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms. BMC Complementary and Alternative Medicine, 16, 175. https://doi.org/10.1186/s12906-016-1110-4
Zakaria ZA, et al. Hepatoprotective Action of Various Partitions of Methanol Extract of Bauhinia Purpurea Leaves Against Paracetamol-induced Liver Toxicity: Involvement of the Antioxidant Mechanisms. BMC Complement Altern Med. 2016 Jun 11;16:175. PubMed PMID: 27287196.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms. AU - Zakaria,Zainul Amiruddin, AU - Yahya,Farhana, AU - Mamat,Siti Syariah, AU - Mahmood,Nur Diyana, AU - Mohtarrudin,Nurhafizah, AU - Taher,Muhammad, AU - Hamid,Siti Selina Abdul, AU - Teh,Lay Kek, AU - Salleh,Mohd Zaki, Y1 - 2016/06/11/ PY - 2015/07/14/received PY - 2016/05/13/accepted PY - 2016/6/12/entrez PY - 2016/6/12/pubmed PY - 2017/1/14/medline KW - Antioxidant KW - Bauhinia purpurea KW - Fabaceae KW - Flavonoids KW - Hepatoprotection KW - Synergistic action SP - 175 EP - 175 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 16 N2 - BACKGROUND: Methanol extract of Bauhinia purpurea L. (family Fabaceae) (MEBP) possesses high antioxidant and anti-inflammatory activities and recently reported to exert hepatoprotection against paracetamol (PCM)-induced liver injury in rats. In an attempt to identify the hepatoprotective bioactive compounds in MEBP, the extract was prepared in different partitions and subjected to the PCM-induced liver injury model in rats. METHODS: Dried MEBP was partitioned successively to obtain petroleum ether (PEBP), ethylacetate (EABP) and aqueous (AQBP) partitions, respectively. All partitions were subjected to in vitro antioxidant (i.e. total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH)- and superoxide-radicals scavenging assay, and oxygen radical absorbance capacity (ORAC) assay) and anti-inflammatory (i.e. lipooxygenase (LOX) and xanthine oxidase (XO) assay) analysis. The partitions, prepared in the dose range of 50, 250 and 500 mg/kg, together with a vehicle (10 % DMSO) and standard drug (200 mg/kg silymarin) were administered orally for 7 consecutive days prior to subjection to the 3 mg/kg PCM-induced liver injury model in rats. Following the hepatic injury induction, blood samples and liver were collected for the respective biochemical parameter and histopathological studies. Body weight changes and liver weight were also recorded. The partitions were also subjected to the phytochemical screening and HPLC analysis. RESULTS: Of all partitions, EABP possessed high TPC value and demonstrated remarkable antioxidant activity when assessed using the DPPH- and superoxide-radical scavenging assay, as well as ORAC assay, which was followed by AQBP and PEBP. All partitions also showed low anti-inflammatory activity via the LOX and XO pathways. In the hepatoprotective study, the effectiveness of the partitions is in the order of EABP>AQBP>PEBP, which is supported by the microscopic analysis and histopathological scoring. In the biochemical analysis, EABP also exerted the most effective effect by reducing the serum level of alanine transaminase (ALT) and aspartate transaminase (AST) at all doses tested in comparison to the other partitions. Phytochemical screening and HPLC analysis suggested the presence of: flavonoids, condensed tannins and triterpenes in EABP; flavonoids, condensed tannins and saponins in PEBP and; only saponins in AQBP. CONCLUSION: EABP demonstrates the most effective hepatoprotection against PCM-induced liver injury in rats. This observation could be attributed to its remarkable antioxidant activity and the presence of flavonoids that might probably act synergistically with other biocompounds to cause the hepatoprotection. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/27287196/Hepatoprotective_action_of_various_partitions_of_methanol_extract_of_Bauhinia_purpurea_leaves_against_paracetamol_induced_liver_toxicity:_involvement_of_the_antioxidant_mechanisms_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1110-4 DB - PRIME DP - Unbound Medicine ER -